Mechanisms for regulation of bacterial gene to express pathogenicity and excape phagocytic killing in host
| Project/Area Number |
23570160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 細胞貪食 / 自然免疫 / 感染症 / 微生物 / マクロファージ / 食細胞 / 細菌 / 貪食 / 遺伝子発現 / ショウジョウバエ |
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| Research Abstract |
In infectious conditions, both host and bacteria sense each other and alter gene expression. We hypothesized that bacteria change gene transcription pattern to adapt to environmental changes and coexist with host. In this study, we found that lack of bacterial cell wall components induced persistence of bacterial infection by inhibition of phagocytosis by Drosophila macrophages. We observed enhanced expression of bacteria genes coding the sigma factors, a type of subunit of RNA polymerase, and factors of the two-component gene expression regulatory system upon bacterial phagocytosis by macrophages. We characterized these factors in influence of bacterial pathogenicity and found that some of them acted for inhibition of bacterial virulence and persistence in the host.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] ole for sigma38 in prolonged survival of Escherichia coli in Drosophila melanogaster.2014
Author(s)
Shiratsuchi,A., Shimamoto, N., Nitta, M., Tuan, T. Q., Firdausi, A., Gawasawa, M., Yamamoto, Y., Ishihama, A., and Nakanishi, Y.
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Journal Title
J. Immunol.
Volume: 192
Pages: 666-675
Related Report
Peer Reviewed
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[Journal Article] Involvement of EnvZ-OmpR two-component system in virulence control of Escherichia coli in Drosophila melanogaster.2013
Author(s)
Pullkay, P., Nakanishi, Y, Nitta, M., Yamamoto, K., Ishihama, A., and Shiratsuchi, A.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 438
Pages: 306-311
DOI
Related Report
Peer Reviewed
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[Journal Article] Phosphatidylserine recognition and induction of apoptotic cell clearance by Drosophila engulfment receptor Draper2013
Author(s)
Tung, T. T., Nagaosa, K., Fujita, Y., Kita, A., Mori, H., Okada, R., Nonaka, S., Nakanishi, Y
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Journal Title
J. Biochem.
Volume: 153
Pages: 483-491
DOI
Related Report
Peer Reviewed
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[Journal Article] Independent recognition of Staphylococcus aureus by two receptors for phagocytosis in Drosophila2012
Author(s)
Shiratsuchi, A., Mori, T., Sakurai, K., Nagaosa, K., Sekimizu, K., Lee, B. L., Nakanishi, Y
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Journal Title
J . Biol. Chem.
Volume: 287
Pages: 21663-21672
DOI
Related Report
Peer Reviewed
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[Journal Article] Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila2012
Author(s)
Okada, R., Nagaosa, K., Kuraishi, T., Nakayama, H., Yamamoto, N., Nakagawa, Y., Dohmae, N., Shiratsuchi, A., Nakanishi, Y
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Journal Title
J Biol Chem
Volume: 287
Pages: 3138-3146
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] 93-kDa twin-domain serine protease inhibitor (serpin) has a regulatory function of the beetle Toll proteolytic signaling cascade.2011
Author(s)
Jiang, R., Zhang, B., Kurokawa, K., So, Y.-I., Kim, E.-H., Hwang, H. O., Lee, J.-H., Shiratsuchi, A., Zhang, J., Nakanishi, Y., Lee, H.-S., and Lee, B.L.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 35087-3509
DOI
Related Report
Peer Reviewed
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