| Project/Area Number |
23510272
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kazusa DNA Research Institute |
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Principal Investigator |
SUZUKI Hideyuki 公益財団法人かずさＤＮＡ研究所, 産業基盤開発研究部, 主席研究員 (80276162)
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| Co-Investigator(Renkei-kenkyūsha) |
OGATA Yoshiyuki 大阪府立大学, 大学院生命環境科学研究科, 准教授 (90446542)
AKASHI Tomoyoshi 日本大学, 生物資源科学部, 講師 (80328707)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子共発現ネットワーク解析 / EST解析 / DNAマイクロアレイ / 次世代シークエンサー / 転写因子 / アヤメ科植物 / イソフラボノイド生合成 / 不定根培養 / 遺伝子共発現ネットワーク / DNA マイクロアレイ |
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| Research Abstract |
In order to isolate transcription factors involved in isoflavonoid biosynthesis, we established an EST (expressed sequence tag) data in Iris germanica root cultures, accumulating isoflavonoid, using next-generation sequencing instrument illumina HiSeq 1000 with 100 bp paired-end reads. Furthermore, we carrying out transcript expression analysis (using a custom Iris DNA array) in subculturing time-course experiment of Iris germanica root cultured cells for gene discovery in gene co-expression network analysis.
We established standalone Confeito Java-GUI (gene co-expression network analysis) software that extracted highly-interconnected gene modules. Using the public Arabidopsis DNA array resource data (more than 9 thousands), we have shown an application of our developed Confeito Java-GUI software to isolate gene co-expression network module in Arabidopsis aliphatic glucosinolate biosynthesis.
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