| Project/Area Number |
23570219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
OGAWA Hidesato 独立行政法人情報通信研究機構, 未来ICT研究所 バイオICT研究室, 主任研究員 (20370132)
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| Co-Investigator(Renkei-kenkyūsha) |
MOROHASHI Ken-ichirou 九州大学, 医学研究院, 教授 (30183114)
TAKEUCHI Jun 東京大学, 分子細胞生物学研究所, 准教授 (10451999)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 転写 / クロマチン / 核内受容体 / 発現制御 / 核内構造体 / 転写因子 / 翻訳後修飾 / 核構造 |
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| Research Abstract |
For understanding on the molecular processes of development, cell growth and homeostasis, it is required to reveal a regulation mechanism of binding of the transcription factors to the target genes. One of a useful method to introduce a new insight into the complex formation with the target DNA is to visualize the processes using the cell line that possesses a tandem repeat array of the mouse mammary tumor virus (MMTV) promoter that is the target of the steroid receptor (GR, glucocorticoid receptor). Using this system, we succeeded in finding of a novel complex formation on the MMTV array. Although the chromatin-remodeling factor, ARIP4, is localized on nuclear structure without the glucocorticoid, after addition of the ligand, ARIP4 is co-localized with GR on the target DNA. This suggests that the MMTV array system is useful to analyze an important role of nuclear structure in the regulation of nuclear transcriptional complex formation.
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