| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Research Abstract |
To identify victorin-binding proteins, a crude protein was extracted from the cell wall of the rice plant. The extract was fractionated by a gel filtration chromatography. Then, we have performed Biacore analysis to test if victorin binds to a cell wall protein. The Biacore positive fraction was mixed with the biotin-conjugated victorin (VicBio) and pulled down the victorin-binding proteins. A 15 kDa protein was specifically detected in VicBio-treated fraction, and mass spectrometry identified it as thioredoxin known as a reducing enzyme.
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