Molecular mechanism underlying the breakdown of chromosomal homeostasis by aberrant activation of cyclin A-CDK
| Project/Area Number |
23580140
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Tokyo University of Agriculture |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 細胞周期 / がん / サイクリンA / サイクリン依存性キナーゼ / Mcm7 / S期移行 / 染色体倍数化 / 中心体複製 / Mps1 |
|---|
| Research Abstract |
Aberrant activation of cyclin A (CycA)-CDK complex, one of the central regulatory factors for mammalian cell cycle progression, leads to the acceleration of entry into DNA replication phase (S phase) via interaction with a replication initiation factor Mcm7 and the induction of centrosomal overduplication and chromosomal tetraploidization, which both could contribute to a breakdown of chromosomal homeostasis and to carcinogenesis. In this study we analyzed the molecular mechanisms and correlation of the aforementioned phenomena, and revealed that (1) CycA and Mcm7 simultaneously bind to chromosomal replication initiation regions in human cells, (2) aberrant activation of CycA-CDK induces the chromosome tetraploidization via an unknown pathway not involving Mps1, a regulatory factor for the centrosomal duplication and chromosomal segregation, and (3) the CycA function promoting S-phase entry is not involved in chromosomal tetraploidization.
|
Report
(4 results)
Research Products
(22 results)
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Enhancement of hyperthermia-induced tumor cell death by 5-aminolevulinic acid2012
Author(s)
Chibazakura, T., Toriyabe, Y., Takahashi, K., Kawakami, M., Ogura,S., Abe, F., Nakajima, M., Tanaka, T.
Organizer
The 11th international Congress of Hyperthermic Oncology & The 29th Japanese Congress of Thermal Medicine
Place of Presentation
ハイアットリージェンシー京都(京都府)
Year and Date
2012-08-31
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
