| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
Cryopreservation of spermatozoa requires elaborate protocols of freezing, the regulation of transport for frozen spermatozoa and addition of LN2 during storage. In this study, we report the fertilizing and developmental abilities of spermatozoa vacuum-dried with the stock solution (Tris-EGTA: TE) containing trehalose (Tr) and epicatechin (Asc), and then stored in the desiccator for 1 week at room temperature. Rehydrated spermatozoa were injected into oocytes using ICSI and embryonic development was followed. The percentages of blastocysts that developed from spermatozoa stored with Tr/TE, Tr+EC+Asc/TE, EC+Asc/TE and TE was significantly higher than spermatozoa without Tr. Two-cell embryos from spermatozoa stored with Tr+EC+Asc/TE for 446 days at room temperature developed to normal live offspring. These results indicate that Tr has a significant beneficial effect in preserving the developmental potential of mouse spermatozoa desiccated and stored at room temperature for long term.
|
