Novel Function Investigation of Coagulation Factors in Defense System Regarding Infection: From Medaka (Oryzias latipes) to Human (Homo sapiens)
| Project/Area Number |
22KJ1527
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| Project/Area Number (Other) |
21J22606 (2021-2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2021-2022) |
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| Section | 国内 |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Nagoya University |
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Principal Investigator |
MENG QI 名古屋大学, 創薬科学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2023: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Fibrinogen / Medaka / Immune response / Infection study / メダカ / 血液凝固 / 免疫 / Extracellular traps / fibrinogen / anemia / infection / immunity |
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| Outline of Research at the Start |
The purpose of my research is to investigate the role of coagulation factors which can help us to stop bleeding when we got injured in the immune system. Medaka is a fish which also has similar blood circulation and immune system as human, so I use them to study the coagulation in immunity.
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| Outline of Annual Research Achievements |
The purpose of my research is the investigation of the novel function of the fibrinogen in the immune system. I established the fibrinogen knockout medaka which shows anemia with smaller number of red blood cells (RBCs) and more immature RBCs. I tried to use two gram-negative bacteria, Flavobacterium columnare and Aeromonas hydrophila, to infect the medaka (Oryzias latipes) through immersing. However, the expression level of several immune-related factors were not increased after infection in RT-qPCR result. Then, I changed the induction methods to the intraperitoneal injection. In this experiment, the lipopolysaccharide (LPS) which is derived from the gram-negative bacteria cell wall, was injected into medaka intraperitoneally. After injection, the immune response was successfully induced with the elevated expression of several pro-inflammatory cytokines, including IFN-gamma, TNF-alpha, hepcidin, etc. There's also significant difference between wildtype (WT) and fibrinogen gamma knockout (KO) medaka in IL-1 beta and complement C1q subunit C expression upon infection.
In order to investigate the role of fibrinogen and its interacting protein in these immune pathway, the immunoprecipitation WB and mass spectrometry was carried out using WT and KO medaka upon infection. Through the immunoprecipitation using anti-medaka fibrinogen gamma antibody, several possible interacting protein was detected including histone. In order to investigate the function of fibrinogen in interaction with the histone in immune system, further investigation is necessary.
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Report
(3 results)
Research Products
(6 results)
