Establishment of therapeutic lymphangiogenesis for lymphedema
Project/Area Number |
23390208
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Circulatory organs internal medicine
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Research Institution | Nagoya University |
Principal Investigator |
MUROHARA TOYOAKI 名古屋大学, 医学(系)研究科(研究院), 教授 (90299503)
|
Co-Investigator(Kenkyū-buntansha) |
SHINTANI Satoshi 名古屋大学, 医学系研究科, 講師 (20309777)
SHIBATA Rei 名古屋大学, 医学系研究科, 特任講師 (70343689)
|
Co-Investigator(Renkei-kenkyūsha) |
KONDO Kazuhisa 名古屋大学, 医学部附属病院, 病院助教 (90644659)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 再生医学 / リンパ浮腫 / リンパ管再生 / リンパ管内皮細胞 / 細胞移植療法 / 間葉系幹細胞 / 脂肪組織由来幹細胞 / サイトカイン / リンパ管 / アディポネクチン / リンパ管再生療法 / 再生医療 / 細胞治療 / 血管新生 / 成長因子 / 脂肪組織由来再生細胞 / マクロファージ |
Research Abstract |
Lymphedema is a serious clinical problem that occurs after surgical resection of cancers. We report that implantation of adipose-derived regenerative cells (ADRCs) can induce lymphangiogenesis in a mouse model of lymphedema. ADRCs were isolated from C57BL/6J mice. To examine the efficacy of ADRC implantation in vivo, we established a new mouse model of tail lymphedema. Lymphedema was improved significantly by local injection of ADRCs. Histological analysis revealed that lymphatic capillary density was greater in the ADRC group than in the PBS-injected control group. Tissue expression of vascular endothelial growth factor C (VEGF-C) was greater in the ADRC group than in the control group. ADRCs released VEGF-C, which stimulated lymphangiogenesis. Implantation of ADRCs also enhanced recruitment of M2 macrophages, which served as lymphatic endothelial progenitors. In conclusion, implantation of ADRCs could be a useful treatment option for lymphedema by mediation of lymphangiogenesis.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Adiponectin-mediated modulation of lymphatic vessel formation and lymphedema2013
Author(s)
Shimizu Y, Shibata R, Ishii M, Ohashi K, Kambara T, Uemura Y, Yuasa D, Kataoka Y, Kihara S, Murohara T, Ouchi N
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Journal Title
J. Am. Heart Assoc
Volume: 2
Related Report
Peer Reviewed
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[Journal Article] Therapeutic reendothelialization by induced pluripotent stem cells after vascular injury--brief report2013
Author(s)
Yamamoto T, Shibata R, Ishii M, Kanemura N, Kito T, Suzuki H, Miyake H, Maeda K, Tanigawa T, Ouchi N, Murohara T
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Journal Title
Arterioscler. Thromb. Vasc. Biol
Volume: 33
Pages: 2218-2221
Related Report
Peer Reviewed
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[Journal Article] iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis.2013
Author(s)
Kito T, Shibata R, Ishii M, Suzuki H, Himeno T, Kataoka Y, Yamamura Y, Yamamoto T, Nishio N, Ito S, Numaguchi Y, Tanigawa T, Yamashita JK, Ouchi N, Honda H, Isobe K, Murohara T.
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Journal Title
Sci Rep
Volume: 11:3
Issue: 1
Pages: 1418-1418
DOI
Related Report
Peer Reviewed
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[Journal Article] Adipose-derived factor ctrp9 attenuates vascular smooth muscle cell proliferation and neointimal formation2013
Author(s)
Uemura Y, Shibata R, Ohashi K, Enomoto T, Kambara T, Yamamoto T, Ogura Y, Yuasa D, Joki Y, Matsuo K, Miyabe M, Kataoka Y, Murohara T, Ouchi N
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Journal Title
FASEB J
Volume: 27
Pages: 25-33
Related Report
Peer Reviewed
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[Journal Article] Adiponectin-mediated modulation of lymphatic vessel formation and lymphedema2013
Author(s)
Shimizu Y, Shibata R, Ishii M, Ohashi K, Kambara T, Uemura Y, Yuasa D, Kataoka Y, Kihara S, Murohara T, Ouchi N
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Journal Title
J Am Heart Assoc
Volume: 2
Issue: 5
DOI
Related Report
Peer Reviewed
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[Journal Article] Therapeutic Reendothelialization by Induced Pluripotent Stem Cells After Vascular Injury2013
Author(s)
Yamamoto T, Shibata R, Ishii M, Kanemura N, Kito T, Suzuki H, Miyake H, Maeda K, Tanigawa T, Ouchi N, Murohara T
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 33
Issue: 9
Pages: 2218-21
DOI
Related Report
Peer Reviewed
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[Journal Article] Stress augments insulin resistance and prothrombotic state : Role of visceral adipose-derived monocyte chemoattractant protein-12012
Author(s)
Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T
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Journal Title
Diabetes
Volume: 61
Pages: 1552-1561
Related Report
Peer Reviewed
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[Journal Article] Therapeutic impact of Follistatin-like 1 on myocardial ischemic injury in preclinical animal models2012
Author(s)
Ogura Y, Ouchi N, Ohashi K, Shibata R, Kataoka Y, Kambara T, Kito T, Maruyama S, Yuasa D, Matsuo K, Enomoto T, Uemura Y, Miyabe M, Ishii M, Yamamoto T, Shimizu Y, Walsh K, Murohara T
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Journal Title
Circulation
Volume: 126
Pages: 1728-1738
Related Report
Peer Reviewed
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[Journal Article] Fat-derived factor omentin stimulates endothelial cell function and ischemia-induced revascularization via an endothelial nitric oxide synthase-dependent mechanism2012
Author(s)
Maruyama S, Shibata R, Kikuchi R, Izumiya Y, Rokutanda T, Araki S, Kataoka Y, Ohashi K, Daida H, Kihara S, Ogawa H, Murohara T, Ouchi N
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Journal Title
J Biol Chem
Volume: 287
Issue: 1
Pages: 408-17
DOI
Related Report
Peer Reviewed
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[Journal Article] The actin-binding protein girdin and its akt-mediated phosphorylation regulate neointima formation after vascular injury2011
Author(s)
Miyake H, Maeda K, Asai N, Shibata R, Ichimiya H, Isotani-Sakakibara M, Yamamura Y, Kato K, Enomoto A, Takahashi M, Murohara T
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Journal Title
Circ. Res
Volume: 108
Pages: 1170-1179
Related Report
Peer Reviewed
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[Presentation] C1q/Tnf Related Protein9 Prevents Lps Induced Acute Cardiac Injury Through An Ampk Dependent Mechanism2013
Author(s)
Takahiro Kambara, Rei Shibata, Satoko Hayakawa, Takashi Enomoto, Yusuke Uemura, Megumi Miyabe, Yoshiyuki Kataoka, Koji Ohashi, Noriyuki Ouchi, Toyoaki Murohara,
Organizer
American Heart Association 2013
Place of Presentation
Dallas Convention Center, Dallas, Texas USA
Related Report
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[Presentation] The Adipokine Omentin Prevents the Pathological Vascular Remodeling2013
Author(s)
Yusuke Uemura, Rei Shibata, Koji Ohashi, Takashi Enomoto, Megumi Miyabe, Yoshiyuki Kataoka, Takahiro Kambara, Kazuhiro Matsuo, Daisuke Yuasa, Yusuke Joki, Satoko Hayakawa, Mizuho Ito, Toyoaki Murohara, Noriyuki Ouchi,
Organizer
American Heart Association 2013
Place of Presentation
Dallas Convention Center, Dallas, Texas USA
Related Report
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