Importance of intestinal bacteria and dietary habits to maintain human health

• Project/Area Number: 23500958
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: The University of Tokushima
• Principal Investigator: KATAOKA Keiko 徳島大学, ヘルスバイオサイエンス研究部, 教授 (40189303)
• Co-Investigator(Kenkyū-buntansha): ARIMOCHI Hideki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (30311822)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腸内菌 / 無菌マウス / ノトバイオートマウス / B細胞 / インターロイキンー１０ / インターロイキンー２２ / 大腸炎モデル / インターロイキン-10

Research Abstract

We searched intestinal bacteria which can induce interleukin-10-producing cells. B cell-like cell population in mucosa of mouse small intestine was very little under germ free condition, but increased age-dependently or by administration of SPF mice feces. This cell population was decreased by antibiotics for Gram-positive bacteria, and increased in mice monoassociated with Enterococcus, an Gram-positive bacteria. This population additionally produced interleukin-22 which can mediate the production of antibacterial peptide and tissue repair. Partially purified fraction of this population showed inhibitory effect on colitis in naive T cell-transferred mice, and the effect was cancelled by injection of anti-interleukin-10 antibody.

Research Products

• [Journal Article] CD98hc regulates the development of experimental colitis by controlling effector and regulatory CD4+T cells. (2014)
  • Author(s): Bhuyan ZA, Arimochi H, Nishida J, Kataoka K, Kurihara T, Ishifune C, Tsumura H, Ito M, Ito Y, Kitamura A, Yasutomo K.
  • Journal Title: Biochemical Biophysical Research Communicatins Volume: 444(4) Issue: 4 Pages: 628-633
  • DOI: 10.1016/j.bbrc.2014.01.144
  • Peer Reviewed
• [Journal Article] The ARNT-STAT axis regulates the differentiation of intestinal intraepithelial TCRαβ+CD8αα+cells. (2013)
  • Author(s): Nakajima K, Maekawa K, Kataoka K, Ishifune C, Nishida J, Arimochi H, Yoshimoto T, Tomita S, Nagahiro S, Yasutomo K.
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1-11
  • DOI: 10.1038/ncomms3112
  • Peer Reviewed
• [Journal Article] Notch2 regulates the development of marginal zone B cells through Fos (2012)
  • Author(s): Iwahashi S, Maekawa Y, Nishida J, Ishifune C, Kitamura A, Arimochi H, Kataoko K, Chiba S, Shimada M, Yasutomo K
  • Journal Title: iochem Biophys Res Commun Volume: 418 Issue: 4 Pages: 701-707
  • DOI: 10.1016/j.bbrc.2012.01.082
  • Peer Reviewed
• [Journal Article] Reduced diversity and imbalance of fecal microbiota in patients with ulcerative colitis (2012)
  • Author(s): Nemoto H, Kataoka K, Ishikawa H, Ikata K, Arimochi H, Iwasaki T, Ohnishi Y, Kuwahara T, Yasutomo K
  • Journal Title: Digestive Diseases and Science Volume: Vol 57 Issue: 11 Pages: 2955-2964
  • DOI: 10.1007/s10620-012-2236-y
  • Peer Reviewed
• [Presentation] 日本免疫学会総会 (2011)
  • Author(s): 石舟智恵子, 他
  • Organizer: Notch シグナルは小腸粘膜固有層に存在するCD4+CD11c+細胞の分化に必要である
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-27
• [Presentation] Notchシグナルは小腸粘膜固有層に存在するCD4+CD11ｃ＋細胞の分化に必要である (2011)
  • Author(s): 石舟智恵子
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 幕張メッセ(千葉県）