Investigation of successive assembly mechanism of photosynthetic complexes
| Project/Area Number |
23370024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 光合成 / 葉緑体 / チラコイド膜 / 光化学系 / アンテナ / ステート遷移 / 酸素発生系 / キノン / 複合体 / サブユニット / 分子集合 / クラミドモナス / アンテナ複合体 / クロロフィルタンパク質 / 緑化 / 光合成色素 / クロロフィル / カロテノイド |
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| Outline of Final Research Achievements |
Photosynthetic reaction center and light-harvesting antenna form a complex consisting of a number of subunits and cofactors. In the present study, structure and successive assembly of these complexes has been analyzed using biochemical and molecular biological methods. The number and function of LHCII associated to PSI complex have been determined and the major naphthoquinone has been assigned as 5’-hydroxyphylloquinone. In addition, assembly mechanism of PSI complex has been analyzed using greening experiments and overexpressor of Ycf3 and Ycf4, which are required for PSI assembly. Site-directed mutagenesis that replaces amino acid residues involved in oxygen-evolution has been carried out to find that D1-N298 is an essential residue for the activity and protection against photodamage.
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Report
(4 results)
Research Products
(37 results)
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[Presentation] Biogenesis of Photosystem I complex2015
Author(s)
Nelaepalli Screedhar, Shin-ichiro Ozawa, Hidenori Matsuzaki, and Yuichiro Takahashi
Organizer
The German-Japanese Binational Seminar 2015
Place of Presentation
かんぽの宿熱海
Year and Date
2015-03-21 – 2015-03-26
Related Report
Invited
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