| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
Factors involving in translation in wheat were purified one by one, in order to facilitate constructive approaches to the mechanisms in eukaryotic protein synthesis. Samples of acceptable quality were obtained concerning the ribosomes, the 2 eukaryotic elongation factors, 7 of the 14 eukaryotic initiation factors, eukaryotic release factor (eRF) 1, and 4 of the 20 aminoacyl-tRNA synthetases. As no cDNA clone for eRF3 was found in the library of wheat cDNA clones by the National Bio-Resource Project, which had been in progress in parallel with this project, we obtained a clone from wheat germ.
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