Studies on olfactory memory molecules to natal stream odors in the brain of salmon

• Project/Area Number: 23380106
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: UEDA Hiroshi 北海道大学, 北方生物圏フィールド科学センター, 特任教授 (00160177)
• Co-Investigator(Kenkyū-buntansha): IIGO Masayuki 宇都宮大学, 農学部, 教授 (10232109) ; AMANO Masafumi 北里大学, 海洋生命科学部, 教授 (10296428) ; 渡辺 雅彦 北海道大学, 大学院・医学研究科, 教授 (70210945)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: サケ / 母川記銘 / 母川回帰 / 嗅覚記憶 / NMDA受容体 / 甲状腺刺激ホルモン放出ホルモン / 生殖腺刺激ホルモン放出ホルモン / 嗅電図 / 母川水ニオイ / TRH / GnRH / EOG / Y字水路 / 神経ホルモン

Outline of Final Research Achievements

Olfactory memory formation during juvenile salmon imprinting migration and retrieval during adult homing migration were investigated. In the brain of hatchery-reared underyearling juvenile chum salmon (Oncorhynchus keta), thyrotropin-releasing hormone (TRH) gene expression increased immediately after release from a hatchery into the natal steam, and the expression of the essential NR1 subunit of the N-methyl-D-aspartate receptor increased during downstream migration. Gene expression of salmon gonadotropin-releasing hormone (sGnRH) and NR1 increased in the adult chum salmon brain during homing from the Bering Sea to the natal hatchery. Thyroid hormone treatment in juveniles enhanced NR1 gene activation, and GnRHa treatment in adults improved stream odor discrimination. Olfactory memory formation during juvenile downstream migration and retrieval during adult homing migration of chum salmon might be controlled by endocrine hormones and could be clarified using NR1 as a molecular marker.

Research Products

• [Journal Article] Sex difference in metabolic rate and swimming performance in pink salmon (Oncorhynchus gorbuscha): the effect of male secondary sexual traits (2017)
  • Author(s): Makiguchi Y, Nii H, Nakao K, Ueda H
  • Journal Title: Ecology of Freshwater Fish Volume: 2016 Pages: 1-11
  • DOI: 10.1111/eff.12278
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Territories of heterologous inputs onto Purkinje cell dendrites are segregated by mGluR1-dependent parallel fiber synapse elimination. (2016)
  • Author(s): Ichikawa R, Hashimoto K, Miyazaki T, Uchigashima M, Yamasaki M, Aiba A, Kano M, Watanabe M
  • Journal Title: Sci Rep. Volume: 113 Pages: 21102-21102
  • DOI: 10.1038/srep21102
  • NAID: 120005828473
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] チューニングVPAを用いた洞爺湖産ヒメマスの資源評価 (2015)
  • Author(s): 蘇 宇, Emmanuel Andrew Sweke, 傳法 隆, 上田 宏, 松石 隆
  • Journal Title: 日本水産学会誌 Volume: 81 Pages: 413-428
  • NAID: 130005074185
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Catch per unit effort estimation and factors influencing it from recreational angling of sockeye salmon (Oncorhynchus nerka) and management implications for Lake Toya, Japan (2015)
  • Author(s): Sweke EA, Su y, Baba S, Denboh T, Ueda H, Sakurai Y, Matsuishi T
  • Journal Title: Lakes and Reservoirs: Research and Management Volume: 20 Pages: 264-274
  • NAID: 120005890028
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 7α-Hydroxypregnenolone, a key neuronal modulator of locomotion, stimulates upstream migration by means of the dopaminergic system in salmon (2015)
  • Author(s): Haraguchi S, Yamamoto Y, Suzuki Y, Hyung Chang J, Koyama T, Sato M, Mita M, Ueda H, Tsutsui K
  • Journal Title: Scientific Reports Volume: 5 Pages: 12546-12546
  • DOI: 10.1038/srep12546
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The olfactory transcriptome and progression of sexual maturation in homing chum salmon Oncorhynchus keta (2015)
  • Author(s): Palstra AP, Fukaya K, Chiba H, Dirks RP, Planas JV, Ueda H
  • Journal Title: PLoS ONE Volume: 10
  • DOI: 10.1371/journal.pone.0137404
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] サクラマススモルトの降下時における美利河ダム分水施設の評価 (2015)
  • Author(s): 林田寿文, 新居久也, 渡邉和好, 宮﨑俊行, 上田 宏
  • Journal Title: 土木学会論文集B1（水工学） Volume: 71
  • NAID: 130005123501
  • Peer Reviewed
• [Journal Article] The current propagation systems and physiological studies of Japanese chum salmon (2015)
  • Author(s): Ueda H
  • Journal Title: Fisheries Science Volume: 81 Pages: 219-228
  • DOI: 10.1007/s12562-014-0847-2
  • NAID: 40020416315
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 太平洋サケの母川記銘・回帰のメカニズム (2015)
  • Author(s): 上田 宏
  • Journal Title: ていち Volume: 127 Pages: 1-9
• [Journal Article] Comparison of the swimming ability and upstream-migration behavior between chum salmon and masu salmon (2014)
  • Author(s): Miyoshi K, Hayashida K, Sakashita T, Fujii M, Nii H, Nakao K and Ueda H
  • Journal Title: Canadian Journal of Fisheries and Aquatic Sciences Volume: 71 Pages: 217-225
  • DOI: 10.1139/cjfas-2013-0480
  • NAID: 120005425329
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cloning and characterization on the N-methyl-D-aspartate receptor subunit NR1 gene from chum salmon, Oncorhynchus keta (Walbaum, 1792) (2014)
  • Author(s): Yu JN, Ham SH, Lee SL, Jin HJ, Ueda H and Jin DH
  • Journal Title: Springer Plus Volume: 3 Pages: 9-9
  • DOI: 10.1186/2193-1801-3-9
  • Peer Reviewed
• [Journal Article] Effects of exogenous cortisol and seawater adaptation on thyroid hormone receptors in the smolt stage of the sockeye salmon, Oncorhynchus nerka (2014)
  • Author(s): Shin HS, Choi YJ, Kim NN, Lee J, Ueda H and Choi CY
  • Journal Title: Ichthyological Research Volume: 61 Pages: 9-16
  • DOI: 10.1007/s10228-013-0365-8
  • Peer Reviewed
• [Journal Article] Homing ability and migration success in Pacific salmon: mechanistic insights from biotelemetry, endocrinology, and neurophysiology (2014)
  • Author(s): Ueda H
  • Journal Title: Marine Ecology Progress Series Volume: 496 Pages: 219-232
  • DOI: 10.3354/meps10636
  • Peer Reviewed
• [Journal Article] Olfactory homing of chum salmon to stable compositions of amino acids in natal stream water (2013)
  • Author(s): Yamamoto Y, Shibata H and Ueda H
  • Journal Title: Zoological Science Volume: 30 Issue: 8 Pages: 607-607
  • DOI: 10.2108/zsj.30.607
  • NAID: 210000178084
  • ISSN: 0289-0003
  • Peer Reviewed
• [Journal Article] Diurnal changes in salmon GnRH secretion in the brain of masu salmon (Oncorhynchus masou) (2013)
  • Author(s): Fukaya, K., Amano, M., Ueda, H.
  • Journal Title: General and Comparative Endocrinology Volume: 192 Pages: 77-80
  • DOI: 10.1016/j.ygcen.2013.02.027
  • NAID: 120005350094
  • Peer Reviewed
• [Journal Article] Molecular evolution of Kiss2 genes and peptides in vertebrates (2013)
  • Author(s): T. Osugi, N. Ohtaki, Y. Sunakawa, Y. L. Son, M. Ohkubo, M. Iigo, M. Amano, K. Tsutsui
  • Journal Title: Endocrinology Volume: VOL.154 Pages: 4270-4280
  • DOI: 10.1210/en.2012-2267
  • Peer Reviewed
• [Journal Article] The saccus vasculosus of fish is a sensor of seasonal changes in day length (2013)
  • Author(s): Nakane, Y., Ikegami, K., Iigo, M., Ono, H., Takeda, K., Takahashi, D., Uesaka, M., Kimijima, M., Hashimoto, R., Arai, N., Suga, T., Kosuge, K., Abe, T., Maeda, R., Senga, T., Amiya, N., Azuma, T., Amano, M., Abe, H., Yamamoto, N., Yoshimura, T.
  • Journal Title: Nature Comm. Volume: 4, Article 2108 Pages: 2108-2108
  • DOI: 10.1038/ncomms3108
  • Peer Reviewed
• [Journal Article] pin hormone subunit mRNA in adult chum salmon (Oncorhynchus keta) (2013)
  • Author(s): Kim NN, Shin HS, Choi YJ, Yamamoto Y, Fukaya K, Ueda H and Choi CY
  • Journal Title: Marine and Freshwater Behaviour and Physiology Volume: 45 Pages: 397-410
  • DOI: 10.1080/10236244.2013.777215
  • Peer Reviewed
• [Journal Article] Expression of aquaporin-3 and -8 mRNAs in the parr and smolt stages of sockeye salmon, Oncorhynchus nerka: Effects of cortisol treatment and seawater acclimation (2013)
  • Author(s): Choi YJ, Shin HS, Kim NN, Cho SH, Yamamoto Y, Ueda H, Lee J and Choi CY
  • Journal Title: Comparative Biochemistry and Physiology, Part Volume: 165 Pages: 228-236
  • DOI: 10.1016/j.cbpa.2013.03.013
  • Peer Reviewed
• [Journal Article] Physiological mechanisms of imprinting and homing migration in Pacific salmon Oncorhynchus spp. (2012)
  • Author(s): Ueda H
  • Journal Title: Journal of Fish Biology Volume: 81 Pages: 543-558
  • DOI: 10.1111/j.1095-8649.2012.03370.x
  • Peer Reviewed
• [Journal Article] Changes in activity and transcript level of liver and gill metabolic enzymes during smoltification in wild and hatchery-reared masu salmon (Oncorhynchus masou) (2012)
  • Author(s): Mizuno S, Urabe H, Aoyama T, Omori H, Iijima A, Kasugai K, Torao M, Misaka N, Koide N and Ueda H
  • Journal Title: Aquaculture Volume: 362-363 Pages: 109-120
  • DOI: 10.1016/j.aquaculture.2010.10.034
  • NAID: 120004930532
  • Peer Reviewed
• [Journal Article] Effects of aggressive interaction on downstream movement and olfactory function in masu salmon, Oncorhynchus masou (2012)
  • Author(s): Lee SP and Ueda H
  • Journal Title: Aquaculture Volume: 362-363 Pages: 167-171
  • DOI: 10.1016/j.aquaculture.2010.09.012
  • NAID: 120004930533
  • Peer Reviewed
• [Journal Article] Physiological mechanisms of homing migration in Pacific salmon from behavioral to molecular biological approaches (2011)
  • Author(s): Ueda H
  • Journal Title: General and Comparative Endocrinology Volume: 170 Pages: 222-232
  • NAID: 120002725562
  • Peer Reviewed
• [Journal Article] Olfactory responses to natal stream water in sockeye salmon by BOLD fMRI (2011)
  • Author(s): Bandoh H, Kida I, Ueda H
  • Journal Title: PLoS ONE Volume: 6(1)
  • NAID: 120002725563
  • Peer Reviewed
• [Journal Article] EMG telemetry studies on upstream migration of chum salmon in the Toyohira river, Hokkaido, Japan (2011)
  • Author(s): Makiguchi M, Ueda H, 他6名7番目
  • Journal Title: Fish Physiology and Biochemistry Volume: 37 Pages: 273-284
  • DOI: 10.1007/s10695-011-9495-y
  • Peer Reviewed
• [Journal Article] 魚類の脳と匂い-サケの母川記銘・回帰機構 (2011)
  • Author(s): 上田宏
  • Journal Title: Aroma Research Volume: 46 Pages: 7-11
• [Journal Article] サケの母川記銘・回帰機構に関する生理学的研究 (2011)
  • Author(s): 上田宏
  • Journal Title: 比較内分泌学 Volume: 37 Pages: 5-13
  • NAID: 10027947418
• [Presentation] Physiological mechanism of imprinting and homing migration of Pacific salmon (2016)
  • Author(s): Ueda H
  • Organizer: 52nd Annual Meeting of Oregon Chapter of the American Fisheries Society
  • Place of Presentation: Seaside (USA)
  • Year and Date: 2016-03-04
  • Int'l Joint Research / Invited
• [Presentation] Physiological mechanism of imprinting and homing migration of Pacific salmon (2016)
  • Author(s): Ueda H
  • Organizer: Morioka Salmon Workshop
  • Place of Presentation: ホテルメトロポリタン盛岡ニューウイング（岩手県盛岡市）
  • Year and Date: 2016-02-08
  • Int'l Joint Research / Invited
• [Presentation] Physiological mechanisms of olfactory imprinting and homing in Pacific salmon (2015)
  • Author(s): Ueda H
  • Organizer: 145th Annual Meeting of American Fisheries Society
  • Place of Presentation: Portland (USA)
  • Year and Date: 2015-08-17
  • Int'l Joint Research
• [Presentation] Large and small scale Pacific salmon homing behavior using the PIT tag system (2015)
  • Author(s): Ueda H, Chen EY, Nii H, Nakao K, Hayashida K, Misaka N, Koide N
  • Organizer: 3rd International Conference on Fish Telemetry
  • Place of Presentation: Halifax (Canada)
  • Year and Date: 2015-07-16
  • Int'l Joint Research
• [Presentation] Neuroendocrine control mechanisms of imprinting and homing migration in chum salmon (2015)
  • Author(s): Ueda H
  • Organizer: International Symposium on Advances in the Population Ecology of Stream Salmonids IV
  • Place of Presentation: Girona (Spain)
  • Year and Date: 2015-05-26
  • Int'l Joint Research
• [Presentation] Neuroendocrine control mechanisms of imprinting and homing migration in Pacific salmon (2014)
  • Author(s): Ueda H
  • Organizer: International Symposium cum Workshop on Frontiers in Comparative Endocrinology and Neurobiology-2014
  • Place of Presentation: University of Hyderabad, India
  • Year and Date: 2014-11-27
  • Invited
• [Presentation] The current propagation system and physiological studies of imprinting and homing migration of Japanese chum salmon (2014)
  • Author(s): Ueda H
  • Organizer: 2nd FitFish Workshop on the Swimming Physiology of Fish
  • Place of Presentation: Institut d’Estudis Catalans (IEC), Barcelona, Spain
  • Year and Date: 2014-10-10
  • Invited
• [Presentation] Olfactory discriminating ability of adult chum salmon towards natal tributary streams (2014)
  • Author(s): Ueda H
  • Organizer: 11th International Congress on the Biology of Fish
  • Place of Presentation: Heriot-Watt University, Edinburg, England
  • Year and Date: 2014-08-06
• [Presentation] Physiological mechanism of olfactory imprinting and homing in Pacific salmon (2014)
  • Author(s): Ueda H
  • Organizer: The 2nd International Symposium on Frontier Science in Ecology, Genetics and Genomics
  • Place of Presentation: Faculty of Agriculture, Hokkaido University, Sapporo
  • Year and Date: 2014-07-10
  • Invited
• [Presentation] Neuroendocrine control mechanisms of imprinting and homing migration in Pacific salmon (2014)
  • Author(s): Ueda H
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao Municipal Auditorium, Olhao, Portugal
  • Year and Date: 2014-05-28
  • Invited
• [Presentation] Physiological mechanisms of olfactory imprinting and homing in salmon (2012)
  • Author(s): Ueda H
  • Organizer: 10th International Congress on the Biology of Fish
  • Place of Presentation: Monoa Terrace Community and Convention Center (USA)
  • Invited
• [Presentation] GnRH secretion patterns in Pacific salmon (2012)
  • Author(s): Fukaya K, Furukawa N, Murakami R, Amano M and Ueda H
  • Organizer: 7th International Symposium on Fish Endocrinology
  • Place of Presentation: NH City Hote (Argentina)
• [Presentation] 市民講座サケが生まれた川に帰る謎 (2011)
  • Author(s): 上田宏
  • Organizer: シンポジウム「沖縄における水棲動物生殖の基礎と応用」
  • Place of Presentation: 海洋博覧会記念公園総合研究センター(沖縄県国頭郡本部町)(招待講演)
  • Year and Date: 2011-12-10
• [Presentation] Mechanisms of imprinting and homing migration in salmon (2011)
  • Author(s): Ueda H
  • Organizer: American Fisheries Society 141st Annual Meeting, Symposium, Nature and Nurture
  • Place of Presentation: Washington State Convention Center(アメリカ合衆国)
  • Year and Date: 2011-09-08
• [Presentation] Neurophysiological mechanisms of olfactory imprinting and homing in salmon (2011)
  • Author(s): Ueda H
  • Organizer: 9th International Symposium on Fish Reproductive Physiology
  • Place of Presentation: Lulu International Convention Cente(インド共和国)(招待講演)
  • Year and Date: 2011-08-11
• [Presentation] Physiological mechanisms of imprinting and homing migration in salmon (2011)
  • Author(s): Ueda H
  • Organizer: 7th International Conference on Animal Navigation
  • Place of Presentation: University of Reading(イギリス）(招待講演)
  • Year and Date: 2011-04-08
• [Presentation] Physiological researches on imprinting and homing migration in Pacific salmon
  • Author(s): Ueda H
  • Organizer: 2nd International Conference on Fish Telemetry
  • Place of Presentation: Rhodes University (South Africa)
• [Presentation] 石狩川とサケ：河川改修とサケの遡河行動
  • Author(s): 上田 宏
  • Organizer: 第7回石狩川フォーラム
  • Place of Presentation: 紀伊國屋書店札幌本店（札幌）
  • Invited
• [Presentation] サケの母川回帰の不思議
  • Author(s): 上田 宏
  • Organizer: 平成25年度北海道大学水産学部公開講座
  • Place of Presentation: 北海道大学水産学部（函館）
  • Invited
• [Presentation] Olfactory imprinting during smoltification in Pacific salmon
  • Author(s): Ueda H
  • Organizer: 9th International Workshop on Salmonid Smoltification
  • Place of Presentation: Holar University College (Iceland)
  • Invited
• [Presentation] サケの嗅覚による母川記銘・回帰行動
  • Author(s): 上田 宏
  • Organizer: 日本学術会議公開シンポジウム「ここまで分かった水生動物行動の謎」
  • Place of Presentation: 日本学術会議（東京）
  • Invited
• [Presentation] Current status and future prospects of Pacific salmon propagation systems in Japan
  • Author(s): Ueda H
  • Organizer: Aquaculture 2013
  • Place of Presentation: Canarias Convention Centre (Gran Canaria)
• [Presentation] サケが川のニオイを嗅ぎ分ける行動
  • Author(s): 上田 宏
  • Organizer: 日本学術会議行動生物分科会サイエンスカフェ：個性の生物学
  • Place of Presentation: 東京大学駒場キャンパス（東京都）
  • Invited
• [Presentation] サケの母川回帰に及ぼす2種類のGnRHの役割
  • Author(s): 深谷厚輔・天野勝文・千葉洋明・Cheol Young Choi・上田宏
  • Organizer: 第6回サケ学研究会
  • Place of Presentation: 北海道大学水産学部（函館市）
• [Presentation] サケの母川回帰と河川環境
  • Author(s): 上田 宏
  • Organizer: 第20回日環協・環境セミナー全国大会in Hokkaid
  • Place of Presentation: 京王プラザホテル札幌（札幌市）
  • Invited
• [Book] 水産海洋ハンドブック第３版 (2016)
  • Author(s): 上田 宏
  • Total Pages: 662
  • Publisher: 生物研究者
• [Book] 三陸のサケ：復興のシンボル (2015)
  • Author(s): 上田 宏
  • Total Pages: 192
  • Publisher: 北海道大学出版会
• [Book] サケ学大全 (2013)
  • Author(s): 上田 宏
  • Total Pages: 296
  • Publisher: 北海道大学出版会
• [Book] Physiology and Ecology of Fish Migration (2013)
  • Author(s): Ueda H
  • Total Pages: 188
  • Publisher: CRC Press
• [Book] 行動生物学辞典 (2013)
  • Author(s): 上田 宏
  • Total Pages: 637
  • Publisher: 東京化学同人
• [Book] Sexual plasticity and gametogenesis in fishes (2013)
  • Author(s): Amano M
  • Total Pages: 256
  • Publisher: Nova Science Publishers
• [Book] Swimming Physiology of Fish (2012)
  • Author(s): Hayashida K, Fukaya K, Palstra AP and Ueda H
  • Total Pages: 429
  • Publisher: Springer-Verlag
• [Book] 森と海をむすぶ川 (2012)
  • Author(s): 柴田英昭，上田 宏，イレバ ニーナ，長尾誠也，中村洋平，門谷 茂，柴沼成一郎
  • Total Pages: 335
  • Publisher: 京都大学出版会
• [Book] The Biology of Odors (2011)
  • Author(s): Yamamoto Y, Bandoh H, Jin DH, Ueda H
  • Total Pages: 363
  • Publisher: Nova Science Publishers
• [Book] 川の蛇行復元 (2011)
  • Author(s): 牧口祐也, 新居久也, 中尾勝哉, 上田宏
  • Total Pages: 260
  • Publisher: 技報堂出版
• [Remarks] 上田研究室
  • URL: http://www.hucc.hokudai.ac.jp/~k15499/Ueda%20Laboratory%202013.html
• [Remarks] 上田研究室
  • URL: http://www.hucc.hokudai.ac.jp/~k15499/Ueda%20Laboratory%202013.html
• [Remarks] 上田研究室
  • URL: http://www.hucc.hokudai.ac.jp/~k15499/index.htm