Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Research Abstract |
White-flowered transgenic plants resulted from RNAi suppression of the CHS gene and dwarf transgenic plants resulted from overexpression of the GA2ox gene were selected as breeding materials through characterization of various transgenic plants of Tricyrtis spp. Interspecific hybrids were produced via ovule culture when dwarf transgenic plants were used as parents. Some of these hybrids showed not only dwarf phenotypes due to the transgene but also novel flower characteristics compared with the parents, indicating the possibility of interspecific hybridization using transgenic plants in the improvement programs of ornamental plants. On the other hand, induction of somaclonal variants and chromosome doubling were also examined by using embryogenic callus cultures of transgenic plants. Detailed characterization of callus culture-derived plants are now in progress.
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