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Phenotypic Plasticity: integrative study of ecology and molecular developmental biology

Research Project

Project/Area Number 23247004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionHokkaido University

Principal Investigator

Nishimura Kenya  北海道大学, 水産科学研究科(研究院), 准教授 (30222186)

Co-Investigator(Kenkyū-buntansha) MIURA Toru  北海道大学, 地球環境科学研究院, 准教授 (00332594)
KISHIDA Osamu  北海道大学, 北方生物圏フィールド科学センター, 准教授 (00545626)
MICHIMAE Hirofumi  北里大学, 薬学部, 助教 (70447069)
KITANO Jun  国立遺伝学研究所, 集団遺伝学研究系, 教授 (80346105)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsエゾサンショウウオ / 表現型可塑性 / トランスクリプトーム解析 / 表現型多形 / ゲノム / トランスくリプトーム解析 / 幾何学的形態解析 / 形態 / 地域集団 / 表現型多型 / 幾何学的形態計測解析 / トランスクリプトーム / 次世代シーケンサー
Outline of Final Research Achievements

The Hokkaido salamander (Hynobius retardatus) is an endemic species inhabiting in Hokkaido Japan. Larvae of the Hokkaido salamander exhibit phenotypic plasticity that associate with defense against predators, offense to prey and conspecific larvae. The larval phenotypic plasticity is an eligible phenomenon for the integrative study of evolutionary ecology and molecular developmental biology.
In this project, I tried to investigate, (1) morphological changes associated with the phenotypic plasticity by geometric morphometric analysis, (2) the genomic information about the morphological changes with the phenotypic plasticity by a RNAseq analysis, and (3) variations of morphology and morphological changes (developmental reaction norm) and microsatellite polymorphism among five local populations in the whole distribution area of the endemic species.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (13 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Development and characterization of 12 micro satellite markers for the Hokkaido salamander (Hynobius retardatus).2015

    • Author(s)
      Matsunami, M., Igawa, T., Nozawa, M., Michimae, H., Miura, T., and Nishimura, K.
    • Journal Title

      Current Herpetology

      Volume: 23 Pages: 177-181

    • NAID

      130005095175

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Transcriptome analysis of predator- and prey-induced phenotypic plasticity in the Hokkaido salamander (Hynobius retardatus).2015

    • Author(s)
      Matsunami, M., Kitano, J., Kishida, O., Michimae, H., Miura, T., and Nishimura, K.
    • Journal Title

      Molecular Ecology

      Volume: 24 Pages: 3064-3076

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat.2015

    • Author(s)
      Mori, T., Yanagisawa, Y, Kitani Y., Sugiyama, M., Kishida, O., and Nishimura, K.
    • Journal Title

      BMC Genomics

      Volume: 16 Pages: 258-274

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histological and MS spectrometric analyses of the modified tissue of bulgy form tadpoles induced by salamander predation2012

    • Author(s)
      Tsukasa Mori
    • Journal Title

      Biology Open

      Volume: 15 Issue: 4 Pages: 308-317

    • DOI

      10.1242/bio2012604

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Integrative Shape Variations in Phenotypic Plasticity2015

    • Author(s)
      Kinya Nishimura
    • Organizer
      ヨーロッパ進化生物学会
    • Place of Presentation
      ローザンヌ (スイス)
    • Year and Date
      2015-08-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Adaptive dynamics in infinite-dimensional trait spaces with constraints2014

    • Author(s)
      Kinya Nishimura
    • Organizer
      JSMB/SMB (日本数理生物学会/アメリカ数理生物学会) 合同大会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2014-07-28
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Modularity of polyphonic developmental reaction norm2013

    • Author(s)
      Kinya Nishimura
    • Organizer
      European Society of Evolutionary Biology
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2013-08-19 – 2013-08-24
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcriptome analysis of predator- and prey-induced phenotypic plasticity in the Hokkaido Salamander (Hynobius retardants)2013

    • Author(s)
      Matsunami, M., Kishida, O., Kitano, J., Michimae, H., Miura, T., and Nishimura, K.
    • Organizer
      European Society of Evolutionary Biology
    • Place of Presentation
      Lisbon, Portogal
    • Year and Date
      2013-08-19
    • Related Report
      2013 Annual Research Report
  • [Presentation] Preconditioned reaction norm of developmental phenotypic plasticity2011

    • Author(s)
      Nishimura, K.
    • Organizer
      ヨーロッパ進化学会
    • Place of Presentation
      チュービンゲン大学 (ドイツ)
    • Related Report
      2011 Annual Research Report
  • [Presentation] エゾサンショウウオ幼生の可塑的形態の形態測定学的分析

    • Author(s)
      西村欣也
    • Organizer
      日本生態学会
    • Place of Presentation
      静岡県国際会議場 (静岡県静岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] トランスクリプトーム解析で明らかになったエゾサンショウウオの表現型可塑性の分子基盤

    • Author(s)
      松波雅俊
    • Organizer
      日本生態学会
    • Place of Presentation
      静岡県国際会議場 (静岡県静岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] トランスクリプトーム解析で明らかになったエゾサンショウウオの表現型可塑性の分子機構

    • Author(s)
      松波雅俊
    • Organizer
      第4回生命情報科学若手の会
    • Place of Presentation
      基礎生物学研究所 (愛知県岡崎市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] トランスクリプトーム解析によるエゾサンショウウオの表現型可塑性の分子機構の解明

    • Author(s)
      松波雅俊
    • Organizer
      ゲノム・遺伝子相関研究会
    • Place of Presentation
      エクシブ琵琶湖 (滋賀県米原市)
    • Related Report
      2012 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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