| Budget Amount *help |
¥190,320,000 (Direct Cost: ¥146,400,000、Indirect Cost: ¥43,920,000)
Fiscal Year 2015: ¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000)
Fiscal Year 2014: ¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000)
Fiscal Year 2013: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
Fiscal Year 2012: ¥38,090,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥8,790,000)
Fiscal Year 2011: ¥39,390,000 (Direct Cost: ¥30,300,000、Indirect Cost: ¥9,090,000)
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| Outline of Final Research Achievements |
Genotoxicity tests are essential for pre-marketing hazard assessment of newly-developed chemicals. Recent development of analytical equipment and cell biology allow us to make innovation in this area. In this study, we tried to develop innovative genotoxicity tests which focused on DNA damage, mutation and mechanism of chromosomal aberration. As for DNA damage, we modified the protocol of DNA adductome method to make it faster and more sensitive. We also developed a new mutation assay using a single molecule real time DNA sequencer, enabling direct monitoring of mutation frequency in DNA of any samples. We also developed methods to evaluate inhibition of DNA damage response which is the important mechanism for DNA damage independent chromosomal aberration.
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