Verification study of sleep bruxism pathogenesis focused on the serotonin nerve activities as neuromodulator

• Project/Area Number: 23390442
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Okayama University
• Principal Investigator: MINAKUCHI Hajime 岡山大学, 大学病院, 講師 (30325097)
• Co-Investigator(Kenkyū-buntansha): KUBOKI Takuo 岡山大学, 大学院医歯薬学総合研究科, 教授 (00225195) ; SOGAWA Chiharu 岡山大学, 大学院医歯薬学総合研究科, 助教 (10253022) ; MAEKAWA Kenji 岡山大学, 大学院医歯薬学総合研究科, 准教授 (20304313) ; SOGAWA Norio 松本歯科大学, 歯学部, 教授 (30236153) ; KITAYAMA Shigeo 岡山大学, 大学院医歯薬学総合研究科, 教授 (80177873) ; 松香 芳三 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (90243477)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 睡眠時ブラキシズム / セロトニン神経系 / トランスポーター / 筋電図 / 睡眠障害 / 口腔運動器疾患 / 5-HT神経系 / セロトニントランスポーター / 日間変動 / 簡易貼付型測定装置 / 簡易貼付型睡眠時ブラキシズム測定装置 / PSG検査 / 睡眠学 / 顎関節症 / 臨床疫学 / モノアミン神経系 / 歯科疾患 / 臨床疫学研究

Outline of Final Research Achievements

This aimed to evaluate the correlation between SERT uptake ability in human peripheral platelets and SB frequency. Subjects were consecutively recruited from sixth-year students. SB frequency was determined as the summary score of 3-consecutive night assessments using a self-contained EMG detector/analyzer in their home environment. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. Amount of SERTs and platelets were quantified by ELISA assay. Functional characterization of SERT, 5-HT uptake, maximum velocity (Vmax) and affinity constant (Km) were assessed by [3H] 5-HT uptake assay. The correlation between these aforementioned variables and SB level was evaluated.
A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (p<0.05, R2=0.063, Spearman correlation). Platelet serotonin uptake is significantly correlated to SB frequency, although it only explains a small amount of SB variability.

Research Products

• [Journal Article] Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control (2014)
  • Author(s): Hajime Minakuchi, Chiharu Sogawa C, Emilio Satoshi Hara, Haruna Miki, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn T Clark, Takuo Kuboki
  • Journal Title: Journal of Prosthodontic Research Volume: 58 Issue: 4 Pages: 217-222
  • DOI: 10.1016/j.jpor.2014.06.003
  • NAID: 130004714111
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multiple sleep bruxism data collected using a self-contained EMG detector/analyzer system in asymptomatic healthy subjects. (2012)
  • Author(s): Minakuchi H, Sakaguchi C, Hara ES, Maekawa K, Matsuka Y, Clark GT, Kuboki T.
  • Journal Title: Sleep Breath. Volume: 16 Issue: 4 Pages: 1069-1072
  • DOI: 10.1007/s11325-011-0602-1
  • Peer Reviewed
• [Journal Article] Occlusal Dysesthesia: a qualitative systematic review of the epidemiology, aetiology and management (2012)
  • Author(s): Hara ES et al
  • Journal Title: Journal of Oral Rehabilitation Volume: 39 Issue: 8 Pages: 630-638
  • DOI: 10.1111/j.1365-2842.2012.02300.x
  • Peer Reviewed
• [Journal Article] Multiple sleep bruxism data collected using a self-contained EMG det ec tor/analyzer system in asymptomatic healthy subjects (2012)
  • Author(s): Minakuchi H, Sakaguchi C, Hara ES, Maekawa K, Matsuka Y, Clark GT, Kuboki T
  • Journal Title: Sleep and Breathing Volume: 26(in press)
  • Peer Reviewed
• [Journal Article] Test-retest reliability of MRI-based disk position diagnosis of the temporomandibular joint (2012)
  • Author(s): Nagamatsu-Sakaguchi C, Maekawa K, Ono T, Yanagi Y, Minakuchi H, Miyawaki S, Asaumi J
  • Journal Title: Clinical Oral Investigatoins Volume: 16(1) Pages: 101-108
  • Peer Reviewed
• [Presentation] Validation Study of a Modified Miniature Bruxism Detection/analyzing Device (2015)
  • Author(s): Miki H, Minakuchi H, Ueda M, Shigemoto S, Suzuki Y, Maekawa K, Matsuka Y, Kuboki T.
  • Organizer: 93th International Association for Dental Research (IADR)
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-12 – 2015-03-14
• [Presentation] The miniature all-in-one bruxism detection/analyzing device -Overview and clinical/research application- (2014)
  • Author(s): Hajime Minakuchi
  • Organizer: 42nd Annual conference of Indian Prosthodontic Sciety
  • Place of Presentation: Chandigarh, India
  • Year and Date: 2014-11-06 – 2014-11-09
  • Invited
• [Presentation] 簡易貼付型睡眠時ブラキシズム測定装置の測定精度の検討 (2014)
  • Author(s): 三木春奈，水口 一，上枝麻友，重本修伺，鈴木善貴，前川賢治，松香芳三，窪木拓男
  • Organizer: 平成26年度日本補綴歯科学会中国・四国，関西支部合同学術大会
  • Place of Presentation: 倉敷市，日本
  • Year and Date: 2014-09-06 – 2014-09-07
• [Presentation] 簡易貼付型睡眠時ブラキシズム測定装置の測定精度の検討 (2014)
  • Author(s): 三木春奈，水口 一，上枝麻友，重本修伺，鈴木善貴，前川賢治，松香芳三，窪木拓男
  • Organizer: 第27回日本顎関節学会学術大会
  • Place of Presentation: 博多，日本
  • Year and Date: 2014-07-09 – 2014-07-10
• [Presentation] 簡易貼付型睡眠時ブラキシズム測定装置の臨床試用について (2014)
  • Author(s): 水口 一
  • Organizer: 27回日本顎関節学会学術大会
  • Place of Presentation: 博多，日本
  • Year and Date: 2014-07-09
  • Invited
• [Presentation] 簡易貼付型睡眠時ブラキシズム測定装置の測定精度の検討 (2014)
  • Author(s): 三木春奈，水口 一，上枝麻友，重本修伺，鈴木善貴，前川賢治，松香芳三，窪木拓男
  • Organizer: 第27回日本顎関節学会総会・学術大会
  • Place of Presentation: 博多
• [Presentation] 簡易貼付型睡眠時ブラキシズム測定装置の臨床試用について (2014)
  • Author(s): 水口 一
  • Organizer: 第27回日本顎関節学会総会・学術大会
  • Place of Presentation: 博多
  • Invited
• [Presentation] Sleep bruxism frequency and 5-HT uptake ability of human peripheral serotonin transporter (2012)
  • Author(s): Minakuchi H, Miki H, Sogawa C*, Hara ES, Fujisawa T, Maekawa K, Matsuka Y, Sogawa N, Kitayama S, Kuboki T
  • Organizer: IADR/LAR General Session & Exhibition
  • Place of Presentation: Iguacu, Brazil
• [Presentation] 睡眠時ブラキシズム頻度と末梢セロトニントランスポーター輸送能との関連 (2012)
  • Author(s): 水口 一，三木春奈，十川千春，Hara Emilio Satoshi，藤澤拓生，前川賢治，松香芳三，十川紀夫，北山滋雄，窪木拓男
  • Organizer: 日本補綴歯科学会 第121回学術大会
  • Place of Presentation: 横浜，日本
• [Presentation] Sleep bruxism frequency and 5-HT uptake ability of human peripheral serotonin transporter (2011)
  • Author(s): Minakuchi H, Sogawa C, Hara ES, Miki H, Fujisawa T, Maekawa K, Matsuka Y, Sogawa N, Kitayama S, Kuboki T
  • Organizer: The 7th Biennial Meeting of Asian Academy of Prosthodntics
  • Place of Presentation: Shanghai, China
  • Year and Date: 2011-10-30
• [Presentation] 睡眠時ブラキシズム頻度と末梢セロトニントランスポーターの機能との関連 (2011)
  • Author(s): 三木春奈, 水口一, Emilio S.Hara, 十川千春, 大野充昭, 藤澤拓生, 前川賢治, 十川紀夫, 松香芳三, 北山滋雄, 窪木拓男
  • Organizer: 第16回日本口腔顔面痛学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-10-09
• [Presentation] 睡眠時ブラキシズム発症頻度と末梢セロトニントランスポーター機能 (2011)
  • Author(s): 水口一, Emilio S.Hara, 三木春奈, 藤澤拓生, 前川賢治, 松香芳三, 窪木拓男
  • Organizer: 第23回日本顎関節学会
  • Place of Presentation: 広島
  • Year and Date: 2011-07-23
• [Presentation] Relationship between sleep bruxism frequency and uptake ability of human peripheral serotonin transporter (2011)
  • Author(s): Minakuchi H, Hara ES, Sogawa C, Miki H, Fujisawa T, Maekawa K, Matsuka Y, Sogawa N, Kitayama S, Kuboki T
  • Organizer: 25th Anniversary Meeting of the Associated Professional Sleep Societies, A joint meeting of the American Academy of Sleep Medicine and the Sleep Research Society
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2011-06-14
• [Presentation] 口腔インプラント手術後疼痛ならびに腫脹感の経時的変化と関連因子の検討-携帯電話端末を情報入力源としたEcological Momentary Assessmentの応用- (2011)
  • Author(s): 黒井隆太, 水口一, 藤原彩, 松香芳三, 岡田宏基, 窪木拓男
  • Organizer: 日本補綴歯科学会第120回学術大会
  • Place of Presentation: 広島
  • Year and Date: 2011-05-21
• [Book] 別冊the Quintessence TMD YEAR BOOK 2011アゴの痛みに対処する (2012)
  • Author(s): 水口一
  • Publisher: クインテッセンス出版
• [Book] 日本歯科評論 (2012)
  • Author(s): 水口一, 窪木拓男
  • Publisher: ヒョーロン・パブリッシャーズ
• [Remarks]
  • URL: http://www.dent.okayama-u.ac.jp/1hotetu/