| Budget Amount *help |
¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
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| Outline of Final Research Achievements |
Thermosensitive TRPM2 channels expressed in mouse mucosal mast cells were found to participate in antigen-induced extracellular Ca2+ influx and subsequent degranulation. In addition, hydrogen peroxide was found to lower the temperature threshold for TRPM2 activation through methionine oxidation, indicating that temperature threshold for TRPM2 activation is regulated by redox signals that enable channel activity at physiological body temperatures. Loss of TRPM2 attenuated zymosan-evoked macrophage functions, including cytokine release and fever-enhanced phagocytic activity. The hydrogen peroxide-induced TRPM2-sensitization mechanism through oxidation was also found to work in mouse pancreatic  cells where glucose-induced oscillation of intracellular Ca2+ concentrations and insulin release was TRPM2- and redox status-dependent.
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