Regulation of Mphase progression by the SCFCdc4 ubiquitin ligase.
| Project/Area Number |
23570001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ゲノム構築・再編・維持 / 細胞周期 / 染色体分離 / ユビキチンリガーゼ / 転写 / M期終了 / 転写制御 / サイクリン / フィードバック阻害 |
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| Outline of Final Research Achievements |
Swi5 is a transcription factor that activates transcription of genes required for G1 functions in yeast. We previously showed that Swi5 is degraded by the SCFCdc4 ubiquitin ligase. In this study, we constructed a stabilized Swi5 by modifying residues required for the SCFCdc4-dependent degradation. We found that onset of S-phase, chrosomome segregation, and M-phase exit are inhibited in cells expressing the stabilized Swi5. We found a novel regulatory mechanism of cell cycle: Swi5 induces expression of Sic1 that inhibits initiation of S phase and chromosome segregation, and of Amn1 that inhibits M-phase exit; and degradation of Swi5 by the SCFCdc4 ubiquitin ligase allows proper progression into cell cycle.
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Report
(4 results)
Research Products
(1 results)
