Generation of transplantable and gene therapy model rats using IL2 receptor gamma KO rat derived from rat pluripotent stem cell
Project/Area Number |
23500490
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | The University of Tokyo |
Principal Investigator |
HAMANAKA SANAE 東京大学, 医科学研究所, 特任研究員 (40511415)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | X-SCID / 移植 / ラットES細胞 / ラットiPS細胞 / IL2レセプターγ遺伝子 |
Research Abstract |
We generated a model rat of the X-linked serious case combined immunodeficiency disease (X-SCID) by homologous recombination with IL2 receptor gammma gene using rat pluripotent stem cells. T cells, B cells and natural killer cells and the thymus were decreased in the X-SCID rat and it showed the serious case immune deficiency disorder. It was suggested that the X-SCID rat was useful as a model of the cell transplantation of a neoplastic cell of human and mouse hematopoietic stem cells, even though the human hematopoietic stem cells were not able to reconstitution.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method2013
Author(s)
Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H
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Journal Title
Biochem Biophys Res Commun
Volume: 435(4)
Pages: 586-91
Related Report
Peer Reviewed
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[Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method2013
Author(s)
Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H
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Journal Title
Biochem Biophys Res Commun
Volume: i435(4)
Issue: 4
Pages: 586-91
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming2012
Author(s)
Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H
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Peer Reviewed
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[Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.2012
Author(s)
Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
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Peer Reviewed
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[Journal Article] Generation of germline-competent rat induced pluripotent stem cells2011
Author(s)
Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H
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Peer Reviewed
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[Journal Article] Generation of germline-competent rat induced pluripotent stem cells.2011
Author(s)
Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H.
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Related Report
Peer Reviewed
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