Insect growth-blocking peptide (GBP) signaling pathway mediates acute immune reactions during infectious and non-infectious stress

• Project/Area Number: 23370034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Saga University
• Principal Investigator: HAYAKAWA Yoichi 佐賀大学, 農学部, 教授 (50164926)
• Co-Investigator(Renkei-kenkyūsha): AIZAWA Tomoyasu 北海道大学, 大学院理学研究科, 准教授 (40333596)
• Research Collaborator: MATSUMOTO Hitoshi 佐賀大学, 農学部, 非常勤研究員 ; FURIHATA Shunsuke 鹿児島大学連合, 大学院農学研究科
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 昆虫 / サイトカイン / Growth blocking peptide / ストレス / 自然免疫 / 体液性免疫 / 細胞性免疫 / サイトカン / 非感染ストレス / セリン型プロテアーゼ / プロセシング / 血球 / growth-blocking peptide / Growth-blocking peptide (GBP) / 抗菌ペプチド / 温度 / ショウジョウバエ

Outline of Final Research Achievements

Antimicrobial peptides (AMPs), major innate immune effectors, are induced to protect hosts against invading microorganisms. AMPs are also induced under non-infectious stress; however, the signaling pathways of non-infectious stress-induced AMP expression are yet unclear. We demonstrated that GBP overexpression in Drosophila elevated expression of AMPs. GBP-induced AMP expression did not require Toll and immune deficiency (Imd) pathway-related genes, but imd and basket were essential, indicating that GBP signaling in Drosophila did not use the orthodox Toll or Imd pathway but used the JNK pathway after association with the adaptor protein Imd. The enhancement of AMP expression by non-infectious physical or environmental stressors was apparent in controls but not in GBP-knockdown larvae. These results indicate that the Drosophila GBP signaling pathway mediates acute innate immune reactions under various stresses, regardless of whether they are infectious or non-infectious.

Research Products

• [Journal Article] Immunoevasive protein (IEP)-containing surface layer covering polydnavirus particles is essential for viral infection. (2014)
  • Author(s): Furihata, S., Tanaka, K., Ryuda, M., Ochiai, M., Matsumoto, H., Csikos, G. and Hayakawa, Y.
  • Journal Title: J. Invertebr. Pathol. Volume: 115 Pages: 26-32
  • DOI: 10.1016/j.jip.2013.10.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gain of long tonic immobility behavioral trait causes the red flour beetle to reduce anti-stress capacity (2014)
  • Author(s): Kiyotake H, Matsumoto H, N akayama S, Sakai M, Miyata ke T, Ryuda M, and Hayakawa Y
  • Journal Title: Journal of Insect Physiology Volume: 60 Pages: 92-97
  • DOI: 10.1016/j.jinsphys.2013.11.008
  • Peer Reviewed
• [Journal Article] Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of the chalkbrood-infected colony after a pollination task in a greenhouse. (2014)
  • Author(s): Matsumoto, H., Nomura, S., Hayakawa, Y.
  • Journal Title: Applied Entomology and Zoology Volume: 49 Issue: 3 Pages: 395-402
  • DOI: 10.1007/s13355-014-0261-3
  • NAID: 40020169409
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stimulation of vitellogenin gene expression by permethrin in the brown planthopper, <i>Nilaparvata lugens</i> (Hemiptera: Auchenorrhyncha: Delphacidae) (2014)
  • Author(s): Khaeso, K., Matsumoto, H., Hayakawa, Y., Tojo, S.
  • Journal Title: Journal of Pesticide Science Volume: 39 Issue: 2 Pages: 98-104
  • DOI: 10.1584/jpestics.D13-052
  • NAID: 130004725729
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP. (2014)
  • Author(s): Tsuzuki, S. Matsumoto, H., Furihata, S., Ryuda, M., Tanaka, H., Sung, E-J., Bird, G.S., Zhou, Y., Shears, S.B., and Hayakawa, Y.,
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4628-4628
  • DOI: 10.1038/ncomms5628
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of the chalkbrood-infected colony after a pollination task in a greenhouse. (2014)
  • Author(s): Matsumoto, H., Nomura, S., Hayakawa, Y.
  • Journal Title: Applied Entomology and Zoology Volume: in press
  • NAID: 40020169409
  • Peer Reviewed
• [Journal Article] Vitellogenin gene expression by permethrin in the brown planthopper, Nilaparvata lugens (Hemiptera: Auchenorrhyncha: Delphacidae). (2014)
  • Author(s): Khaeso,K., Matsumoto, H., Hayakawa, Y., and Tojo, S.
  • Journal Title: J. Pesticide Sci. Volume: in press
  • Peer Reviewed
• [Journal Article] Venom components of Asobara japonica impair cellular immune responses of host Drosophila melanogaster (2013)
  • Author(s): Furihata,SX
  • Journal Title: Archives of Insect Biochemistry and Physiology Volume: 83 Issue: 2 Pages: 86-100
  • DOI: 10.1002/arch.21093
  • Peer Reviewed
• [Journal Article] Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress (2012)
  • Author(s): Tsuzuki, S., Ochiai, M., Matsumoto, H., Kurata, S., Ohnishi A., Hayakawa, Y.
  • Journal Title: Sci. Rep. Volume: 2 Issue: 1 Pages: 210-210
  • DOI: 10.1038/srep00210
  • Peer Reviewed
• [Journal Article] A stress-responsive cytokine-like gene regulates insect larval growth (2012)
  • Author(s): Yamaguchi, K., Nomura, S., Matsumoto, H., Tsuzuki, S., Hayakawa, Y.
  • Journal Title: Insect Biochem.Molec.Biol. Volume: 42 Issue: 3 Pages: 183-192
  • DOI: 10.1016/j.ibmb.2011.11.009
  • Peer Reviewed
• [Journal Article] Characteristics common to a cytokine family spanning five orders of insects (2012)
  • Author(s): Matsumoto, H., Tsuzuki, S., Date-Ito, A., Ohnishi, A., Hayakawa, Y.
  • Journal Title: Insect Biochem.Molec.Biol. Volume: 42 Issue: 6 Pages: 446-454
  • DOI: 10.1016/j.ibmb.2012.03.001
  • Peer Reviewed
• [Journal Article] Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signaling through ITPK1. (2012)
  • Author(s): Zhou, Y., Wu, S., Wang, H., Hayakawa, Y., Bird, G.S., and Shears, S.B.
  • Journal Title: Biochemical Journal Volume: 448 Issue: 2 Pages: 273-283
  • DOI: 10.1042/bj20120730
  • Peer Reviewed
• [Presentation] 宿主アワヨトウ幼虫からのカリヤコマユバチ脱出誘導因子の発見 (2014)
  • Author(s): 砂山貴志、内川雄貴、落合正則、早川洋一
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 高知大学
• [Presentation] 寄生蜂Asobara japonica毒液殺虫成分が宿主ショウジョウバエに及ぼす生理機能 (2013)
  • Author(s): 降幡駿介、早川洋一
  • Organizer: 第５７回日本応用動物昆虫学会大会
  • Place of Presentation: 日本大学生物資源科学科
• [Presentation] 昆虫自然免疫活性調節の新しい情報伝達経路 (2012)
  • Author(s): 早川洋一
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 近畿大学農学部(奈良)
  • Year and Date: 2012-03-28
• [Presentation] Biological functions of insect cytokine growth-blocking peptide (GBP) (2012)
  • Author(s): Yoichi Hayakawa
  • Organizer: International Congress of Entomology
  • Place of Presentation: Daegu, Korea
  • Invited
• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~hayakayo/FRAME/index.html
• [Patent(Industrial Property Rights)] ストレス耐性付与組成物、ストレス耐性付与方法、ストレス耐性体内増殖方法、及びストレス評価方法 (2015)
  • Inventor(s): 早川洋一
  • Industrial Property Rights Holder: 早川洋一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-059490
  • Filing Date: 2015-03-23