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Establishment of new environmental clean-up technique by used the plant-fungi-bacteria symbiosis

Research Project

Project/Area Number 23380190
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionIbaraki University

Principal Investigator

NARISAWA Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) OHTA Hiroyuki  茨城大学, 農学部, 教授 (80168947)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords共生 / エンドファイト / 菌類内生バクテリア / 環境浄化 / Cs吸収抑制 / 内生バクテリア / 根部エンドファイト / 生態
Research Abstract

We selected a species of the dark septate endophytic fungus (DSE), Veronaeopsis simplex, which promotes the growth of host plant even under the stressed conditions. Interestingly, the presence of bacterial communities living outside and inside mycelium of the fungus was revealed. We showed that the DSE has a complex interaction with associated bacteria involving host plant playing a role in growth promotion and environmental stress tolerance.
With regards to the Cs accumulation, it varied with the host plant considered. In Chinese cabbage, the DSE inoculation caused higher Cs accumulation in above ground plant parts, whereas in tomato, Cs accumulation decreased significantly with the isolate tested, suggesting low-risk transfer on the food chain as a result of high and negative plant reactions rather than high and positive reactions as it is the case with Chinese cabbage.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (47 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (32 results) (of which Invited: 1 results) Book (6 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Heteroconium chaetospira Induces Resistance to Clubroot via Upregulation of Host Genes Involved in Jasmonic Acid, Ethylene, and Auxin Biosynthesis.2014

    • Author(s)
      Lahlali R, McGregor L, Song T, Gossen BD, Narisawa K, and Peng G
    • Journal Title

      PLoS ONE

      Volume: 9(4) Issue: 4 Pages: e94144-e94144

    • DOI

      10.1371/journal.pone.0094144

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Control of Fusarium wilt in melon by the fungal endophyte, Cadophora sp2014

    • Author(s)
      Rida O. Khastini , T. Ogawara, Y. Sato and Narisawa K.
    • Journal Title

      Eur J Plant Pathol

      Volume: 139 Issue: 2 Pages: 333-342

    • DOI

      10.1007/s10658-014-0389-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Control of Fusarium wilt in melon by the fungal endophyte, Cadophora sp.2014

    • Author(s)
      Rida O. Khastini , T. Ogawara, Y. Sato and Narisawa K.
    • Journal Title

      Eur J Plant Pathol

      Volume: 139 Pages: 333-342

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new fungal endophyte, Scolecobasidiumhumicola, promoting growth of tomato under organic nitrogen conditions2013

    • Author(s)
      Rola S. Mahmoud and Narisawa, K
    • Journal Title

      PLoS ONE

      Volume: 8(11) Issue: 11 Pages: e78746-e78746

    • DOI

      10.1371/journal.pone.0078746

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] <i>Pseudosigmoidea ibarakiensis</i> sp. nov., a Dark Septate Endophytic Fungus from a Cedar Forest in Ibaraki, Japan2013

    • Author(s)
      Diene, O., Wang,W. T. and Narisawa, K
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 3 Pages: 381-387

    • DOI

      10.1264/jsme2.ME13002

    • NAID

      10031191422

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new fungal endophyte, Scolecobasidium humicola, promoting growth of tomato under organic nitrogen conditions.2013

    • Author(s)
      Rola S. Mahmoud and Narisawa, K
    • Journal Title

      PLoS ONE 8(11): e78746

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage2012

    • Author(s)
      Rida O. Khastini, H. Ohta and Narisawa K.
    • Journal Title

      THE JOURNAL OF MICROBIOLOGY

      Volume: Volume 50, Number 4 Issue: 4 Pages: 618-624

    • DOI

      10.1007/s12275-012-2105-6

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] スイートソルガム連作障害の原因解明とVeronaeopsis simplex Y34 を利用した同病害抑制について2013

    • Author(s)
      山本翔也、小松崎佑樹、塩津文隆、成澤才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 根部エンドファイト Veronaeopsis simplex Y34 による高温条件 下でのトマトの生育促進及び萎凋病の抑 制2013

    • Author(s)
      寺田晴菜, 成澤才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 根部エンドファイトPhialocephala fortinii を用いたアスパラガス育苗法の有効性2013

    • Author(s)
      松岡 勇人,菊池 聖永,畑 明李,成澤 才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Final Research Report
  • [Presentation] The fungal endophytes Cladophialophora sp. and Phialocephala helvetica which promote growth of tomato plant under salinity stressed conditions2013

    • Author(s)
      Erika Usui, Kazuhiko Narisawa
    • Organizer
      International Society for Environmental Biogeochemistry
    • Place of Presentation
      Huazhong Agricultural University, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Selection of Dark-septate endophytic (DSE) fungus associate with the roots of Spiranthes sinensis2013

    • Author(s)
      Saki Shingaki, Kazuhiko Narisawa
    • Organizer
      31st New Phytologist Symposium Orchid Symbiosis
    • Place of Presentation
      University of Calabria, Italy
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] スイートソルガム連作障害の原因解明とVeronaeopsis simplex Y34を利用した同病害抑制について2013

    • Author(s)
      山本翔也、小松崎佑樹、塩津文隆、成澤才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 根部エンドファイトVeronaeopsis simplex Y34による高温条件下でのトマトの生育促進及び萎凋病の抑制2013

    • Author(s)
      寺田晴菜, 成澤才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 根部エンドファイトPhialocephala fortiniiを用いたアスパラガス育苗法の有効性2013

    • Author(s)
      松岡 勇人,菊池 聖永,畑 明李,成 澤 才彦
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] The fungal endophytes Cladophialophora sp. and Phialocephala helvetica which promote growth of tomato plant under salinity stressed conditions2013

    • Author(s)
      Erika Usui, Kazuhiko Narisawa
    • Organizer
      International Society for Environmental Biogeochemistry
    • Place of Presentation
      Huazhong Agricultural University, China
    • Related Report
      2013 Annual Research Report
  • [Presentation] ピーマンへ耐塩性付与効果を示す根部エンドファイト2 種Phialocephala helvetica およびCladophialophora sp.について2012

    • Author(s)
      臼井絵里香, 成澤才彦
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Cunninghamella elegansに内生するBurkholderia 属細菌について2012

    • Author(s)
      高島勇介, 成澤才彦
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Mortierella 属菌の保有する内生細菌の地理的分布について2012

    • Author(s)
      親泊貴志,高島勇介,成澤才彦
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 低酸素条件における根部エンドファイトVeronaeopsis simplex Y34 株の植物生育促進効果2012

    • Author(s)
      小松崎佑樹, 成澤才彦
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] Colonization by the root endophytic fungus Camposporium indicum within roots of Rhododendron mucronatum2012

    • Author(s)
      皆川源一郎, 成澤才彦
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] ピーマンへ耐塩性付与効果を示す根部エンドファイト2種Phialocephala helveticaおよびCladophialophora sp.について2012

    • Author(s)
      臼井絵里香
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cunninghamella elegansに内生するBurkholderia属細菌について2012

    • Author(s)
      高島勇介
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mortierella属菌の保有する内生細菌の地理的分布について2012

    • Author(s)
      親泊貴志
    • Organizer
      日本菌学会第56回大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低酸素条件での利用に向けたDSEの獲得と選抜の試み2011

    • Author(s)
      小松崎佑樹、ワン・ウェイ, 成澤才彦
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-10-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 冷温帯落葉広葉樹林に生息するスギゴケからの菌類エンドファイトの分離と選抜2011

    • Author(s)
      皆川源一郎
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-10-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Change in the community of associated bacteria with Veronaeopsis simplex in the response of high temperature stress2011

    • Author(s)
      Rida Khastini, Yoshinori. Sato, Hiroyuki Ohta, Kazuhiko Narisawa
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      Kyoto Japan
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Change in the community of associated bacteria with Veronaeopsis simplex in the response of high temperature stress2011

    • Author(s)
      Rida Khastini, Yoshinori. Sato, Hiroyuki Ohta, Kazuhiko Narisawa
    • Organizer
      第27回日本微生物生態学会大会
    • Related Report
      2013 Final Research Report
  • [Presentation] Assessment of bacterial and fungal community structures in infected and non-infected field soil with Fusarium oxysporum2011

    • Author(s)
      Rola sameer Mahmoud,Kazuhiko Narisawa
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 低酸素条件での利用に向けたDSE の獲得と選抜の試み2011

    • Author(s)
      小松崎 佑樹、ワン・ウェイ、成澤 才彦
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物に塩類耐性を付与するDark-septate endophytic fungi (DSE)の選抜2011

    • Author(s)
      臼井 絵里香, 成澤 才彦
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 冷温帯落葉広葉樹林に生息するスギゴケからの菌類エンドファイトの分離と選抜2011

    • Author(s)
      皆川源一郎, 成澤 才彦
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Pythium aphanidermatum 及び P. nunn に内生する細菌様構造物の検出2011

    • Author(s)
      高島勇介, 佐藤嘉則, 東條元昭, 成澤才彦
    • Organizer
      第27回日本微生物生態学会大会
    • Place of Presentation
      京都大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Assessment of Veronaeopsis simplex in plant growth promotion and Fusarium disease suppression on Chinese cabbage in vitro2011

    • Author(s)
      Rida O. Khastini, Kazuhiko Narisawa, Hiroyuki Ohta
    • Organizer
      XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo Japan
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Invited
  • [Presentation] Assessment of bacterial and fungal community structures in infected and non-infected field soil with Fusarium oxysporum2011

    • Author(s)
      Rola sameer Mahmoud, Kazuhiko Narisawa
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      Kyoto university
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物に塩類耐性を付与するDark-septate endophytic fungi (DSE)の選抜2011

    • Author(s)
      臼井絵里香, 成澤才彦
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pythium aphanidermatum及びp.nunnに内生する細菌様構造物の検出2011

    • Author(s)
      高島勇介, 佐藤嘉則, 東條元昭, 成澤才彦
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 根部エンドファイト3種を利用したスィートソルガムによるCd汚染土壌浄化について2011

    • Author(s)
      河合由貴, 成澤才彦
    • Organizer
      日本微生物生態学会第27回大会
    • Place of Presentation
      京都大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of Fungal Communities associated with Mes ofauna in Low pH Forest Soils2011

    • Author(s)
      Anzilni Fathia Amasya, Rida Oktorida Khastini, Kazuhiko Narisawa, Makiko Watanabe
    • Organizer
      3rd Annual Argonne Soil Metagenomics Workshop
    • Place of Presentation
      Chicago, USA
    • Related Report
      2011 Annual Research Report
  • [Book] 土・植物・動物のつながりを探る科学 「農学入門」-食料・生命・環境科学の魅力-2013

    • Author(s)
      太田寛行、成澤才彦
    • Publisher
      養賢堂
    • Related Report
      2013 Final Research Report
  • [Book] エンドファイトの働きと使い方2011

    • Author(s)
      成澤才彦
    • Publisher
      農文協
    • Related Report
      2013 Final Research Report
  • [Book] Isolation and selection of fungal endophytes for the suppression of soil-borne diseases, In: A. M. Pirttila and S. Sorvari (Ed.), Prospects and Applications for Plant-Associated Microbes: A laboratory Manual2011

    • Author(s)
      Narisawa, K., and Diene, O.
    • Publisher
      BioBien Innovations, Paimio, Finland
    • Related Report
      2013 Final Research Report
  • [Book] Evaluation of fungal endophytes for the control of plant diseases, In: A. M. Pirttila and S. Sorvari (Eds.), Prospects and Applications for Plant-Associated Microbes: A laboratory Manual2011

    • Author(s)
      Narisawa, K., and Diene, O.
    • Publisher
      BioBien Innovations, Paimio, Finland
    • Related Report
      2013 Final Research Report
  • [Book] エンドファイトの働きと使い方2011

    • Author(s)
      成澤才彦
    • Total Pages
      117
    • Publisher
      社団法人 農山漁村文化協会
    • Related Report
      2011 Annual Research Report
  • [Book] Prospects and Applications for Plant-Associated Microbes : A laboratory Manual2011

    • Author(s)
      Narisawa, K., Diene, O.
    • Total Pages
      328
    • Publisher
      BioBien Innovations
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://info.ibaraki.ac.jp/scripts/websearch/index.htm

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 根部エンドファイト(DSE)Phialocephala for tiniiを利用したアスパラガス苗の生産方法2011

    • Inventor(s)
      成澤才彦、菊地聖永、竹下達夫
    • Industrial Property Rights Holder
      茨城大学、パイオニアエコサイエンス株式会社
    • Industrial Property Number
      2011-182330
    • Filing Date
      2011-09-16
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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