Development of novel cancer therapy using miRNA containing exosome
Project/Area Number |
23390087
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Tokyo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
OCHIYA Takahiro 独立行政法人国立がん研究センター, 研究所, 分野長 (60192530)
SUDO Katsuko 東京医科大学, 医学部, 兼任講師 (50126091)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
Keywords | エクソソーム / microRNA / EGFR / 分子標的治療 / miRNA / がん幹細胞 / 羊膜細胞 / iPS細胞 |
Research Abstract |
Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a microRNA to EGFR-expressing xenograft breast cancer tissue in Rag2-/- mice. Furthermore, we developed mimic miRNA that have efficient loading activity of the RISC complex. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.
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Report
(4 results)
Research Products
(129 results)
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[Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells.2014
Author(s)
Jeong-Hwan Yoon, Katsuko Sudo, Jin Soo Han, Min-Young Chae, Seok Hee Park, Michael Weinstein, Susumu Nakae, Tadashi Yamashita, In-Kyu Lee, Takayuki Sumida, Masahiko Kuroda, and Mizuko Mamura.
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Journal Title
European Journal of Immunology -2
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance2013
Author(s)
Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M, Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, Kurose H
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Journal Title
Nature Commun
Volume: 4
Issue: 1
Pages: 1532-1532
DOI
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Peer Reviewed
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[Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis2013
Author(s)
Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S and Kuroda M.
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Journal Title
BMC Cancer
Volume: 13
Issue: 1
Pages: 99-99
DOI
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Peer Reviewed
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[Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material.2013
Author(s)
Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
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Journal Title
Pathol Res Pract
Volume: 209
Issue: 7
Pages: 441-447
DOI
Related Report
Peer Reviewed
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[Journal Article] Classification of secondary corneal amyloidosis and involvement of lactoferrin.2013
Author(s)
Kaoru Araki-sasaki, Koji Hirano, Yasuhiro Osakabe, Masahiko Kuroda, Kazuko Kitagawa, Hiroshi Mishima, Hiroto Obata, Masakazu Yamada, Naoyuki Maeda, Kohji Nishida, Shigeru Kinoshita.
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Journal Title
Ophthalmology
Volume: 120
Issue: 6
Pages: 1166-1172
DOI
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Peer Reviewed
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[Journal Article] Efficacy of a novel class of RNA interference therapeutic agents2012
Author(s)
Hamasaki T, Suzuki H, Shirohzu H, Matsumoto T, D'Alessandro-Gabazza CN,Gil-Bernabe P, Boveda-Ruiz D, Naito M, Kobayashi T, Toda M, Mizutani T, TaguchiO, Morser J, Eguchi Y, Kuroda M, Ochiya T, Hayashi H, Gabazza EC, Ohgi T
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Journal Title
PLoS One
Volume: 7
Issue: 8
Pages: 1-15
DOI
Related Report
Peer Reviewed
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[Journal Article] TLS-CHOP represses miR-486 expression, inducing upregulation of a metastasis regulator PAI-1 in human myxoid liposarcoma2012
Author(s)
N. Borjigin, S. Ohno, W. Wu, M. Tanaka, R. Suzuki, K. Fujita, M. Takanashi, K. Oikawa, T. Goto, T. Motoi, T. Kosaka, K. Yamamoto, M. Kuroda
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 427
Issue: 2
Pages: 355-360
DOI
Related Report
Peer Reviewed
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[Presentation] 核酸医薬品の開発2014
Author(s)
黒田 雅彦
Organizer
日本がん分子標的治療学会
Place of Presentation
東京
Year and Date
2014-01-17
Related Report
Invited
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[Book] 細胞工学2013
Author(s)
大野慎一郎、髙梨 正勝、黒田 雅彦
Total Pages
3
Publisher
秀潤社
Related Report
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