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Study on new source of volatile organoiodine compounds by isolation and culture of marine microorganisms

Research Project

Project/Area Number 23310010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionNihon University

Principal Investigator

HASHIMOTO Shinya  日本大学, 文理学部, 教授 (10228413)

Co-Investigator(Kenkyū-buntansha) TANI Yukinori  静岡県立大学, 環境科学研究所, 准教授 (10285190)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Keywords植物プランクトン / バクテリア / ハロカーボン / ジヨードメタン / 海洋植物プランクトン / 揮発性有機ヨウ素化合物 / ピコ植物プランクトン
Research Abstract

The isolation and culture method for marine microorganisms was optimized using a flow cytometer. Bacteria were investigated for the production of halocarbon from natural waters. Bacteria were isolated and incubated at 25ºC, and the concentrations of methyl halides (methyl chloride, methyl bromide, methyl iodide) in the gas phase above cultured samples were determined using dynamic headspace gas chromatography/mass spectrometry. Bacteria isolated from brackish water were identified by 16 Svedberg unit ribosomal-ribonucleic acid (16S rRNA) gene sequence analysis. Five genetically different strains of bacteria belonging to Erythrobacter or Pseudomonas produced methyl chloride, methyl bromide, and methyl iodide for several days in the culture. Maximum production rates for methyl halide were observed in the culture of isolated bacteria (HKF-1) belonging to Erythrobacter. Aquatic bacteria could be a new source of methyl halides in marine environments.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (43 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (33 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] Simultaneous determination of 15 halocarbons at pico- to nano-mol per liter levels in water and biological samples using dynamic headspace extraction and gas chromatography- mass-spectrometry2013

    • Author(s)
      Gen Taniai, Hiroshi Oda, Yuki Yoneyama, Minami Abe, Takashi Yamakoshi, Keisuke Ambiru, Michiko Kurihara, Shinya Hashimoto
    • Journal Title

      Proceedings of The Institute of Natural Sciences Nihon University

      Volume: 48 Pages: 231-242

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of 15 halocarbons at pico- to nano-mol per liter levels in water and biological samples using dynamic headspace extraction and gas chromatography mass-spectrometry2013

    • Author(s)
      Gen Taniai, Hiroshi Oda, Yuki Yoneyama, Minami Abe, Takashi Yamakoshi, Keisuke Ambiru, Michiko Kurihara, Shinya Hashimoto
    • Journal Title

      Proceedings of The Institute of Natural Sciences Nihon University

      Volume: 48 Pages: 231-242

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brominated methane compounds and isoprene in surface seawater of Sagami Bay : Concentrations, fluxes, and relationships with phytoplankton assemblages2012

    • Author(s)
      Michiko Kurihara, Motoko Iseda, Teru Ioriya, Naho Horimoto, Jota Kanda, Takashi Ishimaru, Yukuya Yamaguchi, Shinya Hashimoto
    • Journal Title

      Marine Chemistry

      Volume: 134- - 135 Pages: 71-79

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methyl halide production by culture of marine proteobacteria Erythrobacter, Pseudomonas and isolated bacteria from brackish water2012

    • Author(s)
      Takuya Fujimori, Yuki Yoneyama, Gen Taniai, Michiko Kurihara, Hideyuki Tamegai, Shinya Hashimoto
    • Journal Title

      Limnology & Oceanography

      Volume: 57 Issue: 1 Pages: 154-162

    • DOI

      10.4319/lo.2012.57.1.0154

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of historical trend of carotenoid concentrations in sediment cores from Lake Shinji2012

    • Author(s)
      K. Kusunoki, M. Sakata, Y. Tani, Y. Seike and K. Ayukawa
    • Journal Title

      Geochemical Journal

      Volume: 46 Pages: 225-233

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Concurrent sorption of As(V) and Mn(II) during biogenic manganese oxide formation2012

    • Author(s)
      J. Watanabe
    • Journal Title

      Chemical Geology

      Volume: 306-307 Pages: 123-128

    • DOI

      10.1016/j.chemgeo.2012.03.004

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brominated methane compounds and isoprene in surface seawater of Sagami Bay:Concentrations, fluxes, and relationships with phytoplankton assemblages2012

    • Author(s)
      Michiko Kurihara, Motoko Iseda, Teru Ioriya, Naho Horimoto, Jota Kanda, Takashi Ishimaru, Masaya Yamaguchi, Shinya Hashimoto
    • Journal Title

      Marine Chemistry

      Volume: 134-135 Pages: 71-79

    • DOI

      10.1016/j.marchem.2012.04.001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brominated methane compounds and isoprene in surface seawater of Sagami Bay : Concentrations, fluxes, and relationships with phytoplankton assemblages

    • Author(s)
      Michiko Kurihara, Motoko Iseda, Teru Ioriya, Naho Horimoto, Jota Kanda, Takashi Ishimaru, Yukuya Yamaguchi, Shinya Hashimoto
    • Journal Title

      Marine Chemistry

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] フローサイトメーターによる汽水湖試料からの植物プランクトンの分取と培養2013

    • Author(s)
      小泉亮,橋本伸哉
    • Organizer
      第14回極限環境生物学会年会
    • Place of Presentation
      明治大学生田校舎, 東京
    • Year and Date
      2013-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 珪藻による揮発性有機化合物の生成2013

    • Author(s)
      柴崎彩人, 安蒜啓佑, 橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学, 札幌
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 緑藻によるテルペン類の生成2013

    • Author(s)
      阿部皆美, 安蒜啓佑, 橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学, 札幌
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 珪藻Chaetoceros sp.によるCH_3ClとCH_3Brの生成2013

    • Author(s)
      西 悠, 安蒜啓祐, 橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学, 札幌
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Volatile Organic Compounds Production from Cultures of T halassiosira pseudonana2013

    • Author(s)
      Ayato Shibazaki, Noboru Enomoto, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Year and Date
      2013-02-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Production of methyl chloride and methyl bromide by Chaetoceros sp.2013

    • Author(s)
      Haruka Nishi, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Year and Date
      2013-02-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Production of volatile organic compounds in the culture of marine bacteria2013

    • Author(s)
      Kenichi Ichikawa, Yuki Yoneyama, Michiko Kurihara, Hideyuki Tamegai, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Year and Date
      2013-02-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Survey of volatile organic compounds in cultures of Chlamydomonas2013

    • Author(s)
      Minami Abe, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Year and Date
      2013-02-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 微生物によるハロカーボンの生成2012

    • Author(s)
      橋本伸哉, 栗原路子
    • Organizer
      第13回極限環境生物学会年会
    • Place of Presentation
      日本大学文理学部・東京
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリアによるモノハロメタンの生成2012

    • Author(s)
      米山祐樹, 藤森拓哉, 谷合元, 栗原路子, 為我井秀行, 橋本伸哉
    • Organizer
      第13回極限環境生物学会年会
    • Place of Presentation
      日本大学文理学部・東京
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] シアノバクテリア Synechococcus によるモノハロメタンの生成2012

    • Author(s)
      安蒜啓佑, 栗原路子, 為我井秀行, 橋本伸哉
    • Organizer
      第13回極限環境生物学会年会
    • Place of Presentation
      日本大学文理学部・東京
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 珪藻 Thalassiosira pseudonana による揮発性有機化合物の生成2012

    • Author(s)
      柴崎彩人, 栗原路子, 為我井秀行, 橋本伸哉
    • Organizer
      第13回極限環境生物学会年会
    • Place of Presentation
      日本大学文理学部・東京
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] シアノバクテリア Synechococcusによるイソプレンの生成2012

    • Author(s)
      土屋海, 栗原路子, 為我井秀行, 橋本伸哉
    • Organizer
      第13回極限環境生物学会年会
    • Place of Presentation
      日本大学文理学部・東京
    • Year and Date
      2012-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Methyl chloride production by two marine cyanobacteria : Synechococcus sp. (CCMP 1334) and Synechococcus sp. (NIES 981)2012

    • Author(s)
      Keisuke Ambiru, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO Summer Meeting 2012
    • Place of Presentation
      滋賀県大津市
    • Year and Date
      2012-07-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Methyl halide production by marine proteobacteria2012

    • Author(s)
      Yuki Yoneyama, Takuya Fujimori, Gen Taniai, Michiko, Kurihara, Hideyuki Tamegai, Shinya Hashimoto
    • Organizer
      ASLO Summer Meeting 2012
    • Place of Presentation
      滋賀県大津市
    • Year and Date
      2012-07-12
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリアによるモノハロメタンの生成2012

    • Author(s)
      米山祐樹, 藤森拓哉, 谷合元, 栗原路子, 為我井秀行, 橋本伸哉
    • Organizer
      日本海洋学会春季大会
    • Place of Presentation
      筑波大学
    • Year and Date
      2012-03-27
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Dynamic head space GC-MS analysis for halomethanes in liquid and viscous samples2011

    • Author(s)
      Shinya Hashimoto, Gen Taniai, Takuya Fujimori, Michiko Kurihara
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2011-05-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dynamic head space GC-MS analysis for halomethanes in liquid and viscous samples2011

    • Author(s)
      Shinya Hashimoto, Gen Taniai, Takuya Fujimori, Michiko Kurihara
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto International Conference Center(招待講演)
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 珪藻による揮発性有機化合物の生成

    • Author(s)
      柴﨑彩人, 安蒜啓佑, 橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学,札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 緑藻によるテルペン類の生成

    • Author(s)
      阿部皆美、安蒜啓佑、橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学,札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 珪藻Chaetoceros sp.によるCH3ClとCH3Brの生成

    • Author(s)
      西 悠、安蒜啓祐、橋本伸哉
    • Organizer
      2013年度日本海洋学会秋季大会
    • Place of Presentation
      北海道大学,札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] フローサイトメーターによる汽水湖試料からの植物プランクトンの分取と培養

    • Author(s)
      小泉亮、橋本伸哉
    • Organizer
      第14 回極限環境生物学会年会
    • Place of Presentation
      明治大学生田校舎、東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Methyl chloride production by two marine cyanobacteria:Synechococcus sp. (CCMP 1334) and Synechococcus sp. (NIES 981)

    • Author(s)
      Keisuke Ambiru, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO Summer Meeting 2012
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] Methyl halide production by marine proteobacteria

    • Author(s)
      Yuki Yoneyama, Takuya Fujimori, Gen Taniai, Michiko, Kurihara, Hideyuki Tamegai, Shinya Hashimoto
    • Organizer
      ASLO Summer Meeting 2012
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物によるハロカーボンの生成

    • Author(s)
      橋本伸哉、栗原路子
    • Organizer
      第13 回極限環境生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] バクテリアによるモノハロメタンの生成

    • Author(s)
      米山祐樹、藤森拓哉、谷合元、栗原路子、為我井秀行、橋本伸哉
    • Organizer
      第13 回極限環境生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] シアノバクテリアSynechococcusによるモノハロメタンの生成

    • Author(s)
      安蒜啓佑、栗原路子、為我井秀行、橋本伸哉
    • Organizer
      第13 回極限環境生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 珪藻Thalassiosira pseudonana による揮発性有機化合物の生成

    • Author(s)
      柴﨑彩人、栗原路子、為我井秀行、橋本伸哉
    • Organizer
      第13 回極限環境生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] シアノバクテリアSynechococcus によるイソプレンの生成

    • Author(s)
      土屋海、栗原路子、為我井秀行、橋本伸哉
    • Organizer
      第13 回極限環境生物学会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Volatile Organic Compounds Production from Cultures of Thalassiosira pseudonana

    • Author(s)
      Ayato Shibazaki, Noboru Enomoto, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2012 Annual Research Report
  • [Presentation] Production of methyl chloride and methyl bromide by Chaetoceros sp.

    • Author(s)
      Haruka Nishi, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2012 Annual Research Report
  • [Presentation] Production of volatile organic compounds in the culture of marine bacteria

    • Author(s)
      Kenichi Ichikawa, Yuki Yoneyama, Michiko Kurihara, Hideyuki Tamegai, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2012 Annual Research Report
  • [Presentation] Survey of volatile organic compounds in cultures of Chlamydomonas. sp

    • Author(s)
      Minami Abe, Michiko Kurihara, Shinya Hashimoto
    • Organizer
      ASLO 2013 Aquatic Science Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2012 Annual Research Report
  • [Book] 藻類ハンドブック「ハロカーボン類を産生する藻類」2012

    • Author(s)
      栗原路子・橋本伸哉
    • Total Pages
      768
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2013 Final Research Report
  • [Book] 藻類ハンドブック, 「ハロカーボン類を産生する藻類」2012

    • Author(s)
      栗原路子, 橋本伸哉
    • Total Pages
      768
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2012 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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