Studies on genes relating to the formation of the testis-ovum in the testis of amphibians by the non-estrogenic action of environmental chemicals

• Project/Area Number: 23510077
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Hiroshima University
• Principal Investigator: TAKASE Minoru 広島大学, 理学(系)研究科(研究院), 准教授 (80226779)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 精巣卵 / 両生類 / 性転換 / 性分化 / 生殖腺 / ビスフェノールＡ / ノニルフェノール / ビスフェノールA / testis-ovum / frog / sex reversal / sex differentiation / gonad

Research Abstract

In the present study, the formation of the testis-ovum has been investigated in amphibians exposed to environmental chemicals, such as bisphenol A (BPA), nonylphenol (NP), and estradiol benzoate (EB). Firstly, exposure experiments were performed using three species of amphibians. Abnormal testes were observed more remarkably in the tadpoles and metamorphosed froglets of the frog Rana rugosa than in those of the other species. Thus, R. rugosa was used as a model of an experimental animal in further experiments. Next, the testes were analyzed histologically in the tadpoles exposed to environmental chemicals to know the process of testis-ovum formation. Finally, genes expressed differentially in the tadpoles exposed to BPA were analyzed during the formation of the testis-ova. Genes encoding vitellogenin A1 and ERRgamma were isolated as candidates relating to the formation of testis-ova induced by the non-estrogenic action of the environmental chemical.

Research Products

• [Journal Article] Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors (2013)
  • Author(s): Oka, T., Mitsui-Watanabe, N., Tatarazako, N., Onishi, Y., Katsu, Y., Miyagawa, S., Ogino, Y., Yatsu, R., Kohno, S., Takase, M., Kawashima, Y., Ohta, Y., Aoki, Y., Guillette, L. J. Jr., Iguchi, T
  • Journal Title: J. Appl. Toxicol Volume: 33 Pages: 991-1000
  • Peer Reviewed
• [Journal Article] Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors (2013)
  • Author(s): Oka, T., Mitsui-Watanabe, N., Tatarazako, N., Onishi, Y., Katsu, Y., Miyagawa, S., Ogino, Y., Yatsu, R., Kohno, S., Takase, M., Kawashima, Y., Aoki, Y., Guillette, L.J.Jr. and Iguchi, T.
  • Journal Title: J. Appl. Toxicol Volume: 33 Issue: 9 Pages: 991-1000
  • DOI: 10.1002/jat.2825
  • Peer Reviewed
• [Journal Article] Accumulation and pharmacokinetics of estrogenic chemicals in the pre-and post-hatch embryos of the frog Rana rugosa (2012)
  • Author(s): Takase, M., Shinto H., Takao, Y., Iguchi, T
  • Journal Title: In Vivo Volume: 26 Pages: 913-920
  • Peer Reviewed
• [Journal Article] Generation of albino Xenopus tropicals using zinc-finger nuclease (2012)
  • Author(s): Nakajima, K., Nakajima, T., Takase, M., Yaoita, Y
  • Journal Title: Dev. Growth Differ Volume: 54 Pages: 777-784
  • Peer Reviewed
• [Journal Article] Accumulation and pharmacokinetics of estrogenic chemicals in the pre- and post-hatch embryos of the frog Rana rugosa. (2012)
  • Author(s): Takase, M., Shinto, H., Takao, Y. and Iguchi, T.
  • Journal Title: In Vivo Volume: 26 Pages: 913-920
  • Peer Reviewed
• [Journal Article] Generation of albino Xenopus tropicalis using zinc-finger nucleases. (2012)
  • Author(s): Nakajima, K., Nakajima, T., Takase, M. and Yaoita, Y.
  • Journal Title: Dev. Growth Differ. Volume: 54 Pages: 777-784
  • NAID: 40019526018
  • Peer Reviewed
• [Presentation] Sex reversibility in amphibian and its applications : sex-hormone-inducible sex reversal in Rana rugosa, sex determination in Silurana tropicalis, and model amphibians as environmental indicators (2014)
  • Author(s): Takase, M
  • Organizer: International Symposium Frontiers in Amphibian Biology : Endangered Species Conservation and Genome Editing
  • Place of Presentation: Hiroshima, Japan
• [Presentation] トノサマガエルの生殖腺分化におけるエチニルエストラジオールの影響 (2013)
  • Author(s): 小林 亨,高瀬 稔,熊倉雅彦,山本 潤,長坂洋光,大西悠太,井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京
  • Year and Date: 2013-12-12
• [Presentation] 甲状腺ホルモンにより誘導されるネッタイツメガエル甲状腺ホルモン受容体遺伝子発現に対する化学物質抑制作用の肝臓と尾における違い (2013)
  • Author(s): 高瀬 稔,田澤一朗,矢尾板芳郞,井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京
  • Year and Date: 2013-12-12
• [Presentation] トノサマガエルの生殖腺分化におけるエチニルエストラジオールの影響 (2013)
  • Author(s): 小林 亨、高瀬 稔、熊倉雅彦、山本 潤、長坂洋光、大西悠太、井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学山上会館
• [Presentation] 甲状腺ホルモンにより誘導されるネッタイツメガエル甲状腺ホルモン受容体遺伝子発現に対する化学物質抑制作用の肝臓と尾における違い (2013)
  • Author(s): 高瀬 稔、田澤一朗、矢尾板芳郞、井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学山上会館
• [Presentation] 性分化後のツチガエルおよびトノサマガエルの生殖腺に対するノニルフェノールおよびビスフェノールA の影響 (2012)
  • Author(s): 高瀬 稔,井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京
  • Year and Date: 2012-12-19
• [Presentation] 性分化後のツチガエルおよびトノサマガエルの生殖腺に対するノニルフェノールおよびビスフェノールAの影響 (2012)
  • Author(s): 高瀬 稔、井口泰泉
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学山上会館
• [Presentation] ツチガエル性転換過程におけるLMO2およびLMO4の遺伝子発現 (2011)
  • Author(s): 高瀬 稔,井口泰泉
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ツチガエルの2集団間雑種へのアンドロゲン投与による性転換の誘導 (2011)
  • Author(s): 高瀬 稔
  • Organizer: 日本動物学会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] ツチガエルの2集団間雑種へのアンドロゲン投与による性転換の誘導 (2011)
  • Author(s): 高瀬 稔
  • Organizer: 日本動物学会
  • Place of Presentation: 旭川市大雪アリーナ
• [Presentation] ツチガエル性転換過程におけるＬＭＯ２およびＬＭＯ４の遺伝子発現 (2011)
  • Author(s): 高瀬 稔、井口泰泉
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市パシフィコ横浜