Development of disease model pigs suitable for diabetic complications research
| Project/Area Number |
23500505
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Meiji University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UMEYAMA Kazuhiro 明治大学, 研究 知財戦略機構, 准教授 (70342699)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ブタ / 遺伝子組換え動物 / 病態モデル動物 / 糖尿病 / 合併症 / 糖尿病腎症 / 糖尿病網膜症 |
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| Research Abstract |
This study aimed at identifying the characteristics of transgenic(tg) pigs as a research model for diabetes and its complications. Tg pigs carrying a mutant gene, human HNF-1alpha(P291fsinsC), responsible for maturity-onset diabetes of the young type 3, were obtained by in vitro fertilization using the cryopreserved sperm from tg founder. The tg pigs maintained a high blood glucose level(>200mg/dl). The oral glucose tolerance test showed that the recovery of the blood sugar levels of the pigs was significantly delayed compared with non-tg pigs. The hypoplasia of the islets of Langerhans was confirmed by the histopathological image of the tg pigs' pancreas. The histopathological image of the tg pigs' kidney showed a nodular lesion typical of diabetic nephropathy in humans. Also, retinal hemorrhages and cotton-wool spots were confirmed in the tg pigs. These data demonstrate that the tg pigs is a promising model for research into diabetets and its complications.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by <i>In Vitro</i> Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs2013
Author(s)
Umeyama K, Honda K, Matsunari H, Nakano K, Hidaka T, Sekiguchi K, Mochizuki H, Takeuchi Y, Fujiwara T, Watanabe M, Nagaya M, Nagashima H.
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Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 6
Pages: 599-603
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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[Presentation] ガラス化保存された体外成熟/体外受精胚を用いた糖尿病モデル遺伝子改変ブタの作出2013
Author(s)
松村幸奈, 前原美樹, 本田香澄, 林田豪太, 倉本桃子, 中野和明, 松成ひとみ, 小林美里奈, 内倉鮎子, 浅野吉則, 渡邊將人, 梅山一大, 長屋昌樹, 長嶋比呂志
Organizer
第106回日本繁殖生物学会大会
Place of Presentation
東京農工大学(東京都府中市)
Related Report
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[Presentation] 糖尿病モデル・変異型ヒト HNF-1α遺伝子を導入したトランスジェニックブタの病態解析2011
Author(s)
梅山一大, 渡邊將人, 松成ひとみ, 中野和明, 藤原主, 日高龍路, 竹内靖浩, 本田香澄, 望月寛徳, 関口渓人, 長嶋比呂志
Organizer
第25回日本糖尿病・肥満動物学会
Place of Presentation
東京大学(東京都文京区)
Year and Date
2011-11-05
Related Report
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