Elucidation of mechanisms governing selectable expression of chloroplast genes and its application to plant improvement
| Project/Area Number |
23380205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
KOBAYASHI Hirokazu 静岡県立大学, 大学院食品栄養環境科学研究院, 教授 (80170348)
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|---|
| Co-Investigator(Renkei-kenkyūsha) |
SHIMIZU Masanori 常葉大学, 健康プロデュース学部, 講師 (40468236)
KAWASHIMA Hiroto 静岡県立大学, 薬学部, 准教授 (50260336)
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|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | シグナル伝達 / 転写制御 / タンパク質リン酸化 / 葉緑体 / 植物 |
|---|
| Research Abstract |
Gene expression in chloroplasts was selectively switched off by the modification of sigma factor 1 (SIG1). We performed genome-wide screening of Arabidopsis cDNA clones by in vitro detection of protein-protein interaction, followed by phosphorylation assay, resulting in identification of some SIG-one protein kinases (SOPKs). Under lighting conditions that preferentially excited PS I, expression profiles of the psaA gene for the reaction center protein of PS I in SOPK-knockout (KO) lines, were consistent with SIG1 being in its unphosphorylated state. Employment of GFP fused with these SOPKs revealed their localization in the cytosol. SIG-one protein phosphatase (SOPH) candidate genes were tried to be identified by similar strategies. This "light switch" was further employed for chloroplast engineering. On the other hand, "gyokuro" green tea enriched with amino acids, is made of tea leaves grown under a light-shaded condition. Such leaves were subjected to transcriptomic analysis.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Establishment of a tea custom array based on EST data obtained with next-generation sequencing2013
Author(s)
Y. Niwa., S. Watanabe, T. Ogawa, H. Nagasaki, Y. Nakamura, H. Nishimura, T. Hirayama, E. Kobayashi, Y. Nakamura, H. Kobayashi
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Journal Title
Proceedings of "The 5th International Conference on O-CHA (Tea) Culture and Science, ICOS 2013"
Volume: PRP-34
Related Report
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[Journal Article] Establishment of a tea custom array based on EST data obtained with next-generation sequencing2013
Author(s)
Y. Niwa., S. Watanabe, T. Ogawa, H. Nagasaki, Y. Nakamura, H. Nishimura, T. Hirayama, E. Kobayashi, Y. Nakamura, and H. Kobayashi
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Journal Title
Proceedings of “The 5th International Conference on O-CHA (Tea) Culture and Science, ICOS 2013"
Volume: 2013
Related Report
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[Presentation] カスタムマイクロアレイを用いたチャ光制御遺伝子の発現解析2014
Author(s)
丹羽康夫, 西村秀希, 平山隆志, 澤田有司, 平井優美, 長崎英樹, 中村保一, 小林栄人, 渡辺祥子, 小川剛史, 中村順行, 小林裕和
Organizer
第55回日本植物生理学会年会
Place of Presentation
富山
Related Report
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[Presentation] カスタムマイクロアレイを用いたチャ光制御遺伝子の発現解析2014
Author(s)
丹羽康夫,西村秀希, 平山隆志, 澤田有司, 平井優美, 長崎英樹, 中村保一, 小林栄人, 渡辺祥子, 小川剛史, 中村順行, 小林裕和
Organizer
第55回 日本植物生理学会年会
Place of Presentation
富山
Related Report
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