Development of Ligand-tethered Cu-responsive protein labeling chemical tool for visualizing Cu-release at synaptic cleft
| Project/Area Number |
22KJ1889
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| Project/Area Number (Other) |
22J15226 (2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2022) |
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| Section | 国内 |
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| Review Section |
Basic Section 37010:Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
CHENG RONG 京都大学, 工学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2023: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2022: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | cuprous ion / copper ion / biometal / protein labeling / conditional proteomics / chemical proteomics / bioconjugation |
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| Outline of Research at the Start |
In this research, a synaptic cleft-targeted copper ions-responsive labeling reagent would be developed to detect synaptic Cu release in the brain. The reagents would be synthesized first. The reagents would be evaluated in living cells to verify the proof of principle and assay the Cu-responsive properties. The optimal reagent would then be applied in cultured hippocampal neurons to detect synaptic Cu release in hippocampal neurons upon stimulation. Then LT-CuR would be used to detect synaptic Cu release in the more complicated biological environment, such as in acute brain slices and in vivo.
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| Outline of Annual Research Achievements |
This year, I applied optimal CuR to ATP7A knock out cells and identified labeled proteins by mass spectrometry to study the misregulation of copper ions in living systems. I have also collected data to publish the results of this research, which I have submitted to a journal for review. Optimal CuR structure will be used to develop cuprous ion probes with organelle or synapse cleft localization.
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Report
(2 results)
Research Products
(1 results)
