Regulation of cellular functions by highly evolved two chaperonins

• Project/Area Number: 23570220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Akita University
• Principal Investigator: KUBOTA HIROSHI 秋田大学, 工学(系)研究科(研究院), 教授 (80332724)
• Co-Investigator(Renkei-kenkyūsha): NAGATA Kazuhiro 京都産業大学, 総合生命科学部, 教授 (50127114) ; KINJO Masataka 北海道大学, 先端生命科学研究院, 教授 (70177971) ; KITAMURA Akira 北海道大学, 先端生命科学研究院, 助教 (10580152)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子シャペロン / フォールディング / 複合体形成 / ATPase / CCT / HSP60 / 反応サイクル / タンパク質相互作用 / 構造変化 / 機能制御 / 基質特異性 / ATP

Research Abstract

Chaperonins are a group of molecular chaperone that assists in protein folding in the cell. In this project, we analyzed two eukaryotic chaperonins: cytosolic chaperonin CCT and mitochondrial chaperonin HSP60. In the study of CCT, we found that CCT possesses GTPase activity in addition to the ATPase activity. In the study of HSP60, we found that football-like complex plays an important role in the function of HSP60 after formation of the football-like complex from single-ring complex via double-ring complex. These results contain important findings to understand chaperonin functions.

Research Products

• [Journal Article] ATPase activity and ATP-dependent conformational change in the co-chaperone HOP. (2014)
  • Author(s): Yamamoto S, Subedi GP, Hanashima S, Satoh T, Otaka M, Wakui H, Sawada KI, Yokota SI, Yamaguchi Y, Kubota H, Itoh H.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 14 Pages: 9880-9886
  • DOI: 10.1074/jbc.m114.553255
  • Peer Reviewed
• [Journal Article] Serum heat shock protein 47 levels are elevated in acute interstitial pneumonia. (2014)
  • Author(s): Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi, T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
  • Journal Title: BMC Pulm. Med. Volume: 14 Issue: 1 Pages: 48-48
  • DOI: 10.1186/1471-2466-14-48
  • NAID: 120006986596
  • Peer Reviewed
• [Journal Article] Serum heat shock protein 47 levels are elevated in acute exacerbation of idiopathic pulmonary fibrosis (2013)
  • Author(s): Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi. T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
  • Journal Title: Cell Stress Chaperones Volume: 18 Issue: 5 Pages: 581-590
  • DOI: 10.1007/s12192-013-0411-5
  • Peer Reviewed
• [Journal Article] Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation. (2013)
  • Author(s): Tashiro E, Zako T, Muto H, Itoo Y, Sorgjerd K, Terada N, Abe A, Miyazawa M, Kitamura A, Kitaura H, Kubota H, Maeda M, Momoi T, Iguchi-Ariga SM, Kinjo M, Ariga H.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 27 Pages: 19958-19972
  • DOI: 10.1074/jbc.m113.477984
  • NAID: 120005323603
  • Peer Reviewed
• [Journal Article] Serum heat shock protein 47 levels in patients with drug-induced lung disease. (2013)
  • Author(s): Kakugawa, T., Yokota, S., Ishimatsu, Y., Hayashi, T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Y., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
  • Journal Title: Respir. Res. Volume: 14 Issue: 1 Pages: 133-133
  • DOI: 10.1186/1465-9921-14-133
  • Peer Reviewed
• [Journal Article] Serum heat shock protein 47 levels are elevated in acute exacerbation of idiopathic pulmonary fibrosis. (2013)
  • Author(s): Kakugawa, T., Yokota, S.I., Ishimatsu, Y., Hayashi. T., Nakashima, S., Hara, S., Sakamoto, N., Kubota, H., Mine, M., Matsuoka, Y., Mukae, H., Nagata, K., and Kohno, S.
  • Journal Title: Cell Stress Chaperones Volume: 未定
  • Peer Reviewed
• [Journal Article] Multipotent stem cells are effectively collected from adult human cheek skin. (2012)
  • Author(s): Yoshikawa, K., Naitoh, M., Kubota, H., Ishiko, T., Aya, R., Yamawaki, S. and Suzuki, S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 431 Issue: 1 Pages: 104-110
  • DOI: 10.1016/j.bbrc.2012.12.069
  • Peer Reviewed
• [Journal Article] Pirfenidone inhibits TGF-beta1-induced over-expression of collagen type I and heat shock protein 47 in A549 cells. (2012)
  • Author(s): Hisatomi, K., Mukae, H., Sakamoto, N., Ishimatsu, Y., Kakugawa, T., Hara, S., Fujita, H., Nakamichi, S., Oku, H., Urata, Y., Kubota, H., Nagata, K. and Kohno, S.
  • Journal Title: BMC Pulm. Med. Volume: 12 Issue: 1
  • DOI: 10.1186/1471-2466-12-24
  • Peer Reviewed
• [Journal Article] Cytosolic chaperonin CCT possesses GTPase activity (2011)
  • Author(s): Noguchi, et al
  • Journal Title: Am.J.Mol.Biol. Volume: 1 Issue: 03 Pages: 123-130
  • DOI: 10.4236/ajmb.2011.13013
  • Peer Reviewed
• [Journal Article] Protective role of the ubiquitin binding protein Tollip against the toxicity of polyglutamine-expansion proteins (2011)
  • Author(s): Oguro, et al
  • Journal Title: Neurosci.Lett. Volume: 503 Issue: 3 Pages: 234-239
  • DOI: 10.1016/j.neulet.2011.08.043
  • Peer Reviewed
• [Journal Article] Protective role of the ubiquitin binding protein Tollip against the toxicity of polyglutamine-expansion proteins (2011)
  • Author(s): Oguro et al.
  • Journal Title: Neurosci. Lett. Volume: 503 Pages: 234-239
  • Peer Reviewed
• [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD変異体のリソソーム依存的分解 (2013)
  • Author(s): 田村拓, 山田大介, 久保田広志
  • Organizer: 第8回臨床ストレス応答学会
  • Place of Presentation: 松本市
• [Presentation] Lysosome-dependent degradation of ALS-linked mutant SOD1 (2013)
  • Author(s): Daisuke Yamada, Taku Tamura, Hiroshi Kubota
  • Organizer: International Mini-symposium Protein Folding and Disease
  • Place of Presentation: 秋田市
• [Presentation] Actin and myosin participate in the quality control of SOD1 aggregates (2013)
  • Author(s): Taku Tamura, Akihiko Yatabe, Koji Ichinohe, Yuko Shimizu, Akira Kitamura, Masataka Kinjo, Hiroshi Kubota
  • Organizer: International Mini-symposium Protein Folding and Disease
  • Place of Presentation: 秋田市
• [Presentation] アクチン・ミオシン系は筋萎縮性側索硬化症原因タンパク質SOD1変異体の凝集制御および分解に関与する (2013)
  • Author(s): 田村拓, 矢田部暁彦, 一戸康治, 清水佑子, 北村朗, 金城政孝, 久保田広志
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋市
• [Presentation] Aminoglycoside antibiotics suppress the Heat Shock Protein 70 function (2013)
  • Author(s): Yamamoto, S., Yokota, S., Wakui, H., Kubota, H., Itoh, H, and Fujii, N.
  • Organizer: 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: 横浜市
• [Presentation] Aminoglycoside antibiotics suppress the Heat Shock Protein 70 function. (2013)
  • Author(s): Yamamoto S, Yokota S, Wakui H, Kubota H, Itoh H, Fujii N
  • Organizer: 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: 横浜市
• [Presentation] Lysosome-dependent degradation of ALS-linked mutant SOD1 (2013)
  • Author(s): Daisuke Yamada, Taku Tamura, Hiroshi Kubota
  • Organizer: International Mini-symposium: Protein Folding and Disease
  • Place of Presentation: 秋田市
• [Presentation] Actin and myosin participate in the quality control of SOD1 aggregates (2013)
  • Author(s): Taku Tamura, Akihiko Yatabe, Koji Ichinohe, Yuko Shimizu, Akira Kitamura, Masataka Kinjo, Hiroshi Kubota
  • Organizer: International Mini-symposium: Protein Folding and Disease
  • Place of Presentation: 秋田市
• [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD1変異体のリソソーム依存的分解 (2013)
  • Author(s): 田村拓、山田大介、久保田広志
  • Organizer: 第８回臨床ストレス応答学会
  • Place of Presentation: 松本市
• [Presentation] 筋萎縮性側索硬化症原因タンパク質SOD1変異体の凝集制御および分解おけるアクチンフィラメントの役割 (2012)
  • Author(s): 田村拓, 矢田部暁彦, 一戸康児, 清水佑子, 北村朗, 金城政孝, 久保田広志
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] Serine Protease-Family Protein HtrA1 is Specifically Up-regulated in Keloid Legions (2012)
  • Author(s): Motoko Naitoh, Toshihiro Ishiko, Satoko Yamawaki, Taku Tamura, Rino Aya, Katsuhiro Yoshikawa, Hiroshi Kubota and Shigehiko Suzuki.
  • Organizer: 第4回世界創傷治癒学会連合会議(WUWHS 2012)
  • Place of Presentation: 横浜市
• [Presentation] Serine Protease-Family Protein HtrA1 is Specifically Up-regulated in Keloid Legions (2012)
  • Author(s): Motoko Naitoh, Toshihiro Ishiko, Satoko Yamawaki, Taku Tamura, Rino Aya, Katsuhiro Yoshikawa, Hiroshi Kubota and Shigehiko Suzuki.
  • Organizer: 第4回世界創傷治癒学会連合会議（WUWHS 2012）
  • Place of Presentation: 横浜市
• [Presentation] シャペロニンHsp60のATP、Hsp10依存的なダブルリング化について (2011)
  • Author(s): 石田竜一, 久保田広志, 高橋弘樹, 岡俊, 北村朗, 金城政孝, 伊藤英晃
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪市
• [Presentation] シャペロニンHsp60 のATP、Hsp10 依存的なダブルリング化について (2011)
  • Author(s): 石田竜一 他
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪市