Molecular chemoecological study on the evolutionary process of pollination syndromes via floral synomone

• Project/Area Number: 23380035
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Kyoto University
• Principal Investigator: NISHIDA Ritsuo 京都大学, (連合)農学研究科(研究院), 教授 (30135545)
• Co-Investigator(Kenkyū-buntansha): ONO Hajime 京都大学, 大学院農学研究科, 助教 (70452282)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 送粉シンドローム / ミバエラン / フェニルプロパノイド / 共進化 / 化学受容 / 誘引物質 / 花香成分 / シノモン / 生合成 / フェロモン

Outline of Final Research Achievements

Phytophagous insects are highly adapted to plant secondary metabolites, whereas plants rely on insect pollinators often effectively guiding them by specific floral fragrances (synomones) in the mutualistic interactions. My research aims to understand the molecular basis of coevolutionary pathway in both pollinator insects (chemoreceptors to perceive floral volatiles) and flowering plants (floral synomone biosynthesis), particularly focusing on pollination syndromes between (1) Bactrocera fruit flies (Tephritidae:Diptera) and Bulbophyllum fruit fly orchids (Orchidacea), and (2) Desmometopa flies (Milichiidae:Diptera) and pipevine flowers (Aristolochia:Aristolochiaceae).

Research Products

• [Journal Article] Synonymization of key pest species within the Bactrocera dorsalis species complex (Diptera: Tephritidae): taxonomic changes based on 20 years of integrative morphological, molecular, cytogenetic, behavioral, and chemoecological data (2015)
  • Author(s): Schutze, M.K., Nishida, R., Ono, H., Clarke, A.R. et al.
  • Journal Title: Syst. Entomol. Volume: 40 Issue: 2 Pages: 456-471
  • DOI: 10.1111/syen.12113
  • Peer Reviewed
• [Journal Article] Stage-Specific Quercetin Sulfation in the Gut of Mythimna separata Larvae (Lepidoptera: Noctuidae) (2014)
  • Author(s): Aboshi T, Ishida M, Matsushita K, Hirano Y, Nishida R, Mori N.
  • Journal Title: Biosci Biotechnol Biochem, (in press) Volume: X Issue: 1 Pages: 38-40
  • DOI: 10.1080/09168451.2014.877835
  • Peer Reviewed
• [Journal Article] Chemical Ecology of Insect-Plant Interactions: Ecological Significance of Plant Secondary Metabolites (2014)
  • Author(s): Nishida, R
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 78
  • NAID: 120005646452
  • Peer Reviewed
• [Journal Article] Comparison of phenylpropanoid volatiles in male rectal pheromone gland after methyl eugenol consumption, and molecular phylogenetic relationship of four global pest fruit fly species-Bactrocera invadens, B.dorsalis, B.correcta and B.zonata (2011)
  • Author(s): Tan, K.H., Tokushima, I., Ono, H., Nishida, R.
  • Journal Title: Chemoecology Volume: 21 Issue: 1 Pages: 25-33
  • DOI: 10.1007/s00049-010-0063-1
  • Peer Reviewed
• [Journal Article] Larval feeding stimulants for a Rutaceae-feeding swallowtail butterfly, Papilio xuthus L.in Citrus unshiu leaves (2011)
  • Author(s): Murata, T., Mori, N., Nishida, R.
  • Journal Title: J.Chem.Ecol. Volume: 37 Issue: 10 Pages: 1099-1109
  • DOI: 10.1007/s10886-011-0022-5
  • Peer Reviewed
• [Journal Article] Conversion of 3-oxo steroids into ecdysteroids triggers molting and expression of 20E-inducible genes in Drosophila melanogaster (2010)
  • Author(s): Hajime Ono, Sayo Morita, Ichiyoh Asakura, Ritsuo Nishida
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 421 Issue: 3 Pages: 561-566
  • DOI: 10.1016/j.bbrc.2012.04.045
  • NAID: 120004161061
  • Peer Reviewed
• [Presentation] Ecological significance of plant secondary metabolites in insect-plant interactions (2015)
  • Author(s): Nishida, R.
  • Organizer: The 39th Conference of International Society of Chemical Ecology
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2015-06-29 – 2015-07-03
  • Invited
• [Presentation] 昆虫と植物をつなぐ化学因子：害虫ミバエ類の誘引戦略を中心として (2015)
  • Author(s): 西田律夫
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
  • Invited
• [Presentation] アゲハチョウ科食性進化の起源を探る:ウスバシロチョウの産卵刺激物質 (2015)
  • Author(s): 山口夕紀・小野肇・矢代敏久・西田律夫
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Bactrocera 属ミバエ類の化学感覚受容体の探索 (2015)
  • Author(s): 宮崎仁実・西田律夫・小野 肇
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] ナスミバエ誘引物質に対する雄成虫の羽化後日齢が誘引反応に与える影響および不妊虫と正常虫の誘引反応比較 (2015)
  • Author(s): 安藤緑樹・原口大・江口恭・宇久田理恵・西田律夫・小野肇・金城邦夫・佐渡山安常
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Search for New Fruit Fly Attractants from Plants (2014)
  • Author(s): Nishida, R., and Tan, K.H.
  • Organizer: 9th International Symposium on Fruit Flies of Economic Importance
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2014-05-12 – 2014-05-16
  • Invited
• [Presentation] ミバエラン Bulbophyllum macranthum の花香組成とミバエ誘引戦略 (2013)
  • Author(s): 西田律夫，中平昌孝，小野肇，Tan, K. H.
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 藤沢市
• [Presentation] ミカンコミバエ種群を誘引するBulbophyllum属ミバエランの花香成分 (2012)
  • Author(s): 西田律夫, Tan, K.H.
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 近畿大学農学部(奈良市)
  • Year and Date: 2012-03-29
• [Presentation] Phenylpropanoid bouquets of Bulbophyllum orchids act as floral synomones to attract Bactrocera fruit flies (2011)
  • Author(s): Nishida, R., Tan, K.H.
  • Organizer: Asia-Pacific Conference on Chemical Ecology
  • Place of Presentation: Beijing, China(招待講演)
  • Year and Date: 2011-10-13
• [Presentation] Synomonal fragrance of fruit fly orchids, Bulbophyllum macranthum and B. praetervisum, to attract fruit flies as pollinators
  • Author(s): Nishida, R., Nakahira, M., Ono, H., Wee, S.L., Tan, K.H.
  • Organizer: International Chemical Ecology Conference
  • Place of Presentation: Melbourne, Australia
• [Presentation] Synthesis and evaluation of oxygenated α-ionone derivatives as male attractants for the solanaceous fruit fly, Bactrocera latifrons (Diptera: Tephritidae)
  • Author(s): Eguchi, T., Yoshida, Y., Ukuda, R., Haraguchi, D., Kohama, T., Nishida, R.
  • Organizer: International Chemical Ecology Conference
  • Place of Presentation: Melbourne, Australia
• [Presentation] Chemical Ecology of Insect-Plant Interactions--Ecological Roles of Phytochemicals
  • Author(s): Ritsuo Nishida
  • Organizer: 日本化学会
  • Place of Presentation: 名古屋大学
  • Invited
• [Presentation] ウリミバエ類を送粉者とするミバエランの花香成分
  • Author(s): 勝手智子，小野肇，K.H. Tan，西田律夫
  • Organizer: 日本応用動物昆虫学会大会
  • Place of Presentation: 高知大学
• [Presentation] エノキに含まれるオオムラサキの産卵刺激物質
  • Author(s): 和田篤史，中出彩，小野肇，吉永直子，森直樹，西田律夫
  • Organizer: 日本農芸化学会2014年大会
  • Place of Presentation: 明治大学（生田キャンパス）
• [Presentation] 昆虫生理活性物質の化学生態学的研究
  • Author(s): 西田律夫
  • Organizer: 日本農芸化学
  • Place of Presentation: 仙台市
  • Invited
• [Book] Proceedings of the 9th International Symposium on Fruit Flies of Economic Importance (2015)
  • Author(s): Nishida, R. and Tan, K.H. （分担執筆）
  • Publisher: Ministry of Agriculture and Cooperatives of Thailand
• [Book] 植物色素フラボノイド (2013)
  • Author(s): 西田律夫（分担執筆）
  • Total Pages: 696
  • Publisher: 文一総合出版
• [Book] 昆虫フェロモンのバラエティー「化学受容の科学」(東原一成編) (2012)
  • Author(s): 西田律夫(分担執筆)
  • Publisher: 化学同人