Epigenetic analysis of zygotic gene activation by maternal factor
| Project/Area Number |
23380164
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
MINAMI Naojiro 京都大学, (連合)農学研究科(研究院), 准教授 (30212236)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938)
TSUKAMOTO Satoshi 放射線医学総合研究所, 主任研究員 (80510693)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | マウス初期胚 / エピジェネティクス / 胚性ゲノムの活性化 / 遺伝子発現 / 母性因子 / siRNA / 多能性 / 分化 / Smyd3 / Epigenetics / mouse embryo / development / Oct4 / Cdx2 / 受精卵 / ヒストンメチル化 / Chd1 / タンパク質分解 / ユビキチンプロテアソーム / リソソーム / マウス / 初期胚 / クロマチンリモデリング |
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| Outline of Final Research Achievements |
Smyd3 is a histone H3 lysine 4 (H3K4) di- and tri-methyltransferase that forms a transcriptional complex with RNA polymerase II and CHD1, which recognizes trimethylated histone H3 lysine 4, is a protein belonging to the family of ATPase-dependent chromatin remodeling factors. In the present study, we investigated the effects of RNA interference (RNAi)-mediated repression on the development of mouse embryos. In Smyd3-, and Chd1-knockdown embryos, the percentage of inner cell mass (ICM)-derived colony formation and trophectoderm (TE)-derived cell attachment was significantly decreased, resulting in a reduction in the number of viable offspring. Furthermore, the expression of Pou5f1, Nanog, and Cdx2, was dramatically decreased in both Smyd3- and Chd1-knockdown embryos. Moreover, in Chd1-knockdown embryos, expression of Hmgpi, which regulate Pou5f1, Nanog, and Cdx2, was also significantly suppressed at zygotic gene activation (ZGA).
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos2011
Author(s)
Miyamoto, K., Nagai, K., Kitamura, N., Nishikawa, T., Ikegami, H., Binh, N., Tsukamoto, S., Matsumoto, M., Tsukiyama, T., Minami, N., Yamada, M., Ariga, H., Miyake, M., Kawarasaki, T., Matsumoto, K., Imai, H.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 108
Issue: 17
Pages: 7040-7045
DOI
Related Report
Peer Reviewed
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