Development of novel cancer therapy using miRNA containing exosome
Project/Area Number |
23390087
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human pathology
|
Research Institution | Tokyo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
OCHIYA Takahiro 独立行政法人国立がん研究センター, 研究所, 分野長 (60192530)
SUDO Katsuko 東京医科大学, 医学部, 兼任講師 (50126091)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
Keywords | エクソソーム / microRNA / EGFR / 分子標的治療 / miRNA / がん幹細胞 / 羊膜細胞 / iPS細胞 |
Research Abstract |
Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a microRNA to EGFR-expressing xenograft breast cancer tissue in Rag2-/- mice. Furthermore, we developed mimic miRNA that have efficient loading activity of the RISC complex. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.
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Report
(4 results)
Research Products
(139 results)
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[Journal Article] KLF5 regulates the integrity and oncogenicity of intestinal stem cells.2014
Author(s)
Nakaya T, Ogawa S, Manabe I, Tanaka M, Sanada M, Sato T, Taketo MM, Nakao K, Clevers H, Fukayama M, Kuroda M, Nagai R.
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Journal Title
Cancer Reseach
Volume: Epub ahead of print
Pages: in press
DOI
Related Report
Peer Reviewed
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[Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells.2014
Author(s)
Jeong-Hwan Yoon, Katsuko Sudo, Jin Soo Han, Min-Young Chae, Seok Hee Park, Michael Weinstein, Susumu Nakae, Tadashi Yamashita, In-Kyu Lee, Takayuki Sumida, Masahiko Kuroda, and Mizuko Mamura.
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Journal Title
European Journal of Immunology -2
Volume: in press
Pages: in press
DOI
Related Report
Peer Reviewed
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[Journal Article] Activin receptor-like kinase5 inhibition suppresses mouse melanoma by ubiquitin degradation of Smad4, thereby derepressing eomesodermin in cytotoxic T lymphocytes.2013
Author(s)
Jeong-Hwan Yoon, Su Myung Jung, Seok Hee Park, Mitsuyasu Kato, Tadashi Yamashita, In-Kyu Lee, Katsuko Sudo, Susumu Nakae, Jin Soo Han, Ok-Hee Kim, Byung-Chul Oh, Takayuki Sumida, Masahiko Kuroda, Ji-Hyeon Ju, Kyeong Cheon Jung, Seong Hoe Park, Dae-Kee Kim, and Mizuko Mamura.
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Journal Title
EMBO molecular Medicine.
Volume: 5
Pages: 1720-1739
DOI
Related Report
Peer Reviewed
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[Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material.2013
Author(s)
Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
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Journal Title
Pathol Res Pract
Volume: 209
Pages: 441-447
DOI
Related Report
Peer Reviewed
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[Journal Article] Classification of secondary corneal amyloidosis and involvement of lactoferrin.2013
Author(s)
Kaoru Araki-sasaki, Koji Hirano, Yasuhiro Osakabe, Masahiko Kuroda, Kazuko Kitagawa, Hiroshi Mishima, Hiroto Obata, Masakazu Yamada, Naoyuki Maeda, Kohji Nishida, Shigeru Kinoshita.
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Journal Title
Ophthalmology
Volume: 120
Pages: 1166-1172
DOI
Related Report
Peer Reviewed
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[Presentation] 核酸医薬品の開発2014
Author(s)
黒田 雅彦
Organizer
日本がん分子標的治療学会
Place of Presentation
東京
Year and Date
2014-01-17
Related Report
Invited
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[Presentation] miRNA医薬品の開発2013
Author(s)
黒田 雅彦
Organizer
第6回岐阜大学先端創薬医療シンポジウム
Place of Presentation
東京
Year and Date
20131129-20131129
Related Report
Invited
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[Book] 細胞工学2013
Author(s)
大野慎一郎、髙梨 正勝、黒田 雅彦
Total Pages
3
Publisher
秀潤社
Related Report
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