Molecular Basis of differentiation of Entamoeba histolytica

• Project/Area Number: 23390099
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: NOZAKI Tomoyoshi 国立感染症研究所, 寄生動物部, 部長 (60198588)
• Co-Investigator(Kenkyū-buntansha): TSUKUI Kumiko 国立感染症研究所, 寄生動物部, 主任研究官 (00420092) ; SATO Dan 慶應義塾大学, 先端生命科学研究所, 特別研究助教 (50468477)
• Co-Investigator(Renkei-kenkyūsha): JEELANI Ghulam (80725820)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 感染症 / 分化 / トランスクリプトーム / 寄生虫 / 病原性

Research Abstract

Metabolomics and transcriptomics analyses of encystation in Entamoeba invadens was conducted. We demonstrated a comprehensive view of metabolisms during encystation, including several unpredicted pathways and metabolites. These data should help us to further identify key regulatory points of gene expression and metabolic fluxes, and eventually understand elaborate regulation of entire metabolic network during encystation.

Research Products

• [Journal Article] Highly divergent mitochondrion-related organelles in anaerobic parasitic protozoa (2014)
  • Author(s): Makiuchi, T. and Nozaki, T
  • Journal Title: Biochimie Volume: 100 Pages: 3-17
• [Journal Article] Ligand heterogeneity of the cysteine protease binding protein family in the parasitic protist Entamoeba histolytica (2014)
  • Author(s): Marumo, K., Nakada-Tsukui, K., Tomii, K., and Nozaki, T
  • Journal Title: Int. J. Parasitol Volume: (in press)
• [Journal Article] Metabolomic analysis of Entamoeba : applications and implications (2014)
  • Author(s): Jeelani, G. and Nozaki, T
  • Journal Title: Curr. Opin. Microbiol Volume: (in press)
• [Journal Article] Cysteine protease-binding protein family 6 mediates the trafficking of amylases to phagosomes in the enteric protozoan Entamoeba histolytica (2013)
  • Author(s): Furukawa, A., Nakada-Tsukui, K., and Nozaki, T
  • Journal Title: Inf. Immun Volume: 81 Pages: 1820-1829
• [Journal Article] Iron sulfur clusters, their biosythesis and biological functions in protozoan parasites (2013)
  • Author(s): Ali, V. and Nozaki, T
  • Journal Title: Adv. Parasiol Volume: 83 Pages: 1-92
• [Journal Article] Transcriptome analysis of encystation in Entamoeba invadens (2013)
  • Author(s): Escueta- De Cadiz, A., Jeelani, G., Nakada-Tsukui, K., Caler, E., and Nozaki, T
  • Journal Title: PLoS One Volume: 8
• [Journal Article] Differential gene expression in Giardia lamblia under oxidative stress : Significance in eukaryotic evolution (2013)
  • Author(s): Raj, D., Ghosh, E., Mukherjee, A.K., Nozaki, T., and Ganguly, S
  • Journal Title: Gene Volume: (doi:pii:S0378-1119(13)01574-6)
• [Journal Article] ron sulfur clusters, their biosythesis and biological functions in protozoan parasites. Adv. Parasio (2013)
  • Author(s): Ali, V. and Nozaki, T
  • Journal Title: Adv. Parasiol Volume: 83 Pages: 1-92
  • DOI: 10.1016/b978-0-12-407705-8.00001-x
  • ISBN: 9780124077058
  • Peer Reviewed
• [Journal Article] Transcriptome analysis of encystation in Entamoeba invadens (2013)
  • Author(s): De Cadiz AE, Jeelani G, Nakada-Tsukui K, Caler E, Nozaki T
  • Journal Title: PLoS One Volume: 8 Issue: 9 Pages: e74840-e74840
  • DOI: 10.1371/journal.pone.0074840
  • Peer Reviewed
• [Journal Article] Differential gene expression in Giardia lamblia under oxidative stress: Significance in eukaryotic evolution. Gene (2013)
  • Author(s): Raj, D., Ghosh, E., Mukherjee, A.K., Nozaki, T., and Ganguly, S
  • Journal Title: Gene Volume: Dec 6 Issue: 2 Pages: 131-139
  • DOI: 10.1016/j.gene.2013.11.048
  • Peer Reviewed
• [Journal Article] Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica. Biochimie (2013)
  • Author(s): Jeelani, G., Husain, A., Sato, D., Soga, T., Suematsu, M., Nozaki, T
  • Journal Title: Biochimie Volume: 2013 95 Issue: 2 Pages: 309-319
  • DOI: 10.1016/j.biochi.2012.09.034
  • Peer Reviewed
• [Journal Article] Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica (2012)
  • Author(s): Penuliar, G. M., Furukawa, A., Nakada-Tsukui, K., Husain, A., Sato, D., and Nozaki, T
  • Journal Title: J. Antimicrob. Chemother Volume: 67 Pages: 375-386
• [Journal Article] Novel transmembrane receptor involved in phagosome transport of lysozymes and β-hexosaminidase in the enteric protozoan Entamoeba histolytica (2012)
  • Author(s): Furukawa, A., Nakada-Tsukui, K., and Nozaki, T
  • Journal Title: PLoS Pathog Volume: (doi:/journal.ppat.1002539)
• [Journal Article] Metabolic profiling of the protozoan parasite Entamoeba revealed activation of unpredicted pathway during encystation (2012)
  • Author(s): Jeelani, G,, Sato, S., Husain, A., Escueta-de Cadiza, A., Sugimoto, M., Soga, T., Suematsu, M., and Nozaki, T
  • Journal Title: PLoS ONE Volume: 7 Issue: 5 Pages: e37740-e37740
  • DOI: 10.1371/journal.pone.0037740
  • Peer Reviewed
• [Journal Article] Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica (2012)
  • Author(s): Husain, A., Sato, D., Jeelani, G., Soga, T., and Nozaki, T
  • Journal Title: PLoS Negl. Trop. Dis Volume: 6 Issue: 9 Pages: e1831-e1831
  • DOI: 10.1371/journal.pntd.0001831
  • Peer Reviewed
• [Journal Article] Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba.histolytica (2012)
  • Author(s): Penuliar, G.M., Furukawa, A., Nakada-Tsukui, K, Husain, A., Sato, D., Nozaki, T.
  • Journal Title: J.Antimicrob.Chemother. Volume: 67 Pages: 375-386
  • Peer Reviewed
• [Journal Article] Sulfate activation in mitosomes plays a crucial role in the proliferation of Entamoeba histolytica (2012)
  • Author(s): Furukawa, A., Nakada-Tsukui, K., Nozaki, T.Novel transmembrane receptor involved in phagosome transport of lysozymes and ss-hexosaminidase in the enteric protozoan Entamoeba histolytica. (2) :. doi : /journal.ppat.1002539, 2012
  • Journal Title: PLoS Pathogens Volume: 8
  • Peer Reviewed
• [Journal Article] Global Analysis of gene expression in response to L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica (2011)
  • Author(s): Husain, A., Jeelani, G., Sato, D., and Nozaki, T
  • Journal Title: BMC Genomics Volume: 12 Pages: 275-275
• [Journal Article] Sulfate activation in mitosomes plays a crucial role in the proliferation of Entamoeba histolytica (2011)
  • Author(s): Mi-ichi, F., Makiuchi, T., Furukawa, A., Sato, D., and Nozaki, T
  • Journal Title: PLoS Negl. Trop. Dis Volume: 5
• [Journal Article] Amebiasis in HIV-1-infected Japanese men : clinical features and response to therapy (2011)
  • Author(s): Watanabe, K., Gatanaga, H., de Cadiz, A.E., Tanuma, J., Nozaki, T., Oka, S
  • Journal Title: PLoS Negl. Trop. Dis Volume: 5
• [Journal Article] Mechanism of trifluoromethionine resistance in Entamoeba histolytica (2011)
  • Author(s): Penuliar, G. M., Furukawa, A., Sato, D., and Nozaki, T
  • Journal Title: J. Antimicrob. Chemother Volume: 66 Pages: 2045-2052
• [Journal Article] Global Analysis of gene expression in response to L-cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica (2011)
  • Author(s): Husain, A., Jeelani, G., Sato, D., Nozaki, T.
  • Journal Title: BMC Genomics Volume: 12 Pages: 275-275
  • Peer Reviewed
• [Journal Article] Sulfate activation in mitosomes plays a crucial role in the proliferation of Entamoeba histolytica (2011)
  • Author(s): Mi-ichi, F., Makiuchi, T., Furukawa, A., Sato, D., Nozaki, T.
  • Journal Title: PLoS Negl.Trop.Dis. Volume: 5
  • Peer Reviewed
• [Journal Article] Amebiasis in HIV-1-Infected Japanese Men : Clinical Features and Response to Therapy (2011)
  • Author(s): Watanabe, K., Gatanaga, H., de Cadiz, A.E., Tanuma, J., Nozaki, T., Oka, S.
  • Journal Title: PLoS Negl.Trop.Dis.
  • Peer Reviewed
• [Journal Article] Mechanism of trifluoromethionine resistance in Entamoeba histolytica (2011)
  • Author(s): Penuliar, G.M., Furukawa, A., Sato, D., Nozaki.T.
  • Journal Title: J.Antimicrob.Chemother. Volume: 66 Pages: 2045-2052
  • Peer Reviewed
• [Presentation] Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica (2013)
  • Author(s): Jeelani, G., Husain, A., Sato, D., Soga, T., Suematsu, M., Nozaki, T
• [Presentation] Metabolic profiling of the protozoan parasite Entamoeba revealed activation of unpredicted pathway during encystation (2012)
  • Author(s): Jeelani, G, , Sato, S., Husain, A., Escueta-de Cadiza, A., Sugimoto, M., Soga, T., Suematsu, M., and Nozaki, T
• [Presentation] Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica (2012)
  • Author(s): Husain, A., Sato, D., Jeelani, G., Soga, T., and Nozaki, T
• [Presentation] 赤痢アメーバが L- システイン欠乏下で特異的に賛成するアミノ酸とリン脂質の同定, 及びその生合成経路の解明 (2012)
  • Author(s): 佐藤暖 , Afzal Husain, Ghulam Jeelani, 曽我朋義 , 野崎智義
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: Nishinomiya, Hyogo
• [Presentation] Entamoeba invadens における安定発現株の樹立 (2012)
  • Author(s): 間瀬望, 津久井久美子, 野崎智義
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: Nishinomiya, Hyogo
• [Presentation] Metabolome of encystation in Entamoeba histolytica (2012)
  • Author(s): Jeelani, G., Dan, S., Husain, A., Escueta-de Cadiz, Sugimoto, M., Soga, T., Suematsu, M., and Nozaki, T
  • Organizer: Metabolomics of Protozoan parasites
  • Place of Presentation: Glasgow, U.K.
• [Presentation] Organelle metabolomics of Entamoeba histolytica (2012)
  • Author(s): Chiba, Y., Dan, S., Makiuchi, T., Jeelani, G., Soga, T., and Nozaki, T
  • Organizer: Metabolomics of Protozoan Parasites
  • Place of Presentation: Glasgow, U.K.
• [Presentation] A novel class of hydrolase receptors involved in the lysosomal trafficking of virulence factors in Entamoeba histolytica (2011)
  • Author(s): Nozaki, T
  • Organizer: The Second Cross-Straits and Asia Pacific International Conference on Parasites
  • Place of Presentation: Tainan, Taiwan
  • Invited
• [Presentation] A novel import machinery of the highly divergent mitochondrion-related organelle from the anaerobic parasitic protist Entamoeba histolytica (2011)
  • Author(s): Nozaki, T
  • Organizer: International Union of Microbiological Societies
  • Place of Presentation: Sapporo, Japan. (Symposist and Convenor)
• [Presentation] Function and transport of the highly divergent mitochondrion-related organelle from the anaerobic parasitic protist Entamoeba histolytica (2011)
  • Author(s): Nozaki, T
  • Organizer: The 1st Asian Conference of Prosistology
  • Place of Presentation: Jeju, Korea
• [Presentation] A novel class of hydrolase receptors involved in the lysosomal trafficking of virulence factors in Entamoeba histolytica (2011)
  • Author(s): Nozaki, T
  • Organizer: Joint International Tropical Medicine Meeting
  • Place of Presentation: Bangkok, Thailand
• [Presentation] A novel import machinery of the highly divergent mitochondrion-related organelle from the anaerobic parasitic protist Entamoeba histolytica (2011)
  • Author(s): Nozaki, T.
  • Organizer: International Union of Microbiological Societies
  • Place of Presentation: Sapporo, Japan
• [Remarks] 国立感染症研究所 寄生動物部
  • URL: http://www.nih.go.jp/niid/ja/from-para.html
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/2011-05-26-00-03-52.html