Development of a new rapid immunohistochemical staining method using AC electric field
| Project/Area Number |
23390311
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKAGAMI Yoichi , 秋田県産業技術センター, 副センター長 (00373217)
谷野 美智枝 北海道大学, 医学(系)研究科(研究院), 講師 (90360908)
小川 純一 秋田大学, 医学(系)研究科(研究院), 教授 (20112774)
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| Co-Investigator(Renkei-kenkyūsha) |
TANINO Mishie 北海道大学, 大学院医学系研究科, 講師 (90360908)
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| Research Collaborator |
NANJO Hiroshi
NAKAMURA Ryuta
TANAKA Shinya
ITO Tomoo
KAGAYA Masami
KONNO Hayato
SASAJIMA Toshio
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 免疫組織染色 / 癌 / 手術 / 術中迅速診断 / リンパ節微小転移 / 肺癌 / 脳腫瘍 / 病理 / 免疫祖織染色 / リンパ節転移 / 電界撹拌技術 |
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| Outline of Final Research Achievements |
For the intraoperative diagnosis of lymph-node micro-metastases with immunohistochemical staining, the staining time should be shortened. We invented a new method that allows to reduce the immunohistochemical-staining time by applying an electric field to the tissue sections. We can complete immunohistochemical staining which usually requires 2 hours, within 12 minutes using this method. We demonstrated that this method was useful for the intraoperative detection of lymph node micrometastases of patients with lung cancer. We also demonstrated that this method is useful for the grading of the glioma during surgery. We tried this method to the decision making of the surgery for lung cancer and brain tumor. We also tried this method to the decision making of the surgery of gastroenterological, gynecologic, pediatric cancer and cancer of otorhinolaryngology. And then, we were able to demonstrate the usefulness of this method for the decision making of these surgeries
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors.2015
Author(s)
Tanino M, Sasajima T, Nanjo H, Akesaka S, Kagaya M, Kimura T, Ishida Y, Oda M, Takahashi M, Sugawara T, Yoshioka T, Nishihara H, Akagami Y, Goto A, Minamiya Y, Tanaka S; R-IHC Study Group.
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Journal Title
Brain Tumor Pathol.
Volume: 32
Issue: 1
Pages: 12-19
DOI
NAID
Related Report
Peer Reviewed / Open Access
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