Budget Amount *help |
¥519,480,000 (Direct Cost: ¥399,600,000、Indirect Cost: ¥119,880,000)
Fiscal Year 2015: ¥89,050,000 (Direct Cost: ¥68,500,000、Indirect Cost: ¥20,550,000)
Fiscal Year 2014: ¥89,050,000 (Direct Cost: ¥68,500,000、Indirect Cost: ¥20,550,000)
Fiscal Year 2013: ¥129,480,000 (Direct Cost: ¥99,600,000、Indirect Cost: ¥29,880,000)
Fiscal Year 2012: ¥105,170,000 (Direct Cost: ¥80,900,000、Indirect Cost: ¥24,270,000)
Fiscal Year 2011: ¥106,730,000 (Direct Cost: ¥82,100,000、Indirect Cost: ¥24,630,000)
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Outline of Final Research Achievements |
In this project, we aimed at complete understanding of the mechanism of active transport and pursued “why the structures of ion pumps have to be so” by carrying out crystal structure analyses of Ca^<2+>- and Na^+-pumps. With the Ca^<2+>-pump, we succeeded in elucidating the long-awaited E1 crystal structure and showed the regulatory mechanism of sarcolipin, an associated regulatory protein. With the Na^+ pump, we succeeded in determining the crystal structure of a Na^+-bound form for the first time, resulting in the elucidation of an intricate mechanism for selecting Na^+. We also determined the crystal structures of the Na^+-pump with various cardiotonic steroids and thereby opened a way towards developing a tissue specific compound. Furthermore, we succeeded in visualising lipid bilayers in the crystals of Ca^<2+>-ATPase in four states and described detailed interactions with phospholipids.
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