| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Research Abstract |
Spinach is widely known to be dioecious. However, monoecious plants can also occur in this species. Sex expression in dioecious spinach plants is controlled by a single gene pair termed X and Y. Our previous study showed that a single, incompletely dominant gene, which controls the monoecious condition, is linked to X/Y.
The four Y-linked AFLP markers developed in this study were converted into sequence-characterized amplified region (SCAR) markers. Linkage analysis using the four SCAR markers and three already-known Y-linked markers suggested that recombination is suppressed around the male-determining gene (Y). Analysis of spinach genomic BAC clones isolated with the Y-linked markers suggested that the male-specific (non-recombining) region is at least larger than 840 kbp. Further, most part of this chromosomal region was found to be accounted for by interspersed repeated elements, e.g. retroposons, while only a few protein-coding genes were identified in this region.
|
