| Project/Area Number |
22KJ0427
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| Project/Area Number (Other) |
22J20479 (2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2022) |
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| Section | 国内 |
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| Review Section |
Basic Section 64030:Environmental materials and recycle technology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ALAWI ASEEL 筑波大学, 理工情報生命学術院, 特別研究員(DC1)
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| Project Period (FY) |
2023-03-08 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2024: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2023: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2022: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | glycerol / 1,3-propanediol / 3-hydroxypropionic acid / fermentation / metabolic engineering / overexpression / Citrobacter braakii |
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| Outline of Research at the Start |
This research focuses on the metabolic engineering of microorganisms for the production of useful chemical from glycerol.
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| Outline of Annual Research Achievements |
To increase 1,3-propandiol production titer we aimed at enhancing glycerol consumption of TB-96-dLF by overexpression related enzymes. The overexpression of gdh reactivation factor was examined. Fermentation experiment of constructed strain showed higher 1,3-PD productivity but lower bacterial growth and a long lag phase until the bacteria entered exponential phase.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We expect noticeable improvement of bacterial growth as well as 1,3-propanediol production from glycerol when by enhancing glycerol consumption through overexpression of gdh or its reactivation factor. Our obtained results showed a declined bacterial growth and long lag phase until the bacterial entered exponential phase.
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| Strategy for Future Research Activity |
Since overexpression system using pUG19 plasmid was established, we plan to investigate the production of 3-hydroxypropionic acid from glycerol by overexpressing native or heterologous genes related to this pathway.
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