Establishment of the next generation microbial factory for lactate-based polymers by utilizing biomass
| Project/Area Number |
23310059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAGUCHI SEIICHI 北海道大学, 工学(系)研究科(研究院), 教授 (70216828)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Ken'ichiro 北海道大学, 大学院工学研究院, 准教授 (80360642)
佐藤 敏文 北海道大学, 工学(系)研究科(研究院), 教授 (80291235)
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| Co-Investigator(Renkei-kenkyūsha) |
SATOH Toshifumi 北海道大学, 大学院工学研究院, 准教授 (80291235)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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| Keywords | 重合酵素 / 乳酸ポリマー / モノマー組成 / 植物バイオマス / 基質特異性 / 微生物工場 / ポリマー物性 / CoA転移酵素 / 新規モノマー / 酵素進化工学 / コリネ菌 / モノマーフラックス / バイオポリマー / 代謝経路 / 共重合 |
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| Research Abstract |
We have constructed the microbial system for the lactate-based polymers by using our developed lactate-polymerizing enzyme. The copolymers have been synthesized by achieving the range control of lactate fraction, from 0 to 50 mol% in Escherichia coli and 50 to nearly 100 mol% in Corynebacterium glutamicum. Among them, lactate polymer with 30 mol% lactate fraction was produced up to 11 g/L. In terms of polymer properties, copolymers gained flexibility with increasing lactate fraction compared to the rigid hopolymers such as PLA. Also, microbial polymers were demonstrated to be synthesized by using the enzymatically hydrolyzed sugars from the plant biomass.
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Report
(4 results)
Research Products
(129 results)
