Functional crosstalk between ARF and Rab small GTPases

• Project/Area Number: 23570162
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: SHIN Hye-Won 京都大学, 薬学研究科(研究院), 准教授 (10345598)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: メンブレントラフィック / 小胞輸送 / エンドソーム / 生体膜 / Gタンパク質 / リサイクリング / 低分子量GTPase / 細胞 / タンパク質

Research Abstract

Small GTPases of the ARF family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. I found that the simultaneous depletion of ARF1/ARF3 or ARF1/ARF4 induced extensive tabulation of Rab4- and Rab11-positive recycling endosomes. Moreover, ARF1/ARF3 are redundantly required for recycling pathway from the recycling endosomes to the plasma membrane. On the other hand, ARF1/ARF4 are redundantly required for the retrograde transport of TGN38 and mannose-6-phosphate receptor from recycling endosomes to the the trans-Golgi network (TGN), but was not required for the recycling pathway. It remains important to determine how distinct combinations of ARFs sort cargos targeted to distinct organelles and regulate separate transport processes on the same compartments. ARFs may act within distinct membrane microdomains along with distinct effector proteins.

Research Products

• [Journal Article] ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus (2013)
  • Author(s): Nakai, W., Kondo, Y., Saitoh, A., Naito, T., Nakayama, K., and Shin, H.-W.
  • Journal Title: Mol. Biol. Cell Volume: 24 Issue: 16 Pages: 2570-2581
  • DOI: 10.1091/mbc.e13-04-0197
  • NAID: 120005324911
  • Peer Reviewed
• [Journal Article] EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis (2013)
  • Author(s): Ueda, T., Hanai, A, Takei, T., Kubo, K., Ohgi, M., Sakagami, H., Takahashi, S., Shin, H.-W., and Nakayama, K.
  • Journal Title: FEBS Lett. Volume: 587 Pages: 1617-1623
  • Peer Reviewed
• [Journal Article] Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes (2013)
  • Author(s): Takatsu, H., Katoh, Y., Ueda, T., Waguri, S., Murayama, T., Takahashi, S., Shin, H.-W. &Nakayama, K.
  • Journal Title: Cell Structure and Function Volume: 38 Issue: 1 Pages: 31-41
  • DOI: 10.1247/csf.12028
  • NAID: 130004053889
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] EFA6 locally activates Arf6 and targets it to the Flemming body during cytokinesis (2013)
  • Author(s): Tomoko Ueda, Ayako Hanai, Tomomi Takei, Keiji Kubo, Minako Ohgi, Hiroyuki Sakagami, Senye Takahashi, Hye-Won Shin, Kazuhisa Nakayama
  • Journal Title: FEBS Letters Volume: in press
  • Peer Reviewed
• [Journal Article] ARF1 and ARF3 Are Required for the Integrity of Recycling Endosomes and the Recycling Pathway (2012)
  • Author(s): Kondo, Y., Hanai, A., Nakai, W., Katoh, Y.,Nakayama, K. & Shin, H.-W.
  • Journal Title: Cell Structure and Function Volume: 37 Issue: 2 Pages: 141-154
  • DOI: 10.1247/csf.12015
  • NAID: 130004137585
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Mechanisms of membrane curvature generation in membrane traffic (2012)
  • Author(s): Shin, H.-W., Takatsu, H., and Nakayama, K.
  • Journal Title: Membranes Volume: 2 Issue: 1 Pages: 118-133
  • DOI: 10.3390/membranes2010118
  • NAID: 120004025602
  • Peer Reviewed
• [Journal Article] Structural basis for membrane binding specificity of the Bin/Amphiphysi n/Rvs (BAB) domain of Arfaptin-2 determined by Aril GTPase. (2012)
  • Author(s): Nakamura, et al
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 30 Pages: 25478-25489
  • DOI: 10.1074/jbc.m112.365783
  • Peer Reviewed
• [Journal Article] ゴルジ体以降の小胞輸送における低分子量GTPaseとBARドメインタンパク質の役割 (2012)
  • Author(s): 申惠媛、満智秋、中山和久.
  • Journal Title: 生化学 Volume: 84 Pages: 785-790
  • NAID: 10031122385
• [Journal Article] Rab11 regulates exocytosis of recycling vesicles at the plasma membrane. (2012)
  • Author(s): Takahashi, S., Kubo, K., Waguri, S., Yabashi, A., Shin, H.-W., Katoh, Y. & Nakayama, K.
  • Journal Title: J. Cell Sci., 1 Volume: 125 Pages: 4049-4057
  • DOI: 10.1242/jcs.102913
  • Peer Reviewed
• [Journal Article] Structural basis for Arf6-MKLP1complex formation on the Flemming body responsible for cytokinesis. (2012)
  • Author(s): Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.-W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S. & Nakayama, K.
  • Journal Title: EMBO J Volume: 31 Issue: 11 Pages: 2590-2603
  • DOI: 10.1038/emboj.2012.89
  • NAID: 120004161052
  • Peer Reviewed
• [Journal Article] ゴルジ体以降の小胞輸送における低分子量GTPaseとBARドメインタンパク質の役割. (2012)
  • Author(s): 申 惠媛、満 智秋、中山 和久
  • Journal Title: 生化学 Volume: 84 Pages: 785-790
  • NAID: 10031122385
• [Journal Article] GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER Transport Involved in Regulation of Lipid Homeostasis (2011)
  • Author(s): Takashima, K., Saitoh, A., Hirose, S., Nakai, W., Kondo, Y., Takasu, Y., Kakeya, H., Shin, H.-W. & Nakayama, K8.
  • Journal Title: Cell Structure and Function Volume: 36 Issue: 2 Pages: 223-235
  • DOI: 10.1247/csf.11035
  • NAID: 130004137571
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50-independent manner. (2011)
  • Author(s): Takatsu, H., Baba, K., Shima, T., Umino, H., Kato, U., Umeda, M., Nakayama, K. & Shin, H.-W.
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 44 Pages: 38159-38167
  • DOI: 10.1074/jbc.m111.281006
  • Peer Reviewed
• [Journal Article] Distinct roles of Rab11 and Arf6 in the regulation of Rab11-FIP3/Arfophilin-1 localization in mitotic cells (2011)
  • Author(s): Takahashi, S., Takei, T., Koga, H., Takatsu,H., Shin, H.-W. & Nakayama, K.
  • Journal Title: Genes Cells Volume: 16 Issue: 9 Pages: 938-950
  • DOI: 10.1111/j.1365-2443.2011.01538.x
  • Peer Reviewed
• [Journal Article] Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs. (2011)
  • Author(s): Man, Z., Kondo, Y., Koga, H., Umino, H., Nakayama, K. & Shin, H.-W.
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 13 Pages: 11569-11578
  • DOI: 10.1074/jbc.m110.201442
  • Peer Reviewed
• [Presentation] リサイクリングエンドソームを介する輸送経路におけるタンパク質とリン脂質の協調的作用 (2013)
  • Author(s): 申惠媛
  • Organizer: 第86回日本生化学会大会.シンポジウム.
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Membrane remodeling in post-Golgi trafficking (2013)
  • Author(s): 申惠媛
  • Organizer: 第86回日本生化学会大会.シンポジウム.
  • Place of Presentation: 名古屋(ウインクあいち)
  • Invited
• [Presentation] リサイクリングエンドソームを介する輸送経路におけるタンパク質とりん脂質の強調的作用 (2013)
  • Author(s): 申 惠媛
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] Membrane remodeling in post-Golgi trafficking (2013)
  • Author(s): 申 惠媛
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋（ウインクあいち）
  • Invited
• [Presentation] Membrane remodeling in post-Golgi trafficking (2013)
  • Author(s): 申 惠媛
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち（愛知県）
  • Invited
• [Presentation] メンブレントラフィックにおけるリン脂質フリッパーゼの機能 (2013)
  • Author(s): 申 惠媛
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] 生体膜の非対称性を制御するP4-ATPaseの小胞輸送における機能 (2012)
  • Author(s): 申惠媛
  • Organizer: 第34回生体膜の薬物の相互作用シンポジウム
  • Place of Presentation: 京都(京大)
• [Presentation] 生体膜の脂質非対称性を調節するP4-ATPaseのゴルジ体以降の小胞輸送経路における機能解析 (2012)
  • Author(s): 海野寛之
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 生体膜の非対称性を制御するP4-ATPaseの小胞輸送における機能 (2012)
  • Author(s): 申 惠媛
  • Organizer: 第34回生体膜の薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部（京都府）
• [Presentation] 生体膜の脂質非対称性を調節するP4-ATPaseのゴルジ体以降の商法輸送における機能解析 (2012)
  • Author(s): 海野 寛之
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] ゴルジ体に局在する低分子量GTPase ARFの機能の再考 (2011)
  • Author(s): 申惠媛
  • Organizer: 第84回日本生化学会大会シンポジウム
  • Place of Presentation: 京都
  • Invited
• [Presentation] メンブレントラフィックにおけるP4-ATPase、ATP9Bの機能解析 (2011)
  • Author(s): 高津宏之
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] 脂質二重層の非対称性を調節するP4-ATPaseのメンブレントラフィックにおける役割 (2011)
  • Author(s): 島孝弘
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] ゴルジ体‐小胞体間のタンパク質輸送による脂肪滴形成の調節 (2011)
  • Author(s): 高島昭平
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Functional analysis of class I and classII ARF small GTPases in membrane traffic (2011)
  • Author(s): 中井和香
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
• [Presentation] Regulation of lipid droplet formation by Golgi-to-ER membrane traffic (2011)
  • Author(s): 高島皓平
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
• [Presentation] Arfaptins localize to the trans-Golgi in an Arl1-dependent manner and deform Golgi membranes (2011)
  • Author(s): 満智秋
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
• [Presentation] ゴルジ体に局在する低分子量GTPase ARFの機能の再考 (2011)
  • Author(s): 申 惠媛
  • Organizer: 第84回日本生化学会シンポジウム(招待講演)
  • Place of Presentation: 京都国際会館
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/hshin/ShinIndex.html