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Development of education movies demonstrating real-time biophysical phenomena by two photon laser microscopy

Research Project

Project/Area Number 23300284
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science education
Research InstitutionMie University

Principal Investigator

MIZOGUCHI Akira  三重大学, 医学(系)研究科(研究院), 教授 (90181916)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords2光子レーザー顕微鏡 / 生体内細胞観察 / GFPマウス / iPS細胞 / 病気の可視化 / 治療効果の可視化 / 生命科学実感教材の開発 / 超早期癌診断・治療 / 二光子レーザー顕微鏡 / 命科学実感教材の開発 / 癌の診断 / 癌の治療 / 神経疾患の診断
Research Abstract

I have engaged in development of education movies demonstrating real-time biophysical phenomena in vivo and in vitro by two photon laser microscopy. Two photon laser microscopy is a new method based on two photon excitation which generates new photons inside the animal body for visualizing real-time images of cells. These real-time movies demonstrating dynamic phenomena occurring inside our body probably enhance the understanding of students studying biological science. In this study I have obtained the real-time images of normal cells in gastrointestinal tracts, liver, pancreas, kidney, brain, and vessels. I have also observed the real-time images of cancer cells in intestines, which gives me an opportunity to develop a novel method to identify very small cancers at the ultra-early stages. The new method has been submitted as an international patent under the financial support from JST. The education movies obtained in this study will be opened in a free internet service.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (21 results)

All 2014 2013 2012 2011

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 1 results) Patent(Industrial Property Rights) (8 results) (of which Overseas: 2 results)

  • [Journal Article] Sevoflurane in combination with propofol, not thiopental, induces a more robust neuroapoptosis than sevoflurane alone in the neonatal mouse brain2014

    • Author(s)
      Tagawa T, Sakuraba S, Kimura K, Mizoguchi A
    • Journal Title

      J Anesth

      Volume: (in press) Issue: 6 Pages: 815-820

    • DOI

      10.1007/s00540-014-1822-x

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy2014

    • Author(s)
      Shimura T, Tanaka K, Toiyama Y, Okigami M, Ide S, Kitajima T, Kondo S, Saigusa S, Ohi M, Araki T, Inoue Y, Uchida K, Mohri Y, Mizoguchi A, Kusunoki M
    • Journal Title

      Gastric Cancer

      Volume: (in press) Issue: 1 Pages: 109-118

    • DOI

      10.1007/s10120-013-0334-y

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant cochlear hair cell attachments caused by Nectin-3 deficiency result in hair bundle abnormalities.2014

    • Author(s)
      Fukuda T, Kominami K, Wang S, Togashi H, Hirata K, Mizoguchi A, Rikitake Y, Takai Y.
    • Journal Title

      Development.

      Volume: 141 Issue: 2 Pages: 399-409

    • DOI

      10.1242/dev.094995

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intravital imaging of gastrointestinal diseases in preclinical models using two-photon laser scanning microscopy.2013

    • Author(s)
      Tanaka K, Toiyama Y, Inoue Y, Uchida K, Araki T, Mohri Y, Mizoguchi A, Kusunoki M.
    • Journal Title

      Surg Today.

      Volume: 43 Issue: 2 Pages: 123-129

    • DOI

      10.1007/s00595-012-0283-9

    • NAID

      10031167976

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration.2012

    • Author(s)
      Wang, Shujie, et al.
    • Journal Title

      Journal of Cell Biology

      Volume: 199 Issue: 2 Pages: 331-345

    • DOI

      10.1083/jcb.201202041

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct Distribution and Localization of Rho-kinase in Mouse Epithelial, Muscle and Neural Tissues2012

    • Author(s)
      Iizuka, M.
    • Journal Title

      Cell Structure and Function

      Volume: 37 Issue: 2 Pages: 155-175

    • DOI

      10.1247/csf.12018

    • NAID

      130004137587

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo real-time imaging of chemotherapy response on the liver metastatic tumor microenvironment using multiphoton microscopy.2012

    • Author(s)
      Tanaka K et al.
    • Journal Title

      Oncol Rep.

      Volume: 28 Issue: 5 Pages: 1822-1830

    • DOI

      10.3892/or.2012.1983

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Periderm cells covering palatal shelves have tight junctions and their desquamation reduces the polarity of palatal shelf epithelial cells in palatogenesis.2012

    • Author(s)
      Yoshida, M.
    • Journal Title

      Genes Cells

      Volume: 17 Issue: 6 Pages: 455-472

    • DOI

      10.1111/j.1365-2443.2012.01601.x

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy2012

    • Author(s)
      Tanaka K, Morimoto Y, Toiyama Y, Matsushita K, Kawamura M, Koike Y, Okugawa Y, Inoue Y, Uchida K, Araki T, Mizoguchi A, Kusunoki M
    • Journal Title

      J Oncol

      Volume: 2012 Pages: 265487-265487

    • DOI

      10.1155/2012/265487

    • NAID

      120003596966

    • Related Report
      2013 Final Research Report 2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intravital dual-colored visualization of colorectal liver metastasis in living mice using two photon laser scanning microscopy2012

    • Author(s)
      Tanaka K, Morimoto Y, Toiyama Y, Okugawa Y, Inoue Y, Uchida K, Kimura K, Mizoguchi A, Kusunoki M
    • Journal Title

      Microsc Res Tech

      Volume: 75(3) Issue: 3 Pages: 307-315

    • DOI

      10.1002/jemt.21059

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse2011

    • Author(s)
      K Kuroda, S Yamada, M Tanaka, M Iizuka, H Yano, D Mori, D Tsuboi, T Nishioka, T Namba, Y Iizuka, S Kubota, T Nagai, D Ibi, R Wang, A Enomoto, M Isotani-Sakakibara, N Asai, K Kimura, H Kiyonari, T Abe, A Mizoguchi,M Sokabe, M Takahashi, K Yamada, K Kaibuchi
    • Journal Title

      Human molecular genetics

      Volume: 20 Issue: 23 Pages: 4666-4683

    • DOI

      10.1093/hmg/ddr400

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intravital Three-Dimensional Dynamic Pathology of Experimental Colitis in Living Mice Using Two-Photon Laser Scanning Microscopy2011

    • Author(s)
      Morimoto Y, Tanaka K, Toiyama Y, Inoue Y, Araki T, Uchida K, Kimura K, Mizoguchi A, Kusunoki M
    • Journal Title

      J Gastrointest Surg

      Volume: 15(10) Issue: 10 Pages: 1842-1850

    • DOI

      10.1007/s11605-011-1632-5

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo real-time two-photon microscopic imaging of platelet aggregation induced by selective laser irradiation to the endothelium created in the beta-actin-green fluorescent protein transgenic mice2011

    • Author(s)
      Koike Y, Tanaka K, Okugawa Y, Morimoto Y, Toiyama Y, Uchida K, Miki C, Mizoguchi A, Kusunoki M
    • Journal Title

      J Thromb Thrombolysis

      Volume: 32(2) Issue: 2 Pages: 138-145

    • DOI

      10.1007/s11239-011-0600-y

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Patent(Industrial Property Rights)] 生体染色法2014

    • Inventor(s)
      溝口 明, 木村一志, 田中光司, 藤原武志, 王 淑杰, 崔 煌植
    • Industrial Property Rights Holder
      三重大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-03-28
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] Aberrant cochlear hair cell attachments due to nectin-3 deficiency cause hair2014

    • Inventor(s)
      溝口 明他5名
    • Industrial Property Rights Holder
      三重大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-03-28
    • Related Report
      2013 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 多光子レーザ診断治療装置2013

    • Inventor(s)
      溝口 明, 木村一志, 田中光司
    • Industrial Property Rights Holder
      三重大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-075256
    • Filing Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 生体染色剤2013

    • Inventor(s)
      溝口 明, 木村一志, 田中光司
    • Industrial Property Rights Holder
      三重大学
    • Industrial Property Number
      2013-075150
    • Filing Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 腫瘍細胞染色剤2013

    • Inventor(s)
      溝口 明, 木村一志, 田中光司
    • Industrial Property Rights Holder
      三重大学
    • Industrial Property Number
      2013-074953
    • Filing Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 多光子レーザ診断治療装置2013

    • Inventor(s)
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Holder
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体染色剤2013

    • Inventor(s)
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Holder
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 腫瘍細胞染色剤2013

    • Inventor(s)
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Holder
      溝口 明、木村 一志、田中 光司
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-29
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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