Functions of sulfotransferases and their signal transductions
Project/Area Number |
23580138
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | University of Miyazaki |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SAKAKIBARA Yoichi 宮崎大学, 大学院農学工学総合研究科, 教授 (90295197)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | 酵素 / 硫酸転移酵素 / 薬物代謝 / 硫酸化 / プロスタグランジン / オーファン / ゼブラフィッシュ |
Research Abstract |
In this research project, we focused on the evaluation of physiological relevance of two kinds of orphan sulfotransferases (SULTs) such as SULT4A1 and SULT7A1 whose biochemical/physiological functions are unknown. RT-PCR and in situ hybridization analyses using zebrafish embryos revealed that SULT4A1 was specifically expressed in whole brain and spinal cord. These results indicate that SULT4A1 plays important roles in the early development of a nervous system of zebrafish embryos. On the other hand, detail characterization of mouse SULT7A1 demonstrated that SULT7A1 exhibited the specific sulfating activity toward cyclopentenone prostaglandins including PGA2 and 15-deoxy-delta-12,14-PGJ2 (15d-PGJ2), which were endogenous metabolites of PGE2 and PGD2, respectively, but not PGE2 and PGD2. These studies would encourage us to investigate additional biochemical characterization of these orphan SULTs in the pursuit of yet-to-be-defined function.
|
Report
(4 results)
Research Products
(77 results)
-
[Journal Article] Identification of a novel flavonoid glycoside sulfotransferase in Arabidopsis thaliana.2014
Author(s)
Hashiguchi, T., Sakakibara, Y, Shimohira, T., Kurogi, K, Yamasaki, M., Nishiyama, K., Akashi, R., Liu, M. ・C., Suiko, M.
-
Journal Title
The Journal of Biochemistry
Volume: 155
Issue: 2
Pages: 91-97
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Identification and characterization of a novel kaempferol sulfotransferase in Arabidoosis thaliana.2013
Author(s)
Hashiguchi, T., Sakakibara, Y., Hara Y., Shimohira, T., Kurogi, K., Akashi, R., Liu, M. -C., Suiko, M
-
Journal Title
Biochemical and Biophysical Research Communications
Volume: 434
Issue: 4
Pages: 829-835
DOI
Related Report
Peer Reviewed
-
[Journal Article] Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.2013
Author(s)
Teramoto, T., Fujikawa, Y., Kawaguchi, Y., Kurogi, K., Soejima, M., Adachi, R., Nakanishi, Y., Mishiro-Sato, E., Liu, M.-C., Sakakibara, Y., Suiko, M., Kimura, M. and Kakuta, Y.
-
Journal Title
Nature communication
Volume: 4
Issue: 1
Pages: 1572-1572
DOI
Related Report
Peer Reviewed
-
[Journal Article] Ethanol sulfation by the human cytosolic sulfotransferases : a systematic analysis2012
Author(s)
Kurogi, K., Davidson, G., Mohammed, Y.I., Williams, F.E., Liu, M.Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
-
Journal Title
Bio. Pharm. Bull
Volume: 35(12)
Pages: 2180-2185
NAID
Related Report
Peer Reviewed
-
[Journal Article] Sulfation of buprenorphine, pentazaocine, and naloxone by human cytosolic sulfotransferases2012
Author(s)
Kurogi, K., Chen, M., Lee, Y., Shi, B., Yang, T., Liu, M.Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
-
Journal Title
Drug Metab. Lett
Volume: 6(2)
Pages: 109-115
Related Report
Peer Reviewed
-
[Journal Article] Concerted actions of the catechol O-methyltransferase and the cytosolic sulfotransferase SULT1A3 in the metabolism of catecholic drugs2012
Author(s)
Kurogi, K., Alazizi, A., Liu, M.Y., Sakakibara, Y., Suiko, M., Sugahara, T. and Liu, M.-C.
-
Journal Title
Biochem. Pharmacol
Volume: 84
Issue: 9
Pages: 1186-1195
DOI
Related Report
Peer Reviewed
-
[Journal Article] Sulfation of ractopamine and salbutamol by the human cytosolic sulfotranasferases2012
Author(s)
Ko, K., Kurogi, K., Davidson, G., Liu, M.-Y., Sakakibara, Y., Suiko, M. and Liu, M.-C.
-
Journal Title
J . Biochem
Volume: 152(3)
Issue: 3
Pages: 275-283
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Sulfation of ractopamine and salbutamol by the human cytosolic sulfotranasferases.2012
Author(s)
Ko, K., Kurogi, K., Davidson, G., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
-
Journal Title
J. Biochem.
Volume: 152(3)
Pages: 275-283
Related Report
Peer Reviewed
-
[Journal Article] Concerted actions of the catechol O-methyltransferase and the cytosolic sulfotransferase SULT1A3 in the metabolism of catecholic drugs.2012
Author(s)
Kurogi, K., Alazizi, A., Liu, M.-Y., Sakakibara, Y., Suiko, M., Sugahara, T., Liu, M.-C.
-
Journal Title
Biochem. Pharmacol.
Volume: 84(9)
Pages: 1186-1195
Related Report
Peer Reviewed
-
[Journal Article] Sulfation of buprenorphine, pentazocine, and naloxone by human cytosolic sulfotransferases.2012
Author(s)
Kurogi, K., Chen, M., Lee, Y., Shi, B., Yang, T., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu M.-C.
-
Journal Title
Drug Metab. Lett.
Volume: 6(2)
Pages: 109-115
Related Report
Peer Reviewed
-
[Journal Article] Ethanol sulfation by the human cytosolic sulfotransferases: A systematic analysis.2012
Author(s)
Kurogi, K., Davidson, G., Mohammed, Y.I., Williams, F.E., Liu, M.Y., Sakakibara, Y., Suiko, M., and Liu, M.-C.
-
Journal Title
Biol. Pharm. Bull.
Volume: 35(12)
Pages: 2180-2185
NAID
Related Report
Peer Reviewed
-
[Journal Article] Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST52012
Author(s)
Mohammed, Y.I., Kurogi, K., Liu, M.-Y., Sakakibara, Y., Suiko, M., Liu, M.-C.
-
Journal Title
Aquat. Toxicol.
Volume: 112-113C
Pages: 11-18
Related Report
Peer Reviewed
-
[Journal Article] Enzymatic sulfation of tocopherols and tocopherol metabolites by human cytosolic sulfotransferases2011
Author(s)
Hashiguchi, T., Kurogi, K., Sakakibara, Y., Yamasaki, M., Nishiyama, K., Yasuda, S., Liu, M.-C. and Su iko, M.
-
Journal Title
Biosci. Biotechnol. Biochem
Volume: 75
Pages: 1951-1956
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Identification, characterization, and ontogenic study of a catechol O- methyltransferase from zebrafish2011
Author(s)
Alazizi, A., Kurogi, K., Sakakibara, Y., Suiko, M. and Liu, M.-C.
-
Journal Title
Aquat. Toxicol
Volume: 102
Issue: 1-2
Pages: 18-23
DOI
Related Report
Peer Reviewed
-
[Journal Article] Sulfation of chlorotyrosine and nitrotyrosine by human lung endothelial and epithelial cells : Role of the human SULT1A32011
Author(s)
Yasuda, S., Yasuda, T., Liu, M.-Y., Shetty, S., Idell, S., Boggaram, V., Suiko, M., Sakakibara, Y. and Liu, M.-C.
-
Journal Title
Toxicol. Appl. Pharmacol
Volume: 251(2)
Issue: 2
Pages: 104-109
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Identification, characterization, and ontogenic study of a catechol O-methyltransferase from zebrafish2011
Author(s)
Alazizi, A., Liu, M.-Y., Kurogi, K., Sakakibara, Y., Suiko, M., Liu, M.-C.
-
Journal Title
Aquat. Toxicol.
Volume: 102(1-2)
Pages: 18-23
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Two-dimensional gel electrophoresis analysis of fravonoid sulfate effects on A. thaliana T87 cells2013
Author(s)
Hashiguchi, T., Sakakibara, Y., Shimohira, T., Yoshimura, T., Kurogi, K., Hara, Y., Liu, M.-C., Suiko, M.
Organizer
The 12th Human Proteome Organisation World Congress (HUPO 2013)
Place of Presentation
Yokohama
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Two-dimensional gel electrophoresis analysis of fravonoid sulfate effects on A. thaliana T87 cells.2013
Author(s)
Hashiguchi, T., Sakakibara, Y., Shimohira, T., Yoshimura, T., Kurogi, K., Hara, Y., Liu, M.-C., Suiko, M.
Organizer
The 12th Human Proteome Organization World Congress (HUPO 2013)
Place of Presentation
Pacifico Yokohama
Related Report
-
-
[Presentation] Zebrafish tyrosylprotein sulfotransferase related genes: molecular cloning, and functional characterization2013
Author(s)
Mishiro-Sato, E., Kondo, T., Kenmochi, N., Uechi, T., Kurogi, K., Liu, M.-C., Suiko, M., Sakakibara, Y.
Organizer
The 12th Human Proteome Organization World Congress (HUPO 2013)
Place of Presentation
Pacifico Yokohama
Related Report
-
[Presentation] Crystal structure of tyrosylprotein sulfotransferase: the reaction mechanism of post-translational tyrosine sulfation.2013
Author(s)
Teramoto, T., Kurogi, K., Sakakibara, Liu, M.-C., Suiko, M., Kimura, M., Kakuta, Y.
Organizer
The 12th Human Proteome Organization World Congress (HUPO 2013)
Place of Presentation
Pacifico Yokohama
Related Report
-
-
-
-
-
[Presentation] Human tyrosylprotein sulfotransferase: Differential enzymatic characteristics and tissue-specific expression.2013
Author(s)
Suiko, M., Sakakibara, Y., Kakuta, Y., Liu, M.-C., Kimura, M.
Organizer
Proteomic Forum 2013 in Berlin
Place of Presentation
Berlin, Germany
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-