| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Research Abstract |
The current study developed a single molecule detection system, which allows the simultaneous observation of the position (x, y, z), orientation (theta, phi), and emission wavelength lambda, as a function of time t. This system enables us to completely record the motion of a single fluorescent molecule.
The dynamics of poly(methyl acrylate) films was examined by the single molecule microscopy system developed here. The rotational motion in an ultra thin film with a thickness of 5 nm was suppressed compared to that in a three-dimensional bulk state. The detailed analysis of the molecular rotation revealed that the rotational relaxation time in the out-of-plane direction was 30-times longer than that in a bulk state and that the in-plane rotation in the ultra-thin film was almost the same as that in a bulk system. This indicates that the molecular rotation in an ultra-thin film is restricted only in the thickness direction.
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