| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Research Abstract |
Acetic acid fermentation by acetic acid bacteria is a typical microbial transformation from ethanol to acetic acid. The present study conducted genetic and biochemical analyses on aldehyde dehydrogenase (ALDH) catalyzing oxidation of acetaldehyde into acetic acid in Acetobacter pasteurianus, a member of acetic acid bacteria. It was shown that A. pasteurianus has two kinds of ALDH complexes: canonical one is essential for acetic acid fermentation, but paralogous one less contributes to that. Two ALDH complexes were different from each other as to pH optima and affinities to acetaldehyde. The paralogous ALDH was purified as complex consists of three subunits, but canonical ALDH seemed to be unstable and prone to be decomposed into subunit level.
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