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Quantitative and qualitative changes in dissolved organic matter and bacteria in Lake Biwa

Research Project

Project/Area Number 23370010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionKyoto University

Principal Investigator

NAKANO Shin-ichi  京都大学, 生態学研究センター, 教授 (50270723)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Kazuhide  滋賀県琵琶湖環境科学研究センター, 主任研究員 (80291178)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords溶存有機物 / 植物プランクトン / 浮遊細菌 / 化学組成 / 遺伝系統解析
Research Abstract

We have clarified the microbial processes below: In the epilimnion of Lake Biwa during the stratified period, phytoplankton produce protein-like dissolved organic matter (DOM), and the DOM is converted into humic-like DOM through decomposition by planktonic bacteria. The humic-like DOM is transported to the hypolimnion through winter vertical mixing. In the hypolimnion during the stratified period, only one bacterial species, CL500-11 (phylum Chloroflexi), predominates, and bacterivorous Kinetoplastid flagellates also dominate. So, in the hypolimnion of Lake Biwa during the stratified period, there may be the unique microbial food webs where humic-like DOM is consumed by bacterial assemblages predominated by CL500-11, and where those bacteria are grazed by bacterivorous protists such as Kinetoplastids.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (45 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (32 results) Book (2 results)

  • [Journal Article] Protistan grazing and viral lysis losses of bacterial carbon production in a large mesotrophic lake (Lake Biwa)2014

    • Author(s)
      Takasu, H., T. Kunihiro, S. Nakano
    • Journal Title

      Limnology

      Volume: (in press)

    • NAID

      120005477386

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 有機汚濁と環境基準-琵琶湖を例にして-2014

    • Author(s)
      津田久美子, 早川和秀, 岡本高弘
    • Journal Title

      用水と廃水

      Volume: 56(3) Pages: 59-72

    • NAID

      40020000011

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biogeochemical control on fluorescent dissolved organic matter dynamics in a large freshwater lake (Lake Biwa, Japan)2013

    • Author(s)
      Thottathil, S., K. Hayakawa, Y. Hodoki, C. Yoshimizu, Y. Kobayashi, S. Nakano
    • Journal Title

      Limnol. Oceanogr

      Volume: 58 Pages: 2262-2278

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] CARD-FISH analysis of prokaryotic community composition and abundance along small-scale vegetation gradients in a dry arctic tundra ecosystem2013

    • Author(s)
      Ushio, M., K. Makoto, J. Klaminder, S. Nakano
    • Journal Title

      Soil Biology & Biochemistry

      Volume: 64 Pages: 147-154

    • NAID

      120005293577

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of carbon biomass and community structure of planktonic bacteria in Lake Biwa using respiratory quinone analysis2013

    • Author(s)
      Takasu, H., T. Kunihiro and S. Nakano
    • Journal Title

      Limnology

      Volume: 14 Pages: 247-256

    • NAID

      10031202986

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal dominance of CL500-11 bacterioplankton (Phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa2013

    • Author(s)
      Okazaki, M., Y. Hodoki, S. Nakano
    • Journal Title

      Japan. FEMS Microbiol Ecol

      Volume: 83 Pages: 82-92

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal dominance of CL500-11 bacterioplankton (Phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan.2013

    • Author(s)
      Okazaki, M., Hodoki, Y. and Nakano, S.
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 83 Issue: 1 Pages: 82-92

    • DOI

      10.1111/j.1574-6941.2012.01451.x

    • NAID

      120005672537

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of carbon biomass and community structure of planktonic bacteria in Lake Biwa using respiratory quinone analysis2013

    • Author(s)
      Takasu, H., Kunihiro, T., Nakano, S.
    • Journal Title

      Limnology

      Volume: (印刷中)(掲載確定) Issue: 3 Pages: 247-256

    • DOI

      10.1007/s10201-013-0402-3

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CARD-FISH analysis of prokaryotic community composition and abundance along small-scale vegetation gradients in a dry arctic tundra ecosystem2013

    • Author(s)
      Ushio M, Makoto K, Klaminder J, Nakano S-I
    • Journal Title

      Soil Biology & Biochemistry

      Volume: 64 Pages: 147-154

    • DOI

      10.1016/j.soilbio.2013.05.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biogeochemical control on fluorescent dissolved organic matter dynamics in a large freshwater lake (Lake Biwa, Japan).2013

    • Author(s)
      Thottathil, S., K. Hayakawa, Y. Hodoki, C. Yoshimizu, Y. Kobayashi, S. Nakano
    • Journal Title

      Limnology and Oceanography

      Volume: 58 Issue: 6 Pages: 2262-2278

    • DOI

      10.4319/lo.2013.58.6.2262

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vertical community structure of bacteria and phytoplankton in Lake Biwa using respiratory quinone and pigment analysis2012

    • Author(s)
      Takasu, H., Kunihiro, T., Nakano, S.
    • Journal Title

      Interdisciplinary studies on environmental chemistry

      Volume: 6 Pages: 377-385

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bacterial community composition and enzyme activity from littoral zone to pelagic region in Lake Biwa2013

    • Author(s)
      Fujinaga, S., S. Nakano
    • Organizer
      "Big microbes" : International workshop on microbial ecology for young scientists
    • Place of Presentation
      大津市, 滋賀県
    • Year and Date
      2013-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 琵琶湖河口域から沖帯にかけての真菌現存量と酵素活性2013

    • Author(s)
      藤永承平・ 中野伸一
    • Organizer
      日本陸水学会第78回大津大会
    • Place of Presentation
      大津市, 滋賀県
    • Year and Date
      2013-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Seasonal changes in vertical abundance of Kinetoplastid nanoflagellates in Lake Biwa with special reference to ecological importance of a particular flagellate group2013

    • Author(s)
      Mukherjee, I., H. Nishino, H. Takasu, Y. Hodoki, S. Nakano
    • Organizer
      日本陸水学会第78回大津大会
    • Place of Presentation
      大津市, 滋賀県
    • Year and Date
      2013-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 静岡県佐鳴湖におけるピコプランクトン群集の増殖と原生生物の捕食による死滅2013

    • Author(s)
      高巣裕之・ Jessica E. LeClair・ 山本貴幸・谷幸則・ 中野伸一
    • Organizer
      日本陸水学会第78回大津大会
    • Place of Presentation
      大津市, 滋賀県
    • Year and Date
      2013-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Different land use patterns affect responses of river epilithic biofilms : A case study in Yasu River Watershed, Shiga, Japan2013

    • Author(s)
      Murakami, A., N. Okuda, T. Iwata, R. Williams, S. Nakano
    • Organizer
      The 13th Symposium on Aquatic Microbial Ecology
    • Place of Presentation
      Stresa, Italy
    • Year and Date
      2013-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Seasonal changes in vertical abundance of Kinetoplastid nanoflagellates in Lake Biwa with special reference to ecological importance of a particular flagellate group2013

    • Author(s)
      Mukherjee, I., H. Nishino, H. Takasu, Y. Hodoki, S. Nakano
    • Organizer
      第6回日本進化原生生物学研究会
    • Place of Presentation
      大阪市, 大阪府
    • Year and Date
      2013-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Seasonal changes in vertical abundance of Kinetoplastid nanoflagellates in Lake Biwa with special reference to ecological importance of a particular flagellate group2013

    • Author(s)
      Mukherjee, I., H. Nishino, H. Takasu, Y. Hodoki, S. Nakano
    • Organizer
      第6回日本進化原生生物学研究会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 琵琶湖河口域から沖帯にかけての真菌存在量と酵素活性2013

    • Author(s)
      藤永承平、中野伸一
    • Organizer
      日本陸水学会第78回大会
    • Place of Presentation
      滋賀県大津市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Seasonal changes in vertical abundance of Kinetoplastid nanoflagellates in Lake Biwa with special reference to ecological importance of a particular flagellate group2013

    • Author(s)
      Mukherjee, I., H. Nishino, H. Takasu, Y. Hodoki, S. Nakano
    • Organizer
      日本陸水学会第78回大会
    • Place of Presentation
      滋賀県大津市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 静岡県佐鳴湖におけるピコプランクトン群集の増殖と原生生物の捕食による死滅2013

    • Author(s)
      高巣裕之、Jessica E. LeClair、山本貴幸、谷幸則、中野伸一
    • Organizer
      日本陸水学会第78回大会
    • Place of Presentation
      滋賀県大津市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bacterial community composition and enzyme activity from littoral zone to pelagic region in Lake Biwa2013

    • Author(s)
      Fujinaga, S., S. Nakano
    • Organizer
      "Big microbes": International workshop on microbial ecology for young scientists
    • Place of Presentation
      滋賀県大津市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 呼吸鎖キノンを生物指標とした湖沼浮遊細菌の炭素バイオマス量および群集構造の評価2012

    • Author(s)
      高巣裕之・ 國弘忠生・ 中野伸一
    • Organizer
      日本陸水学会第77回名古屋大会
    • Place of Presentation
      名古屋市, 愛知県
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 琵琶湖水中の紫外吸収・蛍光物質の分布と特性の評価2012

    • Author(s)
      早川和秀・ 小島礼慈・ 和田千弦・ 杉山裕子・ 熊谷哲
    • Organizer
      日本陸水学会第77回名古屋大会
    • Place of Presentation
      名古屋市, 愛知県
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 琵琶湖の浮遊細菌群集の炭素現存量とその行方2012

    • Author(s)
      高巣裕之・ 國弘忠生・ 中野伸一
    • Organizer
      2012年度日本地球化学会年会
    • Place of Presentation
      博多市, 福岡県
    • Year and Date
      2012-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Biogeochemical control on fluorescent dissolved organic matter in a large freshwater lake (Lake Biwa, Japan)2012

    • Author(s)
      Thottathil, S., K. Hayakawa, Y. Hodoki, C. Yoshimizu, Y. Kobayashi, S. Nakano
    • Organizer
      Association for Sciences of Limnology and Oceanography Aquatic Sciences Meeting 2012
    • Place of Presentation
      コラボしが, 大津市, 滋賀県
    • Year and Date
      2012-07-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Carbon content and community structure of planktonic bacteria in large mesotrophic Lake Biwa using respiratory quinone analysis2012

    • Author(s)
      Takasu, H., T. Kunihiro, S. Nakano
    • Organizer
      Association for Sciences of Limnology and Oceanography Aquatic Sciences Meeting 2012
    • Place of Presentation
      コラボしが, 大津市, 滋賀県
    • Year and Date
      2012-07-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Long-term changes in the concentrations of nutrients and water quality parameters in Lake Biwa using integrated analysis from several monitoring data2012

    • Author(s)
      Hayakawa, K., S. Tsujimura, T. Ishikawa, H. Haga, T. Okamoto, C. Jiao, K. Ishikawa, M. Kumagai
    • Organizer
      Association for Sciences of Limnology and Oceanography Aquatic Sciences Meeting 2012
    • Place of Presentation
      コラボしが, 大津市, 滋賀県
    • Year and Date
      2012-07-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbial ecology of epilithic biofilms - Spatial and temporal positioning matters2012

    • Author(s)
      Murakami, A., S. Nakano
    • Organizer
      Association for Sciences of Limnology and Oceanography Aquatic Sciences Meeting 2012
    • Place of Presentation
      コラボしが, 大津市, 滋賀県
    • Year and Date
      2012-07-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Biogeochemical control on fluorescent dissolved organic matter in a large freshwater lake (Lake Biwa, Japan)2012

    • Author(s)
      Thottathil, D. S., Hayakawa, K., Hodoki, Y., Yoshimizu, C., Kobayashi, Y., Nakano, S.
    • Organizer
      ASLO Aquatic Science Meeting
    • Place of Presentation
      Otsu, Shiga, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Carbon content and community structure of planktonic bacteria in large mesotrophic Lake Biwa using respiratory quinone analysis2012

    • Author(s)
      Takasu, H., Kunihiro, T., Nakano, S.
    • Organizer
      ASLO Aquatic Science Meeting
    • Place of Presentation
      Otsu, Shiga, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Microbial ecology of epilithic biofilms - Spatial and temporal positioning matters2012

    • Author(s)
      Murakami, A., Nakano, S.
    • Organizer
      ASLO Aquatic Science Meeting
    • Place of Presentation
      Otsu, Shiga, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 呼吸鎖キノンを生物指標とした湖沼浮遊細菌の炭素バイオマス量および群集構造の評価2012

    • Author(s)
      高巣裕之、國弘忠生、中野伸一
    • Organizer
      日本陸水学会第77回名古屋大会
    • Place of Presentation
      愛知県、名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 琵琶湖の浮遊細菌群集の炭素現存量とその行方2012

    • Author(s)
      高巣裕之、國弘忠生、中野伸一
    • Organizer
      2012年度日本地球化学会年会
    • Place of Presentation
      福岡県、博多市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Long-term changes in the concentrations of nutrients and water quality parameters in Lake Biwa using integrated analysis from several monitoring data2012

    • Author(s)
      Hayakawa, K., Tsujimura, S., Ishikawa, T., Haga, H., Okamoto, T., Jiao, C., Ishikawa, K., Kumagai, M.
    • Organizer
      ASLO Aquatic Science Meeting
    • Place of Presentation
      Otsu, Shiga, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 琵琶湖深水層におけるCL500-11クラスター(クロロフレクサス門)に属する細菌の優占2011

    • Author(s)
      岡崎友輔、中野伸一
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 琵琶湖の表水層と深水層における細菌バイオマスの季節動態2011

    • Author(s)
      高巣裕之、國弘忠生、中野伸一
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 琵琶湖深層水におけるクロロフレクサス門細菌の優占2011

    • Author(s)
      岡崎友輔、中野伸一
    • Organizer
      日本陸水学会第76回大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 琵琶湖の表層水と深水層における細菌群集の動態-呼吸鎖キノンを生物指標とした評価-2011

    • Author(s)
      高巣裕之、國弘忠生、中野伸一
    • Organizer
      日本陸水学会第76回大会
    • Place of Presentation
      島根県松江市
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dominance of curve-shaped bacteria undetectable with conventional FISH probes in hypolimnion of Lake Biwa, Japan2011

    • Author(s)
      Okazaki, Y., S. Nakano
    • Organizer
      The 12th Symposium on Aquatic Microbial Ecology
    • Place of Presentation
      Rostock-Warnemunde, Germany
    • Year and Date
      2011-08-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Dominance of curve-shaped bacteria undetectable with conventional FISH probes in hypolimnion of Lake Biwa, Japan2011

    • Author(s)
      Okazaki, Y., Nakano, S.
    • Organizer
      The 12th Symposium on aquatic microbial ecology
    • Place of Presentation
      Rostock-Warnemunde, Germany
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Relative biomass of major phylogenetic bacterial groups and biological variables in mesotrophic Lake Biwa, Japan2011

    • Author(s)
      Takasu, H., Kunihiro, T., Nakano, S.
    • Organizer
      The 12th Symposium on aquatic microbial ecology
    • Place of Presentation
      Rostock-Warnemunde, Germany
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biomass of major phylogenetic bacterial groups in water column of large mesotrophic Lake Biwa, Japan2011

    • Author(s)
      Takasu, H., Kunihiro, T., Nakano, S.
    • Organizer
      International symposium on advanced studies by young scientists on environmental pollution and ecotoxicology
    • Place of Presentation
      愛媛県松山市
    • Year and Date
      2011-08-05
    • Related Report
      2011 Annual Research Report
  • [Book] Biodiversity and evolutionary research: from genome to ecosystem: Lake Biwa.2014

    • Author(s)
      Okuda, N., Watanabe, K., Fukumori, K., Nakano, S., Nakazawa, T.
    • Total Pages
      91
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] Biodiversity and evolutionary research: from genome to ecosystem

    • Author(s)
      Okuda, N., K. Watanabe, K. Fukumori, S. Nakano, T. Nakazawa
    • Publisher
      Lake Biwa
    • Related Report
      2013 Final Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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