Study on the development of molecules for depressing neuropathic pain based on tertiary structure of human P2X4 receptor
| Project/Area Number |
23390155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kyushu University |
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Principal Investigator |
UEDA Tadashi 九州大学, 薬学研究科(研究院), 教授 (90184928)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Yoshito 九州大学, 大学院・薬学研究院, 准教授 (60315091)
TANAKA Hiroyuki 九州大学, 大学院・薬学研究院, 准教授 (30253470)
SHIROISH Mitsunori 九州大学, 大学院・薬学研究院, 助教 (00380527)
SAITO Hidetoshi 九州大学, 大学院・薬学研究院, 助教 (90444794)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Kazuhide 九州大学, 大学院・薬学研究院, 教授 (80124379)
TSUDA Makoto 九州大学, 大学院・薬学研究院, 准教授 (40373394)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 抗体 / 神経障害性疼痛 / 蛋白質工学 / P2X4受容体 / in Silicoスクリーニング |
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| Research Abstract |
We planed to develop molecules for depressing neuropathic pain involved in P2X4 receptor. Since we prepared a monoclonal antibody bound to rat P2X4 extracellular region, we planed to prepare monoclonal antibodies bound to human P2X4 extracellular regions using similar methods. Although a couple of types of monoclonal antibodies bound to human P2X4 extracellular regions were prepared, they did not have a potential to depress neuropathic pain. We newly developed the method to evaluate the interaction of a monoclonal antibody with a functionally trimeric P2X4 receptor. Using the method, it was shown that prepared monoclonal antibodies prepared in this study did not bind to a functionally trimeric P2X4 receptor, which may be the reason why monoclonal antibodies bound to human P2X4 extracellular regions did not have a potential to depress neuropathic pain.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor.2013
Author(s)
Igawa, T., Higashi, S., Abe, Y., Ohkuri, T., Tanaka, H., Morimoto, S., Yamashita, T., Tsuda, M., Inoue K., Ueda, T.
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Journal Title
Journal of Biochemistry,
Volume: 153(3)
Issue: 3
Pages: 275-82
DOI
Related Report
Peer Reviewed
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[Presentation] 抗ラット P2X4 抗体の抗原認識機構2011
Author(s)
井川達弘, 阿部義人, 東 貞行, 白石充典, 津田誠, 田中宏幸, 井上和秀, 植田正
Organizer
第11回日本蛋白質科学会
Place of Presentation
ホテル阪急エキスポパーク(吹田市)
Year and Date
2011-06-07
Related Report
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