| Project/Area Number |
23570086
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphology/Structure
|
|---|
| Research Institution | National Cardiovascular Center Research Institute |
|---|
Principal Investigator |
KAIYA Hiroyuki 独立行政法人国立循環器病研究センター, 研究所, 室長 (40300975)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIYAZATO Mikiya 独立行政法人 国立循環器病研究センター, 研究所, 部長 (50291183)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KANGAWA Kenji 独立行政法人 国立循環器病研究センター, 研究所, 所長 (00112417)
UCHIYAMA Minoru 富山大学, 理工学部, 教授 (50095072)
MATSUDA Kouhei 富山大学, 理工学部, 教授 (60222303)
KONNO Norifumi 富山大学, 理工学部, 助教 (50507051)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | グレリン / GHS-R / 無尾両生類 / 有尾両生類 / 機能解析 / 遺伝子発現部位 / 腹腔投与 / 摂食調節 / 両生類 / グレリン受容体 / セリン / スレオニン / アカハライモリ / ヒキガエル / リガンド選択性 / 受容体 / ウシガエル / アマガエル / 下垂体 |
|---|
| Research Abstract |
In this study, we identified ghrelin receptors (GHS-R1a) in three species of Anura (bullfrog, Japanese tree frog, and Japanese toad) and one species of Caudata (Japanese fire-belly newt). Functional analyses revealed that ghrelin receptors for Anura did not recognize serine or threonine at the third amino acid of ghrelin, but that of newt showed a high affinity for ghrelin with serine. We examined the effect of ghrelin on feeding regulation after intraperitoneal injection. Homologous ghrelin had no effect in newt, but increased food intake in bullfrog larvae. However, a fasting affects expressions of ghrelin and GHS-R1a genes in newt. Overall, it is suggested that the ghrelin system participates in energy homeostasis in amphibians. Our findings provide new evidences to ghrelin function in vertebrates.
|
