Next generation protein production on cellular quality-control process
| Project/Area Number |
23360371
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokushima |
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Principal Investigator |
OMASA Takeshi 徳島大学, ソシオテクノサイエンス研究部, 教授 (00252586)
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| Co-Investigator(Kenkyū-buntansha) |
ONITSUKA Masayoshi 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (80571174)
SHIRAI Akihiro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (40380117)
MASEDA Hideaki 徳島大学, 大学院・ソシオテクノサイエンス研究部, 准教授 (10372343)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 小胞体ストレス応答 / チャイニーズハムスター卵巣細胞 / 蛋白質医薬品 / 糖鎖 / 細胞培養 |
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| Research Abstract |
Therapeutic antibody is workhorse for the development of pharmaceutical industries. In Japan, more than quarter of biopharmaceuticals was produced using CHO cell line. Recently, over 10 g/L antibody production was reported using CHO cell line. In this project, we focused on the cellular quality-control system for protein secretion process in CHO cell. On the basis of quality-control mechanism, we constructed the improved protein production system.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α 2,6-sialytransferase derived from Chinese hamster ovary cells2012
Author(s)
Masayoshi Onitsuka, Wook-Dong Kim, Hiroyuki Ozaki, Akira Kawaguchi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutato Asano, Izumi Kumagai, Hisao Ohtake, Takeshi Omasa
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Journal Title
Applied Microbiology and Biotechnology
Volume: Vol. 94
Issue: 1
Pages: 69-80
DOI
Related Report
Peer Reviewed
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