Regulation of daily rhythms for behavior-metabolism links by light and nutrition

• Project/Area Number: 23390048
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Chiba University
• Principal Investigator: TAKIGUCHI Masaki 千葉大学, 医学(系)研究科(研究院), 教授 (40179578)
• Co-Investigator(Kenkyū-buntansha): IWASE Katsuro 千葉大学, 大学院医学研究院, 講師 (80322030)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Eriko 千葉大学, 大学院医学研究院, 技術職員 (20422256)
• Research Collaborator: ARITA Emiko 千葉大学, 大学院医学研究院, 技術職員 ; OOHIRA Ayano 千葉大学, 大学院医学研究院, 技術補佐員 ; TAMAI Keiko 千葉大学, 大学院医学研究院, 技術補佐員 ; TAIRA Akiko 千葉大学, 大学院医学研究院
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 生物時計 / 概日リズム / 糖新生 / 神経ペプチド / 時間生物学 / 行動学 / 時計遺伝子 / シグナル伝達 / 遺伝子発現制御 / 遺伝子 / 発現制御

Outline of Final Research Achievements

In the day/night cycle, mice act and eat during the dark period, while rest and fast during the light period. Under general conditions, lack of glucose activates gluconeogenetic enzyme genes in the latter half of the fasting period. However, depending on nutritional conditions, this rhythmicity suffers from fluctuations. Here, we revealed the fluctuation-associated changes in humoral factors including corticosterone, glucagon and insulin under the conditions of prolonged fasting, high-fat diet and carbohydrate/protein-ratio varied chows. A gene-disrupted mouse strain with a prolonged circadian behavioral rhythm under constant lightness showed an enhancing tendency in phase advancement of behavioral initiation by light pulse in the latter half of the dark period, and a shortening tendency in days required for resynchronization of the behavioral initiation phase after 6-h advancement of the dark period, suggesting the enhancement of light responsiveness of the daily behavioral rhythm.

Research Products

• [Journal Article] Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX) (2015)
  • Author(s): Machida, T., Kubota, M., Kobayashi, E., Iwadate, Y., Saeki, N., Yamaura, A., Nomura, F., Takiguchi, M., Hiwasa, T.
  • Journal Title: Journal of Translational Medicine Volume: 13 Issue: 1 Pages: 71-71
  • DOI: 10.1186/s12967-015-0393-4
  • Peer Reviewed / Open Access
• [Journal Article] Circulating anti-filamin C autoantibody as a potential serum biomarker for low-grade gliomas (2014)
  • Author(s): Adachi-Hayama, M., Adachi, A., Shinozaki, N., Matsutani, T., Hiwasa, T., Takiguchi, M., Saeki, N., Iwadate, Y.
  • Journal Title: BMC Cancer Volume: 14 Issue: 1 Pages: 452-452
  • DOI: 10.1186/1471-2407-14-452
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of cell growth by nuclear receptor COUP-TFI: Possible involvement of decorin in growth inhibition (2014)
  • Author(s): Morii, S., Kato, M., Seki, N., Shinmen, N., Iwase, K., Takiguchi, M., Hiwasa, T.
  • Journal Title: Biochemistry & Physiology Volume: S4
  • DOI: 10.4172/2168-9652.s4-001
  • Peer Reviewed / Open Access
• [Journal Article] The secretogranin II gene is a signal integrator of glutamate and dopamine inputs. (2014)
  • Author(s): Iwase, K., Ishihara, A., Yoshimura, S., Andoh, Y., Kato, M., Seki, N., Matsumoto, E., Hiwasa, T., Muller, D., Fukunaga, K., and Takiguchi, M.
  • Journal Title: Journal of Neurochemistry Volume: 128 Issue: 2 Pages: 233-245
  • DOI: 10.1111/jnc.12467
  • Peer Reviewed
• [Journal Article] Efficient subtractive cloning of genes activated by lipopolysaccharide and interferon γ in primary-cultured cortical cells of newborn mice. (2013)
  • Author(s): Miyauchi, O., Iwase, K., Itoh, K., Kato, M., Seki, N., Braissant, O., Bachmann, C., Shozu, M., Sekiya, S., Osada, H., and Takiguchi, M.
  • Journal Title: PLoS ONE Volume: 8 Issue: 11 Pages: e79236-e79236
  • DOI: 10.1371/journal.pone.0079236
  • Peer Reviewed
• [Journal Article] Autologous antibody to src-homology 3-domain GRB2-like 1 specifically increases in the sera of patients with low-grade gliomas (2012)
  • Author(s): 5. Matsutani T, Hiwasa T, Takiguchi M, Oide T, Kunimatsu M, Saeki N, Iwadate Y
  • Journal Title: J Exp Clin Cancer Res Volume: 31 Issue: 1 Pages: 31-85
  • DOI: 10.1186/1756-9966-31-85
  • Peer Reviewed
• [Journal Article] Calpain regulates thymidylate synthase-5-fluoro-dUMP complex levels associated with response to 5-fluorouracil in gastric cancer cells (2011)
  • Author(s): Nabeya, Y., Suzuki, T., Furuya, A., Koide, N., Ohkoshi, M., Takiguchi, M., Ochiai, T., Matsubara, H., Hiwasa, T.
  • Journal Title: Cancer Science Volume: 102 Issue: 8 Pages: 1509-1515
  • DOI: 10.1111/j.1349-7006.2011.01978.x
  • NAID: 10030242173
  • Peer Reviewed
• [Journal Article] Identification of a novel SEREX antigen family, ECSA, in esophageal squamous cell carcinoma (2011)
  • Author(s): Kagaya A, Shimada H, Shiratori T, et al.(2番目/17人中)
  • Journal Title: Proteome Science Volume: 9 Issue: 1 Pages: 31-31
  • DOI: 10.1186/1477-5956-9-31
  • Peer Reviewed
• [Journal Article] Functional similarity of anticancer drugs by MTT bioassay (2011)
  • Author(s): Hiwasa T, Iwadate Y, et al.
  • Journal Title: J Cancer Science & Therapy Volume: 3 Issue: 10 Pages: 250-255
  • DOI: 10.4172/1948-5956.1000099
  • Peer Reviewed