Structural basis and functional evolution of RNA maturation machinary

• Project/Area Number: 23350081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Ehime University
• Principal Investigator: HORI Hiroyuki 愛媛大学, 理工学研究科, 教授 (20256960)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Tsutomu 東京大学, 大学院・工学系研究科, 教授 (20292782) ; HIRATA Akira 愛媛大学, 大学院・理工学研究科, 講師 (60527381)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 核酸 / 酵素 / タンパク質 / ＲＮＡ / RNA / RNA修飾 / RNAメチル化 / tRNA / メチル化酵素 / RNA分解制御 / ゲノム / 構造解析

Research Abstract

The purpose of this study is physical and chemical understanding of RNA maturation system. The study were performed for three years. We mainly researched the relatively simple organism of three domains of life (eukaryotes, archaea and eubacteria). We chracterized several tRNA modification enzymes and tRNA splicing endonulceases by X-ray crystal structural study and biochemical methods. Furthermore, we investigated the network between modified nucleosides in tRNA and tRNA modification enzymes using gene disruptant strains. Moreover, we found distinct tRNA modifications in a thermo-acidophilic archaeon, Thermoplasma acidophilum.

Research Products

• [Journal Article] Methylated nucleosides in tRNA and tRNA methyltransferases (2014)
  • Author(s): H. Hori
  • Journal Title: Frontiers in Genetics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Methylated nucleosides in tRNA and tRNA methyltransferase (2014)
  • Author(s): H. Hori
  • Journal Title: Frontiers in Genetics Volume: in press
  • Peer Reviewed
• [Journal Article] Distinct tRNA modifications in the thermo-acidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): C. Tomikawa, T. Ohira, Y. Inoue, T. Kawamura, A. Yamagishi, T. Suzuki, and H. Hori
  • Journal Title: FEBS Letters 587 Pages: 3575-3580
  • Peer Reviewed
• [Journal Article] The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process (2013)
  • Author(s): A. Ochi, K. Makabe, R. Yamagami, A. Hirata, R. Sakaguchi, Y.M. Hou, K. Watanabe, O. Nureki, K. Kuwajima, and H. Hori
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 25562-25574
  • Peer Reviewed
• [Journal Article] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells (2013)
  • Author(s): K. Watanabe, R. Miyagawa, C. Tomikawa, R. Mizuno, A. Takahashi, H. Hori, and K. Ijiri
  • Journal Title: Nucleic Acids Res Volume: 41 Pages: 4671-4685
  • Peer Reviewed
• [Journal Article] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells (2013)
  • Author(s): Watanabe K, Miyagawa R, Tomikawa C, Mizuno R, Takahashi A, Hori H, Ijiri K.
  • Journal Title: Nucleic Acids Res Volume: 41 Issue: 8 Pages: 4671-4685
  • DOI: 10.1093/nar/gkt153
  • Peer Reviewed
• [Journal Article] The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process. (2013)
  • Author(s): A. Ochi, K. Makabe, R. Yamagami, A. Hirata, R. Sakaguchi, Y.M. Hou, K. Watanabe, O. Nureki, K. Kuwajima, and H. Hori
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 35 Pages: 25562-25574
  • DOI: 10.1074/jbc.m113.485128
  • Peer Reviewed
• [Journal Article] Distinct tRNA modifications in the thermo-acidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): Tomikawa, C., Ohira, T., Inoue, Y., Kawamura, T., Yamagishi, A., Suzuki, T. and Hori, H.
  • Journal Title: FEBS Lett Volume: 587 Issue: 21 Pages: 3575-3580
  • DOI: 10.1016/j.febslet.2013.09.021
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells. (2013)
  • Author(s): K. Watanabe, R. Miyagawa, C. Tomikawa, R. Mizuno, A. Takahashi, H. Hori, and K. Ijiri
  • Journal Title: Nucleic Acids Res. Volume: 41 Pages: 4671-4685
  • Peer Reviewed
• [Journal Article] The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO) (2012)
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 42480-42494
  • Peer Reviewed
• [Journal Article] X-ray structure of the fourth type of archaeal tRNA splicing endonuclease : insight into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity (2012)
  • Author(s): A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfield, A. Kanai, and H. Hori
  • Journal Title: Nucleic Acids Res. Volume: 40 Pages: 10554-10566
  • Peer Reviewed
• [Journal Article] The 2'-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition (2012)
  • Author(s): S. Jöckel, G. Nees, R. Sommer, Y. Zhao, D. Cherkasov, H. Hori, G. Ehm, M. Schnare, M. Nain, A. Kaufmann, and S. Bauer
  • Journal Title: J. Exp. Med Volume: 209 Pages: 235-241
  • Peer Reviewed
• [Journal Article] X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: insight into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity. (2012)
  • Author(s): A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfield, A. Kanai, and H. Hori
  • Journal Title: Nucleic Acids Res. Volume: 40 Issue: 20 Pages: 10554-10566
  • DOI: 10.1093/nar/gks826
  • Peer Reviewed
• [Journal Article] The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO) (2012)
  • Author(s): Yamagami R, Yamashita K, Nishimasu H, Tomikawa C, Ochi A, Iwashita C, Hirata A, Ishitani R, Nureki O, Hori H
  • Journal Title: J Biol Chem Volume: 287 Issue: 51 Pages: 42480-42480
  • DOI: 10.1074/jbc.m112.390112
  • Peer Reviewed
• [Journal Article] The 2'-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition (2012)
  • Author(s): S.Jockel, G.Nees, R.Sommer, Y.Zhao, D.Cherkasov, H.Hon, G.Ehm, M.Schnare, M.Nain, A.Kaufmann, S.Bauer
  • Journal Title: J.Exp.Med. Volume: 209 Issue: 2 Pages: 235-241
  • DOI: 10.1084/jem.20111075
  • Peer Reviewed
• [Journal Article] Substrate tRNA recognition mechanism of a multi-site-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure (2011)
  • Author(s): T. Awai, A. Ochi, Ihsanawati, T. Sengoku, A. Hirata, Y. Bessho, S. Yokoyama, and H. Hori
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 35236-35246
  • Peer Reviewed
• [Journal Article] Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop (2011)
  • Author(s): A. Hirata, T. Kitajima, and H. Hori
  • Journal Title: Nucleic Acids Res. Volume: 39 Pages: 9376-9389
  • Peer Reviewed
• [Journal Article] Pseudouridine at position 55 (Ψ55) in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus (2011)
  • Author(s): K. Ishida, T. Kunibayashi, C. Tomikawa, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Journal Title: Nucleic Acids Res. Volume: 39 Pages: 2304-2318
  • Peer Reviewed
• [Journal Article] Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop (2011)
  • Author(s): A.Hirata, T.Kitajima, H.Hori
  • Journal Title: Nucleic Acids Res Volume: 39 Issue: 21 Pages: 9376-9389
  • DOI: 10.1093/nar/gkr615
  • Peer Reviewed
• [Journal Article] Substrate tRNA recognition mechanism of a multisite-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure (2011)
  • Author(s): Awai T., et al
  • Journal Title: J Biol Chem Volume: 286 Issue: 40 Pages: 35236-35246
  • DOI: 10.1074/jbc.m111.253641
  • Peer Reviewed
• [Presentation] 超好熱性アーキアThermococcus kodakarensis由来tRNATrpの修飾ヌクレオシド分析 (2013)
  • Author(s): 前田雄太, 平田 章, 長野倫子, 土手智裕, 鈴木健夫, 金井保, 鈴木勉, 堀弘幸
  • Organizer: 2013年度極限環境生物学会年会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2013-10-27
• [Presentation] Thermococcus kodakarensis 由来tRNAメチル化酵素(Trm-m22G10)の構造機能解析 (2013)
  • Author(s): 西山聖示, 平田章, 田村俊浩, 堀 弘幸
  • Organizer: 2013年度極限環境生物学会年会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2013-10-27
• [Presentation] Does D20 formation by eubacterial dihydrouridine synthase require the other modified nucleotides for its modification in tRNA? (2013)
  • Author(s): 楠葉浩晃, 吉田剛士, 岩崎絵梨, 粟井貴子, 平田章, 冨川千恵, 山上龍太, 堀弘幸
  • Organizer: 第3回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2013-09-21
• [Presentation] Eukaryotic tRNA (m2G10) methyltransferase (Trm11-Trm112) recognizes the whole structure of tRNA (2013)
  • Author(s): Shiho Ohmori, Yu Nishida, Kazuki Okada, Akira Hirata, and Hiroyuki Hori
  • Organizer: 第3回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2013-09-21
• [Presentation] 固相化DNAプローブ法を用いた好熱菌tRNAの大量精製法の確立 (2013)
  • Author(s): Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: 第3回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2013-09-21
• [Presentation] 葉酸依存性tRNA(m5U54)メチル化酵素TrmFOはどうやってtRNA中のU54を認識しているのか? (2013)
  • Author(s): 山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田章, 石谷隆一郎, 濡木理, 堀 弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] m7G46およびΨ55を中心としたThermus thermophilustRNA修飾ネットワーク (2013)
  • Author(s): 冨川千恵, 石田一雄, 国林貴史, 金井保, 横川隆志, 堀弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] 熱ストレスによる開始tRNAMetの細胞内動態 (2013)
  • Author(s): 渡邉和則, 宮川隆, 冨川千恵, 水野利恵, 高橋昭久, 大槻高史, 堀弘幸, 井尻憲一
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] 真正細菌ジヒドロウリジン合成酵素はD20形成にtRNA上の他の修飾ヌクレオチドを必要とする (2013)
  • Author(s): 楠葉浩晃, 吉田剛士, 岩崎絵梨, 粟井貴子, 平田章, 冨川千恵, 山上龍太, 堀弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] Thermoplasma acidophilum 由来tRNALeuにおいてArchaeosine が2カ所に形成されるメカニズムの解析 (2013)
  • Author(s): 河村卓哉, 冨川千恵, 大平高之 , 井上仁, 行木信一, 岡夏央, 谷口浩輝, 安藤香織, 大野敏, 山岸明彦, 鈴木勉, 横川隆志, 堀弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] 超好熱性アーキアThermococcus kodakarensis 2´-5´RNAリガーゼの機能解析 (2013)
  • Author(s): 平田章, 長野倫子, 石丸雄基, 金井保, 鈴木健夫, 佐藤朝子, 金井昭夫, 鈴木勉, 堀弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] Yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明 (2013)
  • Author(s): 大森志穂, 西田裕, 岡田和樹, 平田章, 堀弘幸
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 松山
  • Year and Date: 2013-07-24
• [Presentation] 極小アーキア(古細菌)における4番目のタイプtRNAスプライシングエンドヌクレアーゼのX線結晶構造 (2013)
  • Author(s): 平田章, 藤島皓介, 山上龍太, 河村卓哉, Jillina F. Banfield, 金井昭夫, 堀弘幸
  • Organizer: 第13回蛋白質科学会
  • Place of Presentation: 鳥取
  • Year and Date: 2013-06-12
• [Presentation] Prediction of genes for tRNA modifications in the thermoacidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): Ryo Anraku, Miyu Higashiguchi, Takuya Kawamura, and Hiroyuki Hori
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Alteration of solid-phase DNA probe method for large-scale tRNA purification (2013)
  • Author(s): Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Novel tRNA modifications in the thermophilic-acidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Takuya Kawamura, Akihiko Yamagishi, Tsutomu Suzuki, and Hiroyuki Hori
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] X-ray crystal structure of the fourth type of tRNA splicing endonuclease from an uncultivated archaeon Candidatus Micrarchaeum acidiphilum (ARMAN-2) (2013)
  • Author(s): Akira Hirata, Kosuke Fujishima, Ryota Yamagami, Takuya Kawamura, Jillian F. Banfiled, Akio Kanai and Hiroyuki Hori
  • Organizer: Thermophiles 2013
  • Place of Presentation: Deutschland
• [Presentation] The synthesis mechanism of a tRNA modification in thermophilic bacteria (2013)
  • Author(s): Takuya Kawamura, Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Nobukazu Nameki, Natsuhisa Oka, Hiroki Taniguchi, Kaori Ando, Satoshi Ohno, Akihiko Yamagishi, Tsutom Suzuki, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: Riboclub 2013 Annual Meeting
  • Place of Presentation: Canada
• [Presentation] 極小アーキア(古細菌）における4番目のタイプtRNAスプライシングエンドヌクレアーゼのX線結晶構造 (2013)
  • Author(s): 平田 章、藤島皓介、山上龍太、河村卓哉、Jillina F. Banfield、金井昭夫、堀 弘幸
  • Organizer: 第１３回蛋白質科学会
  • Place of Presentation: 鳥取
• [Presentation] The synthesis mechanism of a tRNA modification in thermophilic bacteria (2013)
  • Author(s): Takuya Kawamura, Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Nobukazu Nameki, Natsuhisa Oka, Hiroki Taniguchi, Kaori Ando, Satoshi Ohno, Akihiko Yamagishi, Tsutom Suzuki, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: Riboclub 2013 Annual Meeting, Canada
  • Place of Presentation: ケベック、カナダ
• [Presentation] X-ray crystal structure of the fourth type of tRNA splicing endonuclease from an uncultivated archaeon Candidatus Micrarchaeum acidiphilum (ARMAN-2) (2013)
  • Author(s): Akira Hirata, Kosuke Fujishima, Ryota Yamagami, Takuya Kawamura, Jillian F. Banfiled, Akio Kanai and Hiroyuki Hori
  • Organizer: Thermophiles 2013
  • Place of Presentation: レジェンスブルク、ドイツ
• [Presentation] Novel tRNA modifications in the thermophilic-acidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Takuya Kawamura, Akihiko Yamagishi, Tsutomu Suzuki, and Hiroyuki Hori
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Alteration of solid-phase DNA probe method for large-scale tRNA purification (2013)
  • Author(s): Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Prediction of genes for tRNA modifications in the thermoacidophilic archaeon, Thermoplasma acidophilum (2013)
  • Author(s): Ryo Anraku, Miyu Higashiguchi, Takuya Kawamura, and Hiroyuki Hori
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Substrate transfer RNA recognition mechanism of tRNA (m5U54) methyltransferase (TrmFO) based on the biochemical and structural analysis. (2012)
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori
  • Organizer: 第2回モデル生物丸ごと一匹学会
  • Place of Presentation: SPring-8 (Harima)
  • Year and Date: 2012-09-28
• [Presentation] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI) (2012)
  • Author(s): H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori
  • Organizer: 第2回モデル生物丸ごと一匹学会
  • Place of Presentation: SPring-8 (Harima)
  • Year and Date: 2012-09-28
• [Presentation] Functional analysis of yeast tRNA (m2G10) methyltransferase (Trm11-Trm112) (2012)
  • Author(s): Y. Nishida, K. Okada, A. Hirata, and H. Hori
  • Organizer: 第2回モデル生物丸ごと一匹学会
  • Place of Presentation: SPring-8 (Harima)
  • Year and Date: 2012-09-28
• [Presentation] Adaptation of Thermus thermophilus to environmental temperature via tRNA modification enzymes-modified nucleotides network in tRNA (2012)
  • Author(s): C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Organizer: 第2回モデル生物丸ごと一匹学会
  • Place of Presentation: SPring-8 (Harima)
  • Year and Date: 2012-09-28
• [Presentation] Visiting Yellowstone National Park, the place of discovery of thermophiles (2012)
  • Author(s): H. Hori
  • Organizer: 第2回モデル生物丸ごと一匹学会
  • Place of Presentation: SPring-8 (Harima)
  • Year and Date: 2012-09-28
• [Presentation] Transfer RNA modifications in thermophilic bacteria (2012)
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: Spain
  • Year and Date: 2012-09-09
• [Presentation] Transfer RNA recognition mechanism of Thermus thermophilus folate/FAD-dependent tRNA methyltransferase (TrmFO) (2012)
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: Spain
  • Year and Date: 2012-09-09
• [Presentation] Structural insight into the evolution and broad substrate specificity of the fourth type of tRNA splicing endonuclease (2012)
  • Author(s): A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfiled, A. Kanai, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: Spain
  • Year and Date: 2012-09-09
• [Presentation] 極小アーキアにおけるtRNAスプライシングエンドヌクレアーゼの構造機能解析 (2012)
  • Author(s): 平田章, 藤島皓介, 山上龍太, 河村卓哉, J. F. Banfield, 堀弘幸
  • Organizer: 第25回日本アーキア研究会
  • Place of Presentation: 兵庫
  • Year and Date: 2012-07-18
• [Presentation] tRNA modifications in Thermoplasma acidophilum (2012)
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-16
• [Presentation] サーマス・サーモフィラス由来tRNAメチル化酵素TrmFOの基質認識機構 (2012)
  • Author(s): 山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田章, 石谷隆一郎, 濡木理, 堀弘幸
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-16
• [Presentation] 高度好熱菌tRNA中のシュードウリジン55修飾は, 温度変化への適応因子として機能する (2012)
  • Author(s): 冨川千恵, 石田一雄, 国林貴史, 越智杏奈, 金井保, 平田章, 岩下知香子, 堀弘幸
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-16
• [Presentation] RNA修飾成熟マシナリーの構造と機能 (2012)
  • Author(s): 堀弘幸
  • Organizer: 第3回愛媛大学学術フォーラム
  • Place of Presentation: 愛媛県松山市
  • Year and Date: 2012-01-06
• [Presentation] tRNA modifications in thermoacidophilic archaeon (2012)
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明 (2012)
  • Author(s): 西田裕, 岡田和樹, 平田章, 堀弘幸
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Expression and purification of yeast tRNA (Cm32/Gm34) methyltransferase (Trm7) (2012)
  • Author(s): K. Yoshii, A. Hirata, and H. Hori
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Structural and functional analyses of archaeal 2'-5' RNA ligase (2012)
  • Author(s): T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Research for substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI) (2012)
  • Author(s): H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Improvement of a protein purification method for nucleic acid-free in the case of recombinant tRNA (N2, N2-guanine)-dimethyltransferase (Trm1) from Aquifex aeolicus (2012)
  • Author(s): S. Nishiyama, A. Hirata, T. Awai, and H. Hori
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Negative control of Gm18, m5s2U54 and m1A58 modifications by pseudouridine55 modification in Thermus thermophilus tRNAs : the tRNA modification network in extreme thermophile (2012)
  • Author(s): C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Organizer: 24th tRNA workshop
  • Place of Presentation: Chilli
• [Presentation] Thermus thermophilus tRNA (m1A58) methyltransferase [Trm1]の基質認識メカニズムの解明 (2011)
  • Author(s): 詫間浩之, 美濃地真之, 牛尾なつみ, 冨川千恵, 平田章, 堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析 (2011)
  • Author(s): 西田裕, 岡田和樹, 平田章, 堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] 超好熱性古細菌Thermococcus kodakaraensis由来tRNA(m2G10/m22G10) methyltransferase (TK0981)の酵素学的性質の決定 (2011)
  • Author(s): 田村俊浩, 平田章, 堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] サーマス・サーモフィラス由来葉酸依存性tRNA(m5U54)メチル化酵素の基質認識機構 (2011)
  • Author(s): 山上龍太, 山下光輝, 西増弘志, 岩下知香子, 平田章, 濡木理, 堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Thermus thermophilus tRNA (m1A58) methyltransferase[Trm1]の基質認識メカニズムの解明 (2011)
  • Author(s): 詫間浩之、美濃地真之、牛尾なつみ、冨川千恵、平田 章、堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-12-16
• [Presentation] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析 (2011)
  • Author(s): 西田裕、岡田和樹、平田章、堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-12-16
• [Presentation] サーマス・サーモフィラス由来葉酸依存性tRNA (m5U54)メチル化酵素の基質認識機構 (2011)
  • Author(s): 山上龍太、山下光輝、西増弘志、岩下知香子、平田章、濡木理、堀弘幸
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-12-16
• [Presentation] アーキアにおける2'-5' RNA ligaseの構造機能解析 (2011)
  • Author(s): 長野倫子、石丸雄基、金井保、平田章、堀弘幸
  • Organizer: 第12会極限環境生物学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-23
• [Presentation] アーキアRNAポリメラーゼのサブユニットDに見られる特異的ドメイン構造と機能の多様性と進化 (2011)
  • Author(s): 平田章、堀弘幸、村上勝彦、Faith H.Lessner、Matthew Jenning、Eduardus, C.Duin、Daniel J.Lessner
  • Organizer: 第12会極限環境生物学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-23
• [Presentation] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI) (2011)
  • Author(s): Hiroyuki Takuma, Masayuki Minoji, Natsumi Ushio, Chie Tomikawa, Akira Hirata, Anna Ochi, Hiroyuki Hori
  • Organizer: The 38th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 札幌
  • Year and Date: 2011-11-11
• [Presentation] RNA修飾酵素の触媒機構の多様性とそのネットワークの環境応答 (2011)
  • Author(s): 堀弘幸
  • Organizer: 北海道大学グローバルCOE物質科学イノベーション講演会
  • Place of Presentation: 札幌
  • Year and Date: 2011-11-08
• [Presentation] Transfer RNA modifications in Thermoplasma acidophilum (2011)
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] Recognition and cleavage of intron in precursor tRNA by the splicing endonuclease from Aperopyrum pernix, an aerobic hyper-thermophilic Crenarchaeon, thorough a novel RNA recognition site in Crenarchaea specific loop (2011)
  • Author(s): A. Hirata, T. Kitajima, and H. Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] Relationship between 5-methyluridine modification at position 54 in tRNA and tRNA modification network in Thermus thermophiles (2011)
  • Author(s): R. Yamagami, N. Shigi, S.-I. Asai, K. Watanabe, and H. Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] Structural and functional analyses of archaeal 2'-5' RNA ligase (2011)
  • Author(s): T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] Multisite specific tRNA methyltransferase Trm1 from a hyper-thermophilic eubacterium, Aquifex aeolicus (2011)
  • Author(s): T. Awai, A. Ochi, Ihsanawati, T. Sengoku, S. Kimura, C. Tomikawa, T. Yokogawa, A. Hirata, Y. Bessho, T. Suzuki, S. Yokoyama and H. Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] Transfer RNA modification in Thermoplasma acidophilum (2011)
  • Author(s): T.Kawamura, C.Tomikawa, T.Ohira, Y.Inoue, A.Yamagishi, T.Suzuki, H.Hori
  • Organizer: Thermophiles 2011
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2011-09-12
• [Presentation] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析 (2011)
  • Author(s): 西田裕, 岡田和樹, 平田章, 堀弘幸
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: 大府(愛知)
  • Year and Date: 2011-08-30
• [Presentation] 古細菌における2' - 5' RNA ligaseの構造機能解析 (2011)
  • Author(s): 長野倫子, 平田章, 石丸雄基, 金井保, 堀弘幸
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: 大府(愛知)
  • Year and Date: 2011-08-30
• [Presentation] 酵母由来のtRNA (m^2G10) methyltransferase (Trm11-Trm112)の機能解析 (2011)
  • Author(s): 芦田裕、岡田和樹、平田章、堀弘幸
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: 愛知県大府市
  • Year and Date: 2011-08-30
• [Presentation] 古細菌における2'-5' RNA ligaseの構造機能解析 (2011)
  • Author(s): 長野倫子, 平田章, 石丸雄基, 金井保, 堀弘幸
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: 愛知県大府市
  • Year and Date: 2011-08-30
• [Presentation] Elucidation of substrate tRNA recognition mechanism by tRNA (m1A58) methyltransferase[TrmI] from Thermus thermophiles (2011)
  • Author(s): H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, C. Iwashita, A. Ochi, and H. Hori
  • Organizer: 第1回モデル生物丸ごと一匹学会・第10回高度好熱菌連携会議
  • Place of Presentation: 理研・播磨(Spring-8)
  • Year and Date: 2011-08-19
• [Presentation] The crystal structure analysis and establishment of activity mesurement system of Thermus thermophilus tRNA (Um34, Cm34) methyltransferase [YibK] (2011)
  • Author(s): T. Matsugi, A. Hirata, and H. Hori
  • Organizer: 第1回モデル生物丸ごと一匹学会・第10回高度好熱菌連携会議
  • Place of Presentation: 理研・播磨(Spring-8)
  • Year and Date: 2011-08-19
• [Presentation] Elucidation of substrate tRNA recognition mechanism by tRNA (m^1A58)methyltransferase[TrmI]from Thermus thermophiles (2011)
  • Author(s): H.Takuma, M.Minoji, N.Ushio, C.Tomikawa, A.Hirata, C.Iwashita, A.Ochi, H.Hori
  • Organizer: 第1回モデル生物丸ごと一匹学会・第10回高度好熱菌連携会議
  • Place of Presentation: 兵庫県佐用郡
  • Year and Date: 2011-08-19
• [Presentation] The crystal structure analysis and establishment of activity mesurement system of Thermus thermophilus tRNA(Um34, Cm34) methyltransferase[YibK] (2011)
  • Author(s): T.Matsugi, A.Hirata, H.Hori
  • Organizer: 第1回モデル生物丸ごと一匹学会・第10回高度好熱菌連携会議
  • Place of Presentation: 兵庫県佐用郡
  • Year and Date: 2011-08-19
• [Presentation] Substrate tRNA recognition mechanism of FAD/folate-dependet tRNA m5U54 methyltransferase TrmFO (2011)
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-16
• [Presentation] Regulation of tRNA modification network by pseudouridine 55 in Thermus thermophiles (2011)
  • Author(s): C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-16
• [Presentation] A conserved Lys residue in Crenarchaea specific loop determines a broad substratespecificity of Crenarchaeal RNA splicing endonuclease (2011)
  • Author(s): A. Hirata, T. Kitajima, and H. Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-16
• [Presentation] Transfer RNA recognition mechanism of multi-site specific tRNA methyltransferase (Trm1) from Aquifex aeolicus (2011)
  • Author(s): T. Awai, A. Ochi, Ihsanawati, A. Hirata, Y. Bessho, S. Yokoyama, and H. Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: Kyoto
  • Year and Date: 2011-06-16
• [Presentation] Substrate tRNA recognition mechanism of FAD/folate-dependet tRNA m^5U54 methyltransferase TrmFO (2011)
  • Author(s): R.Yamagami, K.Yamashita, H.Nishimasu, C.Iwashita, A.Hirata, O.Nureki, H.Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 20-11Kyoto)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-16
• [Presentation] Regulation of tRNA modification network by pseudouridine 55 in Thermus thermophiles (2011)
  • Author(s): C.Tomikawa, K.Ishida, T.Kunibayashi, A.Ochi, T.Kanai, A.Hirata, C.Iwashita, H.Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-16
• [Presentation] A conserved Lys residue in Crenarchaea specific loop determines a broad substratespecificity of Crenarchaeal RNA splicing endonuclease (2011)
  • Author(s): A.Hirata, T.Kitajima, H.Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-16
• [Presentation] Transfer RNA recognition mechanism of multi-site specific tRNA methyltransferase (Trm1)from Aquifex aeolicus (2011)
  • Author(s): T.Awai, A.Ochi, Ihsanawati, A.Hirata, Y.Bessho, S.Yokoyama, H.Hori
  • Organizer: 16th Annual Meeting of RNA Society (RNA 2011Kyoto)
  • Place of Presentation: 京都
  • Year and Date: 2011-06-16
• [Presentation] アーキアにおける2'-5' RNA ligaseの構造機能解析 (2011)
  • Author(s): 長野倫子, 石丸雄基, 金井保, 平田章, 堀弘幸
  • Organizer: 第12回極限環境生物学会年会
  • Place of Presentation: 長崎
• [Presentation] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI) (2011)
  • Author(s): Hiroyuki Takuma, Masayuki Minoji, Natsumi Ushio, Chie Tomikawa, Akira Hirata, Anna Ochi, and Hiroyuki Hori
  • Organizer: The 38th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Sapporo
• [Presentation] Yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明
  • Author(s): 大森志穂、西田 裕、岡田和樹、平田 章、堀 弘幸
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 超好熱性アーキアThermococcus kodakarensis 2', 5'- RNA リガーゼの機能解析
  • Author(s): 平田 章、長野倫子、石丸雄基、金井 保、鈴木健夫、佐藤朝子、金井昭夫、鈴木 勉、堀 弘幸
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] Thermoplasma acidophilum由来tRNALeuにおいてArchaeosineが2カ所に形成されるメカニズムの解析
  • Author(s): 河村卓哉, 冨川千恵, 大平高之, 井上 仁, 行木信一, 岡 夏央, 谷口浩輝, 安藤香織, 大野 敏,山岸明彦, 鈴木 勉, 横川隆志, 堀 弘幸
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 真正細菌ジヒドロウリジン合成酵素はD20形成にtRNA上の他の修飾ヌクレオチドを必要とする
  • Author(s): 楠葉浩晃、吉田剛士、岩崎絵梨、粟井貴子、平田 章、冨川千恵、
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 熱ストレスによる開始tRNAMetの細胞内動態
  • Author(s): 渡邉和則、宮川 隆、冨川千恵、水野利恵、高橋昭久、大槻高史、堀弘幸、井尻憲一
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] m7G46およびΨ55を中心としたThermus thermophilus tRNA修飾ネットワーク
  • Author(s): 冨川千恵、石田一雄、国林貴史、金井 保、横川隆志、堀 弘幸
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 葉酸依存性tRNA(m5U54)メチル化酵素TrmFOはどうやってtRNA中のU54を認識しているのか?
  • Author(s): 山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田 章, 石谷隆一郎, 濡木 理, 堀 弘幸
  • Organizer: 第１５回日本RNA学会年会
  • Place of Presentation: 松山
• [Presentation] 固相化DNAプローブ法を用いた好熱菌tRNAの大量精製法の確立
  • Author(s): Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori
  • Organizer: 第３回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪
• [Presentation] Eukaryotic tRNA (m2G10) methyltransferase (Trm11-Trm112) recognizes the whole structure of tRNA
  • Author(s): Shiho Ohmori, Yu Nishida, Kazuki Okada, Akira Hirata, and Hiroyuki Hori
  • Organizer: 第３回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪
• [Presentation] Does D20 formation by eubacterial dihydrouridine synthase require the other modified nucleotides for its modification in tRNA?
  • Author(s): 楠葉浩晃、吉田剛士、岩崎絵梨、粟井貴子、平田 章、冨川千恵、山上龍太、堀 弘幸
  • Organizer: 第３回モデル生物丸ごと一匹学会年会
  • Place of Presentation: 大阪
• [Presentation] Thermococcus kodakarensis 由来tRNA メチル化酵素(Trm- m22G10)の構造機能解析
  • Author(s): 西山聖示、平田 章、田村俊浩、堀 弘幸
  • Organizer: 2013年度極限環境生物学会年会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] 超好熱性アーキア Thermococcus kodakarensis 由来 tRNATrp の
  • Author(s): 前田雄太、平田 章、長野倫子、土手智裕、鈴木健夫、金井 保、鈴木 勉、堀 弘幸
  • Organizer: 2013年度極限環境生物学会年会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] 高度好熱菌tRNA中のシュードウリジン55修飾は、温度変化への適応因子として機能する
  • Author(s): 冨川千恵，石田一雄，国林貴史，越智杏奈，金井 保，平田 章，岩下知香子，堀 弘幸
  • Organizer: 第１４回日本RNA学会年会
  • Place of Presentation: 仙台
• [Presentation] サーマス・サーモフィラス由来tRNA メチル化酵素TrmFOの基質認識機構
  • Author(s): 山上龍太，山下光輝，西増 弘志，冨川 千恵，越智 杏奈，岩下 智香子，平田 章，石谷隆一郎，濡木 理，堀 弘幸
  • Organizer: 第１４回日本RNA学会年会
  • Place of Presentation: 仙台
• [Presentation] tRNA modifications in Thermoplasma acidophilum
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 第１４回日本RNA学会年会
  • Place of Presentation: 仙台
• [Presentation] 極小アーキアにおけるtRNAスプライシングエンドヌクレアーゼの構造機能解析
  • Author(s): 平田 章，藤島皓介，山上龍太，河村卓哉，J. F. Banfield，堀 弘幸
  • Organizer: 第２５回日本アーキア研究会
  • Place of Presentation: 兵庫
• [Presentation] Structural insight into the evolution and broad substrate specificity of the fourth type of tRNA splicing endonuclease
  • Author(s): A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfiled, A. Kanai, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: スペイン
• [Presentation] Transfer RNA recognition mechanism of Thermus thermophilus folate/FAD-dependent tRNA methyltransferase (TrmFO)
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: スペイン
• [Presentation] Transfer RNA modifications in thermophilic bacteria
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 9th Extremophiles 2012
  • Place of Presentation: スペイン
• [Presentation] Adaptation of Thermus thermophilus to environmental temperature via tRNA modification enzymes-modified nucleotides network in tRNA
  • Author(s): C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Organizer: 第２回モデル生物丸ごと一匹学会
  • Place of Presentation: 兵庫
  • Invited
• [Presentation] Functional analysis of yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)
  • Author(s): Y. Nishida, K. Okada, A. Hirata, and H. Hori
  • Organizer: 第２回モデル生物丸ごと一匹学会
  • Place of Presentation: 兵庫
• [Presentation] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)
  • Author(s): H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori
  • Organizer: 第２回モデル生物丸ごと一匹学会
  • Place of Presentation: 兵庫
• [Presentation] Substrate transfer RNA recognition mechanism of tRNA (m5U54) methyltransferase (TrmFO) based on the biochemical and structural analysis
  • Author(s): R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori
  • Organizer: 第２回モデル生物丸ごと一匹学会
  • Place of Presentation: 兵庫
• [Presentation] RNAメチル化酵素の構造と反応機構の多様性：トポロジカル・ノット型RNAメチル化酵素発見からの８年間
  • Author(s): 堀 弘幸
  • Organizer: 愛媛大学第１８４回応用化学科セミナー
  • Place of Presentation: 松山
  • Invited
• [Presentation] RNAメチル化酵素の構造と反応機構の多様性
  • Author(s): 堀 弘幸
  • Organizer: 岐阜大学生命工学科セミナー
  • Place of Presentation: 岐阜
  • Invited
• [Presentation] Negative control of Gm18, m5s2U54 and m1A58 modifications by pseudouridine55 modification in Thermus thermophilus tRNAs: the tRNA modification network in extreme thermophile
  • Author(s): C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori
  • Organizer: 24th tRNA workshop
  • Place of Presentation: チリ
• [Presentation] Improvement of a protein purification method for nucleic acid-free in the case of recombinant tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) from Aquifex aeolicus
  • Author(s): S. Nishiyama, A. Hirata, T. Awai, and H. Hori
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Research for substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)
  • Author(s): H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Structural and functional analyses of archaeal 2’-5’ RNA ligase
  • Author(s): T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Expression and purification of yeast tRNA (Cm32/Gm34) methyltransferase (Trm7)
  • Author(s): K. Yoshii, A. Hirata, and H. Hori
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明
  • Author(s): 西田 裕、岡田和樹、平田 章、堀 弘幸
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] tRNA modifications in thermoacidophilic archaeon
  • Author(s): T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Book] 「生命分子を統合するRNA-その秘められた役割と制御機構」1-3「tRNA修飾の構造と機能」実験医学増刊号 (2013)
  • Author(s): 堀弘幸
  • Publisher: 羊土社
• [Book] 実験医学増刊号 生命分子を統合するRNA-その秘められた役割と制御機構1-3 tRNA修飾と機能 (2013)
  • Author(s): 堀 弘幸
  • Total Pages: 10
  • Publisher: 羊土社
• [Book] Degradation of hypo-modified tRNA in Thermus thermophilus, an extreme-thermophilic eubacteria, Advanced in Genetic Research, Vol. 6 (K. V. Urbano eds) (2011)
  • Author(s): C. Tomikawa and H. Hori
  • Publisher: Nova Science Publisher
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~achem/bchem/
• [Remarks] 応用生物化学研究室ＨＰ
  • URL: http://www.ehime-u.ac.jp/~achem/bchem/
• [Remarks] 愛媛大学工学部応用化学科応用生物化学研究室ホームページ
  • URL: http://www.ehime-u.ac.jp/~achem/bchem/
• [Remarks] 愛媛大学ホームページ研究活動トピックス
  • URL: http://www.ehime-u.ac.jp/research/news/detail.html?new_rec=9781
• [Remarks] 当研究室のホームページは下記URLです
  • URL: http://www.ehime-u.ac.jp/~achem/bchem/
• [Remarks] 随時、論文発表および学会発表の内容を広く全世界に公開しております