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Making of multi-tolerant plants against complexed acid-soil stresses by molecular modifications of membrane lipid layers

Research Project

Project/Area Number 23380041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionYamagata University

Principal Investigator

WAGATSUMA Tadao  山形大学, 農学部, 客員教授 (70007079)

Co-Investigator(Kenkyū-buntansha) TAWARAYA Keitarou  山形大学, 農学部, 教授 (70179919)
WASAKI Jun  広島大学, 生物圏科学研究科, 准教授 (00374728)
WATANABE Toshihiro  北海道大学, (連合) 農学研究科 (研究院), 准教授 (60360939)
Co-Investigator(Renkei-kenkyūsha) KOYAMA Hiroyuki  岐阜大学, 応用生物科学部, 教授 (90234921)
TOYOMASU Tomonobu  山形大学, 農学部, 教授 (60272085)
KURODA Masaharu  独立行政法人農業, 食品産業技術総合研究機構・中央農業総合研究センター稲収量性研究北陸サブチーム, 主任研究員 (30355581)
OIKAWA Akira  山形大学, 農学部, 准教授 (50442934)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywordsアルミニウム耐性 / ガラクト脂質 / ステロール / 低リン耐性 / メタボローム / リン脂質 / リンリサイクリング能 / HMG遺伝子 / 代謝産物 / PAH遺伝子 / イネ / リンリサイクリング
Research Abstract

In order to lower the content of phospholipids and to increase that of sterols in roots, we made the new plants by modification using two different techniques, i.e., culturing method and molecular method: Al-sensitive rice cultivar Koshihikari grown previously under P-deficient conditions and transformed by the overexpressions of PAH (key gene to exchange phospholipids into galactolipids) and HMG (key gene to biosynthesize sterols). Al tolerance and low-Ca tolerance under low pH conditions of P-deficient Al-sensitive Koshihikari were significantly enhanced, and Al tolerance and low-P tolerance of the several lines of the transformed Koshihikari were also enhanced. Among several tens of rice cultivars, low-P tolerant rice cultivar was found out to have a specific characteristics on the decomposition of P-containing metabolites. Several genes contributing to P-recycling ability were identified together with each function.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (48 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (34 results) (of which Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Phosphorus deficiency enhances aluminum tolerance of rice (Oryza sativa) by changing the physicochemical characteristics of root plasma membranes and cell walls2014

    • Author(s)
      Maejima, E., Watanabe, T., Osaki, M. and Wagatsuma, T
    • Journal Title

      J Plant Physiol

      Volume: 171 Issue: 2 Pages: 9-15

    • DOI

      10.1016/j.jplph.2013.09.012

    • NAID

      120005411138

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and physiological analysis of Al3+ and H+ rhizotoxicities at moderately acidic conditions.2013

    • Author(s)
      Kobayashi, Y., Kobayashi, Y., Watanabe, T., Shaff J. E., Ohta, H., Kochian, L. V., Wagatsuma, T., Kinraide, T. B. & Koyama
    • Journal Title

      Plant Physiol

      Volume: 163 Issue: 1 Pages: 180-92

    • DOI

      10.1104/pp.113.222893

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice plant under phosphorus deficiency2013

    • Author(s)
      Tawaraya, K., Horie, R., Saito, A., Shinano, T., Wagatsuma, T., Saito, K., Oikawa, A
    • Journal Title

      J Plant Nutr

      Volume: 36 Issue: 7 Pages: 1138-1159

    • DOI

      10.1080/01904167.2013.780613

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 植物による難利用性リン酸利用のしくみと活用の可能性2013

    • Author(s)
      和崎 淳
    • Journal Title

      土づくりとエコ農業

      Volume: 45 Pages: 10-15

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Phytate degradation by fungi and bacteria that inhibit sawdust and coffee residue composts2013

    • Author(s)
      Eida, M. F.
    • Journal Title

      Microbes and Environments

      Volume: 28 Pages: 71-80

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン酸資源の枯渇に対応したリン栄養研究. 1.講座のねらい2012

    • Author(s)
      俵谷 圭太郎, 和崎 淳
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 173-176

    • NAID

      110009593696

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] リン酸資源の枯渇に対応したリン栄養研究 1.講座のねらい2012

    • Author(s)
      俵谷圭太郎
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 173-176

    • NAID

      110009593696

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リン酸資源の枯渇に対応したリン栄養研究 2.有機態リン酸の利用2012

    • Author(s)
      和崎淳
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 177-182

    • NAID

      110009593697

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の養分獲得・利用と施肥2012

    • Author(s)
      瀬戸二郎
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 83 Pages: 471-477

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of exogenous phosphatase and phytase activities on organic phosphate mobilization in soils with different phosphate adsorption capacities2012

    • Author(s)
      Maruyama, H., Yamamura, T., Kaneko, Y., Matsui, H., Watanabe, T., Shinano, T., Osaki, M., Wasaki, J.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 58 Issue: 1 Pages: 41-51

    • DOI

      10.1080/00380768.2012.656298

    • NAID

      110009594821

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of secreted proteins from aseptically grown rice2011

    • Author(s)
      Shinano, T.
    • Journal Title

      Phytochemistry

      Volume: 72(4-5) Issue: 4-5 Pages: 312-320

    • DOI

      10.1016/j.phytochem.2010.12.006

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] De novo RNA-seq と CE-TOF/MS による代謝産物プロファイリングに基づくシロバナルーピンの低リン耐性機構の解析2014

    • Author(s)
      丸山隼人、津村暁彦、海野佑介、及川彰、俵谷圭太郎、信濃卓郎、和崎淳
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学(富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞膜表面をターゲットとしたシロイヌナズナのアルミニウムストレスの解析2014

    • Author(s)
      小林安文、小林佑理子、渡部敏裕、我妻忠雄、Thomas B Kinraide、小山博之
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学(富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Phosphorus deficiency enhances the tolerance of rice to the acidic soil stress2013

    • Author(s)
      Maejima, E
    • Organizer
      XVII International Plant Nutrition Colloquium 2013
    • Place of Presentation
      Istanbul (Turkey)
    • Related Report
      2013 Final Research Report
  • [Presentation] HMG is a promising key gene for the enhancement of aluminum tolerance of rice via modulating membrane sterols in root-tip portion2013

    • Author(s)
      Wagatsuma, T
    • Organizer
      XVII International Plant Nutrition Colloquium 2013
    • Place of Presentation
      Istanbul (Turkey)
    • Related Report
      2013 Final Research Report
  • [Presentation] 強酸性土壌に生育する植物の根細胞膜成分の特性2013

    • Author(s)
      前島恵理子、渡部敏裕、我妻忠雄、大崎満
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Phosphorus deficiency enhances aluminum tolerance of rice to the acidic soil stress2013

    • Author(s)
      Maejima E, Watanabe T, Osaki M, Wagatsuma T
    • Organizer
      XVII International Plant Nutrition Colloquium
    • Place of Presentation
      ICEC (トルコ・イスタンブール)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of the phosphate acquisition ability and strategies of Arabidopsis thaliana accessions by analyzing root exudates2013

    • Author(s)
      Furutani A, Maruyama H, Okazaki K, Shinano T, Wasaki J
    • Organizer
      XVII International Plant Nutrition Colloquium
    • Place of Presentation
      ICEC (トルコ・イスタンブール)
    • Related Report
      2013 Annual Research Report
  • [Presentation] De novo sequencing and analysis of the white lupin root comparative transcriptome using a 454 GS Junior platform to discover genes involved in phosphorus deficiency2013

    • Author(s)
      Maruyama H, Unno Y, Shinano T, Wasaki J
    • Organizer
      XVII International Plant Nutrition Colloquium
    • Place of Presentation
      ICEC (トルコ・イスタンブール)
    • Related Report
      2013 Annual Research Report
  • [Presentation] HMG is a promising key gene for the enhancement of aluminum tolerance of rice via modulating membrane sterols in root-tip portion2013

    • Author(s)
      Wagatsuma T, Watanabe T, Toyomasu T, Kuroda M, Muranaka T, Ohyama K, Ishikawa A, Usui M, Maejima E, Khan MSH, Tawaraya K, Koyama H
    • Organizer
      XVII International Plant Nutrition Colloquium
    • Place of Presentation
      ICEC (トルコ・イスタンブール)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Lipidome profiling of phosphorus-deficient rice cultivars reveals remodeling of membrane lipids as a mechanisms of low P tolerance2013

    • Author(s)
      Tawaraya K, Yamazaki Y, Cheng W, Chuba M, Okazaki Y, Saito K, Oikawa A, Wagatsuma T
    • Organizer
      XVII International Plant Nutrition Colloquium
    • Place of Presentation
      ICEC (トルコ・イスタンブール)
    • Related Report
      2013 Annual Research Report
  • [Presentation] リン欠乏及びアルミニウム障害時におけるシロイヌナズナ野生株およびリンゴ酸分泌変異株の根浸出物の解析2012

    • Author(s)
      丸山隼人
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヤマモガシ科植物Hakea laurinaの低リン耐性における酸性ホスファターゼホモログの役割2012

    • Author(s)
      和崎淳
    • Organizer
      日本植物生理学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comparative transcriptomic analysis of P deficient plant roots2012

    • Author(s)
      Wasaki, J
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      Perth (Australia)
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの根脂質改変とアルミニウム耐性2012

    • Author(s)
      前島恵理子
    • Organizer
      日本土壌肥料学会本大会2012年度鳥取大会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 非菌根性シロバナルーピンの根圏におけるリン吸収能力とその活用2011

    • Author(s)
      和崎淳
    • Organizer
      菌根研究会 特別講演
    • Place of Presentation
      広島県東広島市(招待講演)
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phosphorus starvation changes the metabolic flow in rice2011

    • Author(s)
      Shinano, T.
    • Organizer
      3^<rd> International Symposium Frontiers in Agriculture Proteome Research-Contribution of proteomics technology in agricultural sciences
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-11-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mechanisms of adaptation of brachiariagrasses to abiotic stress factors in the tropics2011

    • Author(s)
      Rao, I.
    • Organizer
      III International Symposium on Forage Breeding
    • Place of Presentation
      Bonito, MS (Brazil)(招待講演)
    • Year and Date
      2011-11-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物機能の活用による土壌蓄積リンの効率的利用技術2011

    • Author(s)
      和崎淳
    • Organizer
      関東東海土壌肥料部会・秋季研究会
    • Place of Presentation
      埼玉県熊谷市(招待講演)
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of malate and citrate transporters induced in roots of phosphorus deficient Arabidopsis tbaliana2011

    • Author(s)
      Maruyama, H.
    • Organizer
      International Rhizosphere Conference 3
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plasma membrane sterols and the related gene in root-tip portion as key components for aluminum tolerance of varieties of plant species, cultivars, lines and mutants2011

    • Author(s)
      Wagatsuma, T.
    • Organizer
      International Rhizosphere Conference 3
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of phosphate utilization in Arabidopsis tbaliana natural accessions grown on soil2011

    • Author(s)
      Furutani, A.
    • Organizer
      International Rhizosphere Conference 3
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Metabolite profiling of phosphorus-deficient soybean root exudates2011

    • Author(s)
      Tawaraya, K.
    • Organizer
      International Rhizosphere Conference 3
    • Place of Presentation
      Perth (Australia)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Interactions between plants and phytate mineralizing bacteria isolated from the rhizosphere2011

    • Author(s)
      Mizukami, N.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌市
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物の養分ホメオスタシス(2)リン酸2011

    • Author(s)
      和崎淳
    • Organizer
      日本土壌肥料学会シンポジウム
    • Place of Presentation
      茨城県つくば市(招待講演)
    • Year and Date
      2011-08-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] リン欠乏シロイヌナズナ根からのリンゴ酸およびクエン酸分泌に関わるトランスポーターの解析2011

    • Author(s)
      丸山隼人
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of malate transporters induced in roots of phosphorus deficient or aluminum stressed Arabidopsis tbaliana2011

    • Author(s)
      Maruyama, H.
    • Organizer
      International Conference on Arabidopsis Research
    • Place of Presentation
      Madison, WI (USA)
    • Year and Date
      2011-06-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice under phosphorus deficiency

    • Author(s)
      Tawaraya, K
    • Organizer
      8th Symposium of the International Society of Root Research
    • Place of Presentation
      Dundee (UK)
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの根の細胞膜脂質改変とアルミニウムストレス耐性

    • Author(s)
      前島恵理子
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] The Challenge to Make Rice Plant Tolerant to Multi-stress in Acidic Soils

    • Author(s)
      Maejima, E.
    • Organizer
      The Fourth Hokkaido University Sustainability Research Poster Contest
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 難溶性リンの積極的獲得に関わる根分泌物質の輸送と制御

    • Author(s)
      和崎淳
    • Organizer
      岡山大学資源植物科学研究所 拠点共同研究主催ワークショップ 「植物ホルモンとイオン輸送体の相互理解」
    • Place of Presentation
      芸文館(倉敷市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ヤマモガシ科植物Hakea laurinaの低リン耐性機構の解析

    • Author(s)
      丸山隼人
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Comparative transcriptomic analysis of P deficient plant roots

    • Author(s)
      Wasaki, J.
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      マードック大学 (パース・オーストラリア)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Physiological responses of Hakea laurina (Proteaceae) to low-P conditions

    • Author(s)
      Maruyama, H.
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture IV
    • Place of Presentation
      マードック大学 (パース・オーストラリア)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Metabolite profiling of shoot extracts, root extracts, and root exudates of rice under phosphorus deficiency

    • Author(s)
      Tawaraya, K.
    • Organizer
      第8回国際根研究シンポジウム
    • Place of Presentation
      ダンディー (イギリス)
    • Related Report
      2012 Annual Research Report
  • [Book] Rijeka, Croatia. Physiological and biochemical mechanisms of plant adaptation to low-fertility acid soils of the tropics: The case of Brachiariagrasses. In Principles, Application and Assessment in Soil Science2011

    • Author(s)
      Watanabe, T., Khan, M.S.H., Rao, I.M., Wasaki, J., Shinano, T., Ishitani, M., Koyama, K., Ishikawa, S., Tawaraya, K., Nanamori, M., Ueki, N., Wagatsuma T
    • Publisher
      Intech
    • Related Report
      2013 Final Research Report
  • [Book] Principles, Application and Assessment in Soil Science2011

    • Author(s)
      Watanabe, T.
    • Total Pages
      30
    • Publisher
      InTech Open Access Publisher
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.geocities.jp/watanabe1209/Topics/topics.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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