Elucidation of transcriptional and epigenomic regulation in the development of heart failure

• Project/Area Number: 23390210
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: KUWAHARA Koichiro 京都大学, 医学(系)研究科(研究院), 講師 (30402887)
• Co-Investigator(Kenkyū-buntansha): NISHIKIMI Toshio 京都大学, 大学院・医学研究科, 准教授 (80291946) ; NAKAGAWA Yasuaki 京都大学, 大学院・医学研究科, 助教 (70452357) ; KINOSHITA Hideyuki 京都大学, 大学院・医学研究科, 助教 (30467477)
• Co-Investigator(Renkei-kenkyūsha): TAKANO Makoto 自治医科大学, 医学部, 教授 (30236252) ; ARAI Yuji 独立行政法人国立循環器病研究センター, 分子生物学部, 室長 (30202724) ; MORI Yasuo 京都大学, 工学研究科, 教授 (80212265) ; NISHIDA Motohiro 九州大学, 薬学研究科, 准教授 (90342641)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 転写調節 / 心不全 / 転写 / エピゲノム / 遺伝子 / 転写因子 / 遺伝子発現 / イオンチャネル

Research Abstract

We examined the transcriptional and epigenomic regulation of cardiac gene expression during the development of heart failure. We found that NRSF-HDAC complex maintains cardiac electrostability by regulating HCN2 and 4 ion channels expression. In addition, we revealed the important role played by MRTF-A, a rho-dependent co-activator of SRF, in pathological cardiac remodeling. Furthermore, we analyzed the therapeutic effect of TRPC3/6 inhibition on pathological cardiac remodeling.

Research Products

• [Journal Article] The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis (2014)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K
  • Journal Title: PLoS One Volume: 9(3)
  • NAID: 120007100132
• [Journal Article] The effects of super-flux (high performance) dialyzer on plasma glycosylated pro-B-type natriuretic peptide (proBNP) and glycosylated N-Terminal proBNP in end-stage renal disease patients on dialysis. (2014)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K.
  • Journal Title: PLoS One Volume: 25 Issue: 3 Pages: e92314-e92314
  • DOI: 10.1371/journal.pone.0092314
  • NAID: 120007100132
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Chemicals Inducing Cardiomyocyte Proliferation in Developmental Stage-Specific Manner with Pluripotent Stem Cells (2013)
  • Author(s): Uosaki H, Magadum A, Seo K, Fukushima H, Takeuchi A, Nakagawa Y, Moyes KW, Narazaki G, Kuwahara K, Laflamme M, Matsuoka S, Nakatsuji N, Nakao K, Kwon C, Kass DA, Engel FB, Yamashita JK
  • Journal Title: Circ Cardiovasc Genet Volume: 6(6) Pages: 624-33
• [Journal Article] Role of NRSF/REST in the Regulation of Cardiac Gene Expression and Function (2013)
  • Author(s): Kuwahara K
  • Journal Title: Circ J Volume: 77(11) Pages: 2682-6
  • NAID: 10031196813
• [Journal Article] Angiotensin II Type 1a receptor signaling directly contributes to the increased arrhythmogenicity in cardiac hypertrophy (2013)
  • Author(s): Yasuno S, Kuwahara K, Kinoshita H, Yamada C, Nakagawa Y, Usami S, Kuwabara Y, Ueshima K, Harada M, Nishikimi T, Nakao K
  • Journal Title: Br J Pharmacol Volume: 170(7) Pages: 1384-95
• [Journal Article] Adrenomedullin in Cardiovascular Disease : A Useful Biomarker, Its Pathological Roles and Therapeutic Application (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Nakao K
  • Journal Title: Curr Protein Pept Sci Volume: 14(4) Pages: 256-267
• [Journal Article] Increased Expression of HCN Channels in the Ventricular Myocardium Contributes to Enhanced Arrhythmicity in Mouse Failing Hearts (2013)
  • Author(s): Kuwabara Y, Kuwahara K, Takano M, Kinoshita H, Arai Y, Yasuno S, Nakagawa Y, Igata S, Usami S, Minami T, Yamada Y, Nakao K, Yamada C, Shibata J, Nishikimi T, Ueshima K, Nakao K
  • Journal Title: J Am Heart Assoc Volume: 2(3)
• [Journal Article] Cardiac nuclear High Mobility Group Box 1 prevents the development of cardiac hypertrophy and heart failure (2013)
  • Author(s): Funayama A, Shishido T, Netsu S, Narumi T, Kadowaki S, Takahashi H, Miyamoto T, Watanabe T, Woo CH, Abe JI, Kuwahara K, Nakao K, Takeishi Y, Kubota I
  • Journal Title: Cardiovasc Res Volume: 99(4) Pages: 657-64
• [Journal Article] Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure (2013)
  • Author(s): Nishikimi T, Okamoto H, Nakamura M, Ogawa N, Horii K, Nagata K, Nakagawa Y, Kinoshita H, Yamada C, Nakao K, Minami T, Kuwabara Y, Kuwahara K, Masuda I, Kangawa K, Minamino N, Nakao K
  • Journal Title: PLoS One Volume: 8(1)
  • NAID: 120005241437
• [Journal Article] Angiotensin II-induced cardiac hypertrophy and fibrosis are promoted in mice lacking Fgf16 (2013)
  • Author(s): Matsumoto E, Sasaki S, Kinoshita H, Kito T, Ohta H, Konishi M, Kuwahara K, Nakao K, Itoh N
  • Journal Title: Genes Cells Volume: 18(7) Pages: 544-553
• [Journal Article] Voltage-dependent N-type Ca2+ channels in endothelial cells contribute to oxidative stress-related endothelial dysfunction induced by angiotensin II in mice (2013)
  • Author(s): Nishida M, Ishikawa T, Saiki S, Sunggip C, Aritomi S, Harada E, Kuwahara K, Hirano K, Mori Y, Kim-Mitsuyama S
  • Journal Title: Biochem Biophys Res Commun Volume: 434(2) Pages: 210-6
• [Journal Article] Determination of the B-type Natriuretic Peptide Level as a Criterion for Abnormalities in Japanese Individuals in Routine Clinical Practice : The J-ABS Multi-center Study (Japan Abnormal BNP Standard) (2013)
  • Author(s): Kawai M, Yoshimura M, Harada M, Mizuno Y, Hiramitsu S, Shimizu M, Shoda T, Kuwahara K, Miyagishima K, Ueshima K, Nakao K
  • Journal Title: Intern Med Volume: 52(2) Pages: 171-7
  • NAID: 130003365857
• [Journal Article] Role of NRSF/REST in the Regulation of Cardiac Gene Expression and Function. (2013)
  • Author(s): Kuwahara K.
  • Journal Title: Circ J Volume: 77 Pages: 2682-86
  • NAID: 10031196813
  • Peer Reviewed
• [Journal Article] Angiotensin II type 1a receptor signalling directly contributes to the increased arrhythmogenicity in cardiac hypertrophy (2013)
  • Author(s): Yasuno, S., Kuwahara, K., Kinoshita, H., Yamada, C., Nakagawa, Y., Usami, S., Kuwabara, Y., Ueshima, K., Harada, M., Nishikimi, T., Nakao, K.
  • Journal Title: British Journal of Pharmacology Volume: 170(7) Issue: 7 Pages: 1384-1395
  • DOI: 10.1111/bph.12328
  • Peer Reviewed
• [Journal Article] Adrenomedullin in Cardiovascular Disease: A Useful Biomarker, Its Pathological Roles and Therapeutic Application (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Nakao K
  • Journal Title: Curr Protein Pept Sci. Volume: 14 Pages: 256-267
  • Peer Reviewed
• [Journal Article] Increased expression of HCN channels in the ventricular myocardium contributes to enhanced arrhythmicity in mouse failing hearts. (2013)
  • Author(s): Kuwabara, Y., Kuwahara, K., Takano, M., Kinoshita, H., Arai, Y., Yasuno, S., Nakagawa, Y., Igata, S., Usami, S., Minami, T., Yamada, Y., Nakao, K., Yamada, C., Shibata, J., Nishikimi, T., Ueshima, K., Nakao, K.
  • Journal Title: Journal of the American Heart Association Volume: 2(3) Issue: 3
  • DOI: 10.1161/jaha.113.000150
  • Peer Reviewed
• [Journal Article] Cardiac nuclear High Mobility Group Box 1 prevents the development of cardiac hypertrophy and heart failure. (2013)
  • Author(s): Funayama A, Shishido T, Netsu S, Narumi T, Kadowaki S, Takahashi H, Miyamoto T, Watanabe T, Woo CH, Abe JI, Kuwahara K, Nakao K, Takeishi Y, Kubota I.
  • Journal Title: Cardiovasc Res. Volume: 99 Issue: 4 Pages: 657-64
  • DOI: 10.1093/cvr/cvt128
  • Peer Reviewed
• [Journal Article] Voltage-dependent N-type Ca2+ channels in endothelial cells contribute to oxidative stress-related endothelial dysfunction induced by angiotensin II in mice. (2013)
  • Author(s): Nishida M, Ishikawa T, Saiki S, Sunggip C, Aritomi S, Harada E, Kuwahara K, Hirano K, Mori Y, Kim-Mitsuyama S
  • Journal Title: Biochem Biophys Res Commun. Volume: 434 Issue: 2 Pages: 210-216
  • DOI: 10.1016/j.bbrc.2013.03.040
  • Peer Reviewed
• [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure. (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Minamino N, Nakao K.
  • Journal Title: Heart. Volume: 99 Pages: 677-679
  • Peer Reviewed
• [Journal Article] 109th Scientific Meeting of the Japanese Society of Internal Medicine : symposium : 2. Translational research and evidence-based medicine (EBM) in internal medicine in Japan, translational research; 4) Translational research of endocrinology and metabolism (2012)
  • Author(s): Hosoda K, Yasoda A, Ebihara K, Kuwahara K, Mukouyama M, Nakao K
  • Journal Title: Nihon Naika Gakkai Zasshi Volume: 101(9) Pages: 2552-62
• [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure (2012)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Minamino N, Nakao K
  • Journal Title: Heart Volume: 99(10) Pages: 677-679
• [Journal Article] Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodeling in mice (2012)
  • Author(s): Minami T, Kuwahara K, Nakagawa Y, Takaoka M, Kinoshita H, Nakao K, Kuwabara Y, Yamada Y, Yamada C, Shibata J, Usami S, Yasuno S, Nishikimi T, Ueshima K, Sata M, Nakano H, Seno T, Kawahito Y, Sobue K, Kimura A, Nagai R and Nakao K
  • Journal Title: EMBO J Volume: 31(23) Pages: 4428-40
• [Journal Article] In vivo site-specific transfection of naked plasmid DNA in mice using a tissue suction device (2012)
  • Author(s): Shimizu K, Kawakami S, Hayashi K, Kinoshita H, Kuwahara K, Nakao K, Hashida M, Konishi S
  • Journal Title: Plos One Volume: 7(7)
• [Journal Article] Transcriptional regulation of the fetal cardiac gene program (2012)
  • Author(s): Kuwahara K, Nishikimi T, Nakao K
  • Journal Title: J Pharmacol Sci. 18 Volume: 119(3) Pages: 198-203
  • NAID: 10030986728
• [Journal Article] Fixed-dose combination therapy of candesartan cilexetil and amlodipine besilate for the treatment of hypertension in Japan (2012)
  • Author(s): Yasuno S, Fujimoto A, Nakagawa Y, Kuwahara K, Ueshima K
  • Journal Title: Expert Rev Cardiovasc Ther Volume: 10(5) Pages: 577-83
• [Journal Article] The effect of glycosylation on plasma N-terminal proBNP-76 levels in patients with heart or renal failure (2012)
  • Author(s): Nishikimi T, Ikeda M, Takeda Y, Ishimitsu T, Shibasaki I, Fukuda H, Kinoshita H, Nakagawa Y, Kuwahara K, Nakao K
  • Journal Title: Heart Volume: 98(2) Pages: 152-61
• [Journal Article] Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodeling in mice. (2012)
  • Author(s): Minami T, Nakao K, et al
  • Journal Title: EMBO J. Volume: 31 Issue: 23 Pages: 4428-4440
  • DOI: 10.1038/emboj.2012.296
  • Peer Reviewed
• [Journal Article] The effect of glycosylation on plasma N-terminal proBNP-76 levels in patients with heart or renal failure (2012)
  • Author(s): Nishikimi T, Nakao K, et al
  • Journal Title: Heart Volume: 98 Issue: 2 Pages: 152-161
  • DOI: 10.1136/heartjnl-2011-300102
  • Peer Reviewed
• [Journal Article] Fixed-dose combination therapy of candesartan cilexetil and amlodipine besilate for the treatment of hypertension in Japan. (2012)
  • Author(s): Yasuno S, Fujimoto A, Nakagawa Y, Kuwahara K, Ueshima K.
  • Journal Title: Expert Rev Cardiovasc Ther. Volume: 10(5) Issue: 5 Pages: 577-83
  • DOI: 10.1586/erc.12.34
  • Peer Reviewed
• [Journal Article] New Molecular Mechanisms for Cardiovascular Disease : Transcriptional Pathways and Novel Therapeutic Targets in Heart Failure (2011)
  • Author(s): Kuwahara K, Nakao K
  • Journal Title: J Pharmacol Sci. 17 Volume: 116(4) Pages: 337-342
  • NAID: 10029895482
• [Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-a (2011)
  • Author(s): Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, Yamashita JK
  • Journal Title: PLoS One Volume: 6(2)
• [Journal Article] Current biochemistry, molecular biology, and clinical relevance of natriuretic peptides (2011)
  • Author(s): Nishikimi T, Kuwahara K, Nakao K
  • Journal Title: J Cardiol Volume: 57(2) Pages: 131-140
  • NAID: 10031159554
• [Journal Article] Zinc-finger protein 90 negatively regulates neuron-restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes (2011)
  • Author(s): Hata L, Murakami M, Kuwahara K, Nakagawa Y, Kinoshita H, Usami S, Yasuno S, Fujiwara M, Kuwabara Y, Minami T, Yamada Y, Yamada Y, Nakao K, Ueshima K, Nishikimi T, and Nakao K
  • Journal Title: J Mol Cell Cardiol Volume: 50(6) Pages: 972-981
• [Journal Article] New Molecular Mechanisms for Cardiovascular Disease:Transcriptional Pathways and Novel Therapeutic Targets in Heart Failure (2011)
  • Author(s): Kuwahara K, Nakao K
  • Journal Title: Journal of Pharmacological Sciences Volume: 116 Issue: 4 Pages: 337-342
  • DOI: 10.1254/jphs.10R28FM
  • NAID: 10029895482
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-a (2011)
  • Author(s): Fujiwara M, Kuwahara K, Yamanaka S, Nakao K, Yamashita
  • Journal Title: PLoS One Volume: 6(2) Issue: 2 Pages: e16734-e16734
  • DOI: 10.1371/journal.pone.0016734
  • Peer Reviewed
• [Presentation] TRPC3/6 as Potentially Novel Therapeutic Targets for the Treatment of Pulmonary Arterial Hypertension (2014)
  • Author(s): Kinoshita H, Kuwahara K
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京
• [Presentation] Elucidation of molecular mechanism of human proBNP : role of O-glycosylation in N-terminal region of proBNP (2014)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Yamada C, Ueshima K, Minamino N, Kimura T, Nakao K
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京
• [Presentation] Molecular mechanism of lower processing rate in human proBNP : importance of the distance between the O-glycosylation and cleavage sites (2014)
  • Author(s): Nishikimi T, Nakagawa Y, Minamino N, Ikeda M, Ishimitsu T, Yamada C, Nakao K, Minami T, Kuwabara Y, Kinoshita H, Kangawa K, Kuwahara K, Nakao K
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京
• [Presentation] ナトリウム利尿ペプチド発現制御機構解明による心血管病治療標的の同定 (2013)
  • Author(s): 桑原宏一郎
  • Organizer: 第17回日本心血管内分泌代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] 維持血液透析患者における血中B型(脳性)ナトリウム利尿ペプチド前駆体についての検討 (2013)
  • Author(s): 中川靖章, 錦見俊雄, 桑原宏一郎, 保野慎治, 木下秀之, 桒原佳宏, 中尾一泰, 山田千夏, 上嶋健治, 中尾一和
  • Organizer: 第17回日本心血管内分泌代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] 維持血液透析患者におけるproBNPについての検討 (2013)
  • Author(s): 中川靖章, 錦見俊雄, 桑原宏一郎, 保野慎治, 木下秀之, 桒原佳宏, 中尾一泰, 山田千夏, 上嶋健治, 中尾一和
  • Organizer: 第17回日本心不全学会学術集会
  • Place of Presentation: 埼玉
• [Presentation] 透析患者における血中N端proBNPの糖鎖修飾と測定系への影響について (2013)
  • Author(s): 中川靖章, 錦見俊雄, 桑原宏一郎, 保野慎治, 木下秀之, 桒原佳宏, 中尾一泰, 山田千夏, 上嶋健治, 中尾一和
  • Organizer: 第2回臨床高血圧フォーラム
  • Place of Presentation: 東京
• [Presentation] 肺動脈性肺高血圧症に対する新規治療標的としてのTRPC3/6阻害効果の検討 (2013)
  • Author(s): 木下秀之, 桑原宏一郎, 清中茂樹, 森泰生, 赤木達, 中村一文, 桒原佳宏, 中川靖章, 錦見俊雄, 中尾一和
  • Organizer: 第2回日本肺循環学会学術集会
  • Place of Presentation: 東京
• [Presentation] Myocardin-related transcription factor-A plays a pivotal role in vascular remodeling (2013)
  • Author(s): Kuwahara K, Nakao K
  • Organizer: 第45回日本動脈硬化学会
  • Place of Presentation: 東京
• [Presentation] 透析患者における血中B型(脳性)ナトリウム利尿ペプチド前駆体proBNPについての検討 (2013)
  • Author(s): 中川靖章, 錦見俊雄, 桑原宏一郎, 保野慎治, 木下秀之, 桒原佳宏, 中尾一泰, 山田千夏, 上嶋健治, 中尾一和
  • Organizer: 第61回心臓病学会
  • Place of Presentation: 熊本
• [Presentation] Glycosylation of plasma N-terminal proBNP and its effect on NT-proBNP assay in hemodyalysis patients (2013)
  • Author(s): Yasuaki Nakagawa, Toshio Nishikimi, , Koichiro Kuwahara Yoshihiro Kuwabara, , Hideyuki Kinoshita, Makoto Takano, Satoru Usami, Kazuhiro Nakao, Takeya Minami, Yuko Yamada, Chinatsu Yamada, Junko Shibata, Kenji Ueshima and Kazuwa Nakao
  • Organizer: 第77回日本循環器学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Effect of hemodyalysis on precursor proBNP and glycosylated NT-proBNP in end-stage renal disease patients (2013)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Yamada C, Nakao K, Ueshima K, Nakao K
  • Organizer: European Society of Cardiology 2013 (ESC 2013)
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] Blockade of TRPC6 is a Novel Therapeutic Approach Against Pathological Cardiac Remodeling (2013)
  • Author(s): Kinoshita H, Kuwahara K, Nishida M, Kurose H, Kiyonaka S, Mori Y, Yamada C, Kuwabara Y, Yasuno S, Nakagawa Y, Nishikimi T, Ueshima K, Nakao K
  • Organizer: International Conference on Endothelin ET-13
  • Place of Presentation: Tokyo
• [Presentation] O-glycosylated Pro B-type Natriuretic Peptide is Cleaved intracellularly By Furin in Ventricular Myocytes and Atrial Myocytes : Importance of the Distance Between the O-glycosylation Sites and Cleaved Sites (2013)
  • Author(s): Nishikimi T, Nakagawa Y, Minamino N, Ikeda M, Ishimitsu T, Yamada C, Nakao K, Minami T, Kuwabara Y, Kinoshita H, Kangawa K, Kuwahara K, Nakao K
  • Organizer: AHA Scientific Sessoins 2013
  • Place of Presentation: Dallas, USA
• [Presentation] 血管壁ナトリウム利尿ペプチド系の意義の解明－血管内皮細胞特異的C型ナトリウム利尿ペプチド(CNP)ノックアウトマウスの解析－ (2013)
  • Author(s): 中尾一泰 桑原宏一郎 錦見俊雄 中川靖章 木下秀之 宇佐美覚 桑原佳宏 南丈也 山田千夏 山田優子 保野慎治 上嶋健治 曽根正勝 寒川賢治 中尾一和
  • Organizer: 第50回 臨床分子医学会
  • Place of Presentation: 東京
• [Presentation] 拡張型心筋症モデルマウスにおける左室リモデリング及び不整脈源性基質形成におけるレニン―アンジオテンシン系の意義 (2013)
  • Author(s): 山田 千夏, 桑原 宏一郎,木下 秀之, 錦見 俊雄, 中尾 一泰, 桒原 佳宏,中川 靖章, 中尾 一和
  • Organizer: 第50回 臨床分子医学会
  • Place of Presentation: 東京
• [Presentation] 左室リモデリング進行及び催不整脈性抑制におけるレニンーアンジオテンシン系の検討 (2013)
  • Author(s): 山田千夏、桑原宏一郎、木下秀之、錦見俊雄、中尾一泰、桒原佳宏、保野慎治、中川靖章、上嶋健治、中尾一和
  • Organizer: 第86回 日本内分泌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] 肺動脈性肺高血圧症に対する新規治療標的としてのTRPC3/6阻害効果の検討 (2013)
  • Author(s): 木下秀之、桑原宏一郎、清中茂樹、森泰生、赤木達、中村一文、桒原佳宏、中川靖章、錦見俊雄、中尾一和
  • Organizer: 第2回 日本肺循環学会学術集会
  • Place of Presentation: 東京
• [Presentation] Contribution of renin-angiotensin system to the generation of arrhythmogenic substrate in mice with dilated cardiomyopathy and lethal arrhythmia (2013)
  • Author(s): Chinatsu Yamada, Koichiro Kuwahara
  • Organizer: 第30回 国際心臓研究学会(ISHR)日本部会
  • Place of Presentation: Ｓａｎ Ｄｉｅｇｏ
• [Presentation] Elucidation of molecular mechanisms underlying the regulation and action of cardiovascular hormones to find novel therapeutic targets for cardiovascular diseases (2013)
  • Author(s): Koichiro Kuwahara
  • Organizer: The77th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Multi-organ communication in sudden arrhythmic death accompanied with heart failure (2013)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao
  • Organizer: The77th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 横浜
• [Presentation] Translational Research of Natriuretic Peptide in Cardiovascular Disease (2012)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: 福岡国際会議場・福岡(招待講演)
  • Year and Date: 2012-03-18
• [Presentation] Increased HCN channels in failing hearts possibly contribute to ventricular arrhythmias (2012)
  • Author(s): Yoshihiro Kuwabara, Koichiro Kuwahara, 他
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: 福岡国際会議場・福岡
  • Year and Date: 2012-03-17
• [Presentation] MRTF-A, a rho-dependent co-activator of SRF, plays a critical role in vascular remodeling by regulating smooth muscle cell migration (2012)
  • Author(s): Takeya Minami, Koichiro Kuwahara, 他
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: 福岡国際会議場・福岡
  • Year and Date: 2012-03-17
• [Presentation] TRPC3/6 Ion Channels as Potentially Novel Therapeutic Targets against Pulmonary Arterial Hypertension (2012)
  • Author(s): Hideyuki Kinoshita, Koichiro Kuwahara, 他
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: 福岡国際会議場・福岡
  • Year and Date: 2012-03-17
• [Presentation] Translational Research of Natriuretic Peptide in Cardiovascular Disease (2012)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao (Meet the Experts)
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: Fukuoka International Congress Center, Fukuoka, Japan
• [Presentation] TRPC3/6 Ion Channels as Potentially Novel Therapeutic Targets against Pulmonary Arterial Hypertension (2012)
  • Author(s): Hideyuki Kinoshita, Koichiro Kuwahara, Shigeki Kiyonaka, Yasuo Mori, Junko Shibata, Chinatu Yamada, Takeya Minami, Kazuhiro Nakao, Yoshihiro Kuwabara, Shinji Yasuno, Satoru Usami, Yasuaki Nakagawa, Toshio Nishikimi, Kenji Ueshima, KazuwaNakao
  • Organizer: The76th Annual Scientific Meeting of The Japanese CirculationSociety
  • Place of Presentation: Fukuoka International Congress Center, Fukuoka, Japan
• [Presentation] Molecular mechanisms underlying cardioprotective effects of natriuretic peptide (2012)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao
  • Organizer: The 7th Oulu Symposium
  • Place of Presentation: Oulu, Finland
• [Presentation] 血管リモデリングにおける新規治療標的としてのRho依存性転写共役因子MRTF-Aの意義 (2012)
  • Author(s): 南 丈也・桑原宏一郎・中川靖章・木下秀之・中尾 一泰・桒原佳宏・錦見俊雄・中尾一和
  • Organizer: 第49回日本臨床分子医学会
  • Place of Presentation: 京都
• [Presentation] ｍｉＲ－１を介したＲｈｏ依存性転写共役因子ＭＲＴＦ－Ａ発現制御の血管リモデリングにおける意義 (2012)
  • Author(s): 南 丈也・桑原宏一郎・中川靖章・木下秀之・中尾 一泰・桒原佳宏・錦見俊雄・中尾一和
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 拡張型心筋症マウスにおけるN型Ca2+チャネル阻害による自律神経活性調節を介した不整脈突然死 予防の有用性 (2012)
  • Author(s): 山田優子、桑原宏一郎、木下秀之、桑原佳宏、中 川靖章、柴田純子、山田千夏、南丈也、保野慎 治、宇佐美覚、中尾一泰、錦見俊雄、上嶋健治、 森泰生、中尾一和
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 慢性心不全・突然死モデルマウスの不整脈源性基質形成における、レニンーアンジオテンシン系の関 与の検討 (2012)
  • Author(s): 山田千夏 桑原宏一郎 木下秀之 桒原佳宏 南 丈也 中尾一泰 宇佐美覚 保野慎治 中川靖章 錦見俊雄 上嶋健治 中尾一和
  • Organizer: 第29回日本心電学会学術集会
  • Place of Presentation: 幕張
• [Presentation] N-Type Ca2+ Channel Blockade Prevents Sudden Death in Mice with Heart Failure (2012)
  • Author(s): Yuko Yamada, Koichiro Kuwahara, Hideyuki Kinoshita, Yoshihiro Kuwabara, Yasuaki Nakagawa, Chinatsu Yamada, Takeya Minami, Shinji Yasuno, Kazuhiro Nakao, Kazuwa Nakao, et al.
  • Organizer: 第29回国際心臓研究学会(ISHR)日本部会
  • Place of Presentation: 福岡
• [Presentation] Reciprocal expression of MRTF-A and Myocardin is crucial for pathological vascular remodeling in mice (2012)
  • Author(s): Takeya Minami, Koichiro Kuwahara, Yasuaki Nakagawa, Hideyuki Kinoshita, Kazuhiro Nakao, Yoshihiro Kuwabara, Toshio Nishikimi, Kazuwa Nakao
  • Organizer: 第29回国際心臓研究学会(ISHR)日本部会
  • Place of Presentation: 福岡
• [Presentation] Neurohumoral regulation in the process of pathological cardiac remodeling (2012)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao
  • Organizer: 第29回国際心臓研究学会(ISHR)日本部会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] 左室リモデリング進行及び催不整脈性抑制におけるレニン阻害の有用性の検討 (2012)
  • Author(s): 山田 千夏 桑原 宏一郎 木下 秀之 錦見 俊雄 桑原 佳宏 南 丈也 中尾 一泰 中川 靖章 中 尾 一和
  • Organizer: 第15回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 東京
• [Presentation] Reciprocal expression of MRTF-A and myocardin mediated by miR-1 is crucial for phenotypic modulation of vascular smooth muscle cells (2012)
  • Author(s): Takeya Minami, Koichiro Kuwahara, Yasuaki Nakagawa, Hideyuki Kinoshita, Kazuhiro Nakao, Yoshihiro Kuwabara, Yuko Yamada, Chinatsu Yamada, Junko Shibata, Kazuwa Nakao, et al.
  • Organizer: Arteriosclerosis, Thrombosis and Vascular BiologyScientific Session 2012
  • Place of Presentation: Chicago
• [Presentation] Molecular mechanisms underlying cardioprotective effects of natriuretic peptides (2012)
  • Author(s): Koichiro Kuwahara, Kazuwa Nakao
  • Organizer: The 7th Oulu Symposium
  • Place of Presentation: Oulu
  • Invited
• [Presentation] Overexpression of HCN Channels in Failing Heart Contributes to Increased Ventricular Arrhythmicity (2012)
  • Author(s): Yoshihiro Kuwabara, Koichiro Kuwahara, Hideyuki Kinoshita, Makoto Takano, Yasuaki Nakagawa, Satoru Usami, Kazuhiro Nakao, Takeya Minami, Yuko Yamada, Chinatsu Yamada, Kazuwa Nakao, et al.
  • Organizer: Basic Cardiovascular Sciences 2012 Scientific Sessions
  • Place of Presentation: New orleans
• [Presentation] Direct renin inhibitor prevents ventricular remodeling and sudden arrhythmic death in mice with dilated cardiomyopathy (2012)
  • Author(s): C. Yamada, K. Kuwahara, T. Minami, Y. Kuwabara, K. Nakao, H. Kinoshita, S. Usami, Y. Nakagawa, T. Nishikimi, K. Nakao
  • Organizer: 2012年欧州心臓学会議
  • Place of Presentation: Munich
• [Presentation] Direct Renin Inhibitor Strongly Prevents Ventricular Arrhythmogenic Remodeling and Sudden Cardiac Death in Mice with Dilated Cardiomyopathy (2012)
  • Author(s): Chinatsu Yamada, Koichiro Kuwahara, Hideyuki Kinoshita, Yoshihiro Kuwabara, Takeya Minami, Kazuhiro Nakao, Shinji Yasuno, Yasuaki Nakagawa, Kenji Ueshima, Kazuwa Nakao,
  • Organizer: 2012年米国心臓病学会議
  • Place of Presentation: Los Angelえs
• [Presentation] ANP・BNP遺伝子発現調節因子NRSFにより制御されるHCNチャネルの心不全モデルマウスにおける突然死発症への関与 (2011)
  • Author(s): 桑原佳宏, 桑原宏一郎, 他
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター・大阪
  • Year and Date: 2011-11-26
• [Presentation] 病的血管リモデリングでみられる血管平滑筋細胞形質転換におけるRho依存性転写共役因子MRTF-Aの意義 (2011)
  • Author(s): 南丈也、桑原宏一郎, 他
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター・大阪
  • Year and Date: 2011-11-26
• [Presentation] BNP遺伝子発現調節領域におけるserum response factor応答領域の新たなる同定とその心筋細胞肥大反応における意義 (2011)
  • Author(s): 桑原宏一郎, 他
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター・大阪
  • Year and Date: 2011-11-26
• [Presentation] Inhibition of HCN overexpression in failing heart of dilated cardiomyopathy mouse model by ivabradine prevents sudden arrhythmic (2011)
  • Author(s): Yoshihiro Kuwabara, Koichiro Kuwahara, 他
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-11-15
• [Presentation] N-Type Ca2+ Channel Blocker is Effective to Prevent Sudden Cardiac Death in Mice with Heart Failure (2011)
  • Author(s): Yuko Yamada, Koichiro Kuwahara, 他
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-11-14
• [Presentation] Induction of Myocardin-Related Transcription Factor-A Contributes to the Phenotypic Alteration of Vascular Smooth Muscle Cells and Plays a (2011)
  • Author(s): Takeya Minami, Koichiro Kuwahara, 他
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Orlando, USA
  • Year and Date: 2011-11-14
• [Presentation] 拡張型心筋症・不整脈死モデルマウスにおけるN型Ca2+チャネル阻害の有用性の検討 (2011)
  • Author(s): 山田優子、桑原宏一郎, 他
  • Organizer: 第34回日本日本高血圧学会総会
  • Place of Presentation: 栃木県総合文化センター・宇都宮
  • Year and Date: 2011-10-22
• [Presentation] Rho依存性SRF共役転写因子MRTF-Aは血管リモデリングを制御する (2011)
  • Author(s): 南丈也・桑原宏一郎, 他
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場・神戸
  • Year and Date: 2011-04-23
• [Presentation] 受容体活性化型Ca2+チャネルTRPC3/6阻害の病的心肥大抑制効果 (2011)
  • Author(s): 木下秀之、桑原宏一郎, 他
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場・神戸
  • Year and Date: 2011-04-23
• [Presentation] ANP・BNP遺伝子発現調節因子NRSFの機能阻害による心筋症モデルマウスにおける不整脈発症・突然死に対するHCNチャネル阻害の効果 (2011)
  • Author(s): 桑原佳宏, 桑原宏一郎, 他
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場・神戸
  • Year and Date: 2011-04-23
• [Presentation] 心房性および脳性ナトリウム利尿ペプチドの発現制御及び心不全発症における役割 (2011)
  • Author(s): 桑原宏一郎、中尾一和
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場・神戸(招待講演)
  • Year and Date: 2011-04-23
• [Presentation] バセドウ病を合併した滲出性収縮性心膜炎の一例 (2011)
  • Author(s): 山田千夏、桑原宏一郎, 他
  • Organizer: 第22回日本心エコー学会学術集会
  • Place of Presentation: 鹿児島市民文化ホール・鹿児島
  • Year and Date: 2011-04-21
• [Presentation] BNP遺伝子発現調節領域におけるserum response factor応答領域の新たなる同定とその心筋細胞肥大反応における意義 (2011)
  • Author(s): 桑原宏一郎・ 木下秀之・ 桒原佳宏・ 中川靖章・ 南 丈也・ 山田千夏・ 柴田純子・宇佐美覚・ 錦見俊雄・ 中尾一和
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 大阪
• [Presentation] Blockade of TRPC6/3 is a Novel Therapeutic Approach for Preventing Pathological Cardiac Hypertrophy (2011)
  • Author(s): Hideyuki Kinoshita, Koichiro Kuwahara, Motohiro Nishida, Hitoshi Kurose, Shigeki Kiyonaka, Yasuo Mori, Yoshihiro Kuwabara, Yasuaki Nakagawa, Kenji Ueshima, Kazuwa Nakao
  • Organizer: European Society of Cardiology Congress
  • Place of Presentation: Paris, France
• [Presentation] type Ca2+ channel blockade prevents sudden death in mice with heart failu (2011)
  • Author(s): Yuko Yamada, Koichiro Kuwahara, 他
  • Organizer: Basic Cardiovascular Sciences 2011 Scientific Sessions
  • Place of Presentation: New Orleans, USA
• [Presentation] MRTF-A, a Rho-dependent coactivator of SRF, plays a critical role in vascular remodeling (2011)
  • Author(s): Takeya Minami, Koichiro Kuwahara, 他
  • Organizer: Basic Cardiovascular Sciences 2011 Scientific Sessions
  • Place of Presentation: New Orleans, USA
• [Presentation] HCN channel inhibition with ivabradine prevents sudden arrhythmic death in a mouse model of dilated cardiomyopathy (2011)
  • Author(s): Yoshihiro Kuwabara, Koichiro Kuwahara, 他
  • Organizer: European Society of Cardiology Congress2011
  • Place of Presentation: Paris, France
• [Presentation] Blockade of TRPC6/3 is a Novel Therapeutic Approach for Preventing Pathological Cardiac Hypertrophy (2011)
  • Author(s): Hideyuki Kinoshita, Koichiro Kuwahara, 他
  • Organizer: European Society of Cardiology Congress2011
  • Place of Presentation: Paris, France
• [Presentation] N-type Ca2+ channel blockade prevents sudden arrhythmic death in ice with heart failure (2011)
  • Author(s): Yuko Yamada, Koichiro Kuwahara, 他
  • Organizer: 4th Asia Pacific Heart Rhythm Society Scientific Session (APHRS2011)
  • Place of Presentation: ヒルトン福岡シーホーク・福岡
• [Presentation] Inhibition of HCN overexpressed in failing heart of dilated cardiomyopathy mouse model by ivabradine prevents sudden arrhythmic (2011)
  • Author(s): Yoshihiro Kuwabara, Koichiro Kuwahara, 他
  • Organizer: 4th Asia Pacific Heart Rhythm Society Scientific Session (APHRS2011)
  • Place of Presentation: ヒルトン福岡シーホーク・福岡
• [Book] ガイドライン外来診療2014 (2014)
  • Author(s): 桑原宏一郎, 木村剛
  • Publisher: 日経メディカル開発
• [Book] ガイドライン外来診療2014 (2014)
  • Author(s): 桑原宏一郎、木村剛
  • Total Pages: 678
  • Publisher: 日経メディカル開発
• [Book] 変貌する心不全診療 (2013)
  • Author(s): 中川靖章, 桑原宏一郎
  • Publisher: 南江堂
• [Book] 最新内分泌代謝学 (2013)
  • Author(s): 桑原宏一郎
  • Publisher: 診断と治療社
• [Book] 変貌する心不全治療 (2013)
  • Author(s): 中川靖章、桑原宏一郎など
  • Total Pages: 310
  • Publisher: 南江堂
• [Book] 心不全診療Q&Aエキスパート106人からの回答 (2012)
  • Author(s): 桑原宏一郎
  • Publisher: 中外医学社
• [Book] 心不全診療Q&A-エキスパート64人からの回答 (2012)
  • Author(s): 桑原宏一郎など
  • Total Pages: 446
  • Publisher: 中外医学社
• [Book] 新腎臓内科学 (2011)
  • Author(s): 中尾一和監修
  • Total Pages: 207
  • Publisher: 診断と治療社
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/cardio.html
• [Remarks] 京都大学大学院医学研究科内科学講座 内分泌代謝内科学 心臓グループ
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/cardio.html
• [Remarks] 京都大学大学院医学研究科内科学講座 内分泌代謝内科学 心臓グループ
  • URL: http://www.kuhp.kyoto-u.ac.jp/̃med2/jpn/research/cardio.html
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/index-jp.html